口腔医学研究

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (3): 270-273.DOI: 10.13701/j.cnki.kqyxyj.2018.03.014

• 颞下颌关节研究 • 上一篇    下一篇

人颞下颌关节骨关节炎滑膜成纤维细胞中血管生成及润滑相关因子的表达

郭慧琳1,2, 房维1,2, 邓末宏1, 龙星1,2*   

  1. 1. 武汉大学口腔医院第二颌面外科 湖北 武汉 430079;
    2. 武汉大学口腔医学院 口腔基础医学省部共建国家重点实验室培训基地和口腔生物医学教育部重点实验室 湖北 武汉 430079
  • 收稿日期:2018-01-22 出版日期:2018-03-30 发布日期:2018-03-27
  • 通讯作者: *龙星,E-mail: longxing@whu.edu.cn
  • 作者简介:郭慧琳(1988~ ),女,湖北武汉人,博士,主治医师,主要从事颞下颌关节疾病相关研究。
  • 基金资助:
    国家自然科学基金(编号:81470761;81771100);武汉大学青年教师(编号:2042017kf0140)

Expression of Angiogenic-associated and Lubricating-associated Factors in Synovial Fibroblasts of Temporomandibular Joint Osteoarthritis

GUO Hui-lin1,2, FANG Wei1,2, DENG Mo-hong1, LONG Xing<sup>1,2*   

  1. 1. Department of 2nd Oral and Maxillofacial Surgery, School of Stomatology, Wuhan University, Wuhan 430079, China;
    2. The State Key Laboratory Breeding Base of Basic Science of Stomatology and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, China
  • Received:2018-01-22 Online:2018-03-30 Published:2018-03-27

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摘要： 目的: 探究血管生成相关因子及润滑相关因子在颞下颌关节骨关节炎滑膜中的表达。方法: 分离人颞下颌关节滑膜组织,组织块法进行滑膜成纤维细胞的原代及传代培养。流式细胞仪检测细胞中CD34及CD44的表达,Western blotting 和荧光定量 PCR分别用于检测VEGF 和 TSP1 蛋白, PRG4, HAS2和HAS3基因的表达。 结果: 骨关节炎滑膜细胞中VEGF、CD34和HAS3的表达显著高于对照组,但TSP1蛋白、PRG4和HAS2 基因的表达低于对照组。结论: 在颞下颌关节骨关节炎滑膜成纤维细胞中,血管生成相关因子高表达,而提高润滑作用的基因表达降低,该现象可能与该病的疾病发展有关。

关键词: 润滑素, 透明质酸合成酶, 骨关节炎, 血管生成

Abstract: Objective: To investigate the expression of angiogenic-associated factors and lubricating-associated genes in the synovial fibroblasts of osteoarthritis (OA). Methods: Synovial tissues were collected from the temporomandibular joint of OA patients, and synovial fibroblasts were separated and cultured by tissue culture method. Flow cytometric analysis was used to examine the expression of CD34 and CD44. The protein expressions of VEGF and TSP1 were detected by western blotting. Real-time PCR was used to examined the gene expression of PRG4,HAS2 and HAS3. Results: The expressions of VEGF,CD34 and HAS3 were significantly increased in the synovial cells of OA, but TSP1, PRG4 and HAS2 expression were decreased. Conclusion: Proangiogenic factors were increased, and the lubricating-associated factors were decreased in the synovial cells of temporomandibular joint OA, suggesting that the synovium might play a beneficial role in the pathogenesis of OA by regulating the expression of these factors.

Key words: Lubricin, Hyaluronan synthase, Osteoarthritis, Angiogenesis