重复咬合干扰对大鼠咬肌痛觉敏感的易化作用

doi:10.13701/j.cnki.kqyxyj.2018.03.013

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (3): 266-269.DOI: 10.13701/j.cnki.kqyxyj.2018.03.013

重复咬合干扰对大鼠咬肌痛觉敏感的易化作用

莫思怡, 徐啸翔, 曹烨, 颉慧菲, 谢秋菲^*

北京大学口腔医学院·口腔医院,口腔修复科,口颌功能诊疗研究中心,口腔疾病国家临床研究中心,口腔数字化医疗技术和材料国家工程实验室,口腔数字医学北京市重点实验室北京 100081

收稿日期:2018-01-24 出版日期:2018-03-30 发布日期:2018-03-27
通讯作者: *谢秋菲,E-mail: xieqiuf@163.com
作者简介:莫思怡(1992~ ),女,湖南湘潭人,硕士在读 ,主要从事颞下颌关节疾病方面的研究。
基金资助:
国家自然科学基金(编号:81271174)

Repetitive Occlusal Interference Facilitates Masseter Hyperalgesia in Rats

MO Si-yi, XU Xiao-xiang, CAO Ye, JIE Hui-fei, XIE Qiu-fei^*

Department of Prosthodontics; Center for Oral Functional Diagnosis, Treatment, and Research; Peking University School and Hospital of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, Beijing 100081, China

Received:2018-01-24 Online:2018-03-30 Published:2018-03-27

摘要/Abstract

摘要： 目的: 分析初次咬合干扰对再次咬合干扰致大鼠咀嚼肌痛觉敏感的影响。方法: 于大鼠右上第一磨牙粘固0.4 mm厚金属冠,建立咬合干扰;初次干扰2 d后去除,再次干扰2 d或4 d后去除,测试2次干扰前后多个时间点双侧咬肌机械摆头阈值。结果: 初次干扰2 d去除后阈值需8 d恢复至基线;初次干扰2 d 后再次干扰2 d去除,阈值需10 d恢复至基线;初次干扰2 d后再次干扰4 d去除,阈值需19 d恢复至基线;初次假手术后再次干扰4 d去除,阈值仅需12 d恢复至基线。结论: 初次咬合干扰2 d对再次咬合干扰2 d 、4 d致咬肌痛敏有易化作用,表现在延长了再次咬合干扰致咬肌痛敏的恢复时间。本研究成功建立了重复咬合干扰易化咀嚼肌痛敏的动物模型。

关键词: 咀嚼肌, 痛觉敏感, 创伤性, 大鼠

Abstract: Objective: To investigate the influence of previous occlusal interference on masticatory muscle hyperalgesia induced by secondary occlusal interference in rats. Methods: Repetitive occlusal interferences were obtained by bonding a 0.4 mm thick metal crown on the upper first molar in rat and removing it after two or four days. The bilateral head withdrawal thresholds were measured at multiple time points. Results: Eight days were needed for the thresholds to recover to baseline after the removal of the first occlusal interference which lasted for two days, and 10 days were needed after two occlusal interferences which both lasted for two days. And it took respectively 19 days and 12 days for the thresholds to recover to baseline after the removal of the second occlusal interference which lasted for 4 days following the first occlusal interference which lasted for 2 days or following sham operation. Conclusion: The first occlusal interference which lasted for 2 days facilitated masseter hyperalgesia induced by the second occlusal interference which lasted for 2 or 4 days in postponing the recovery time without increasing the severity of masseter hyperalgesia. This study successfully established an animal model of repetitive occlusal interference-facilitated masticatory muscle pain.

Key words: Masticatory muscle, Hyperalgesia, Traumatic, Rats

莫思怡, 徐啸翔, 曹烨, 颉慧菲, 谢秋菲. 重复咬合干扰对大鼠咬肌痛觉敏感的易化作用[J]. 口腔医学研究, 2018, 34(3): 266-269.

MO Si-yi, XU Xiao-xiang, CAO Ye, JIE Hui-fei, XIE Qiu-fei. Repetitive Occlusal Interference Facilitates Masseter Hyperalgesia in Rats[J]. Journal of Oral Science Research, 2018, 34(3): 266-269.

参考文献

[1] 傅开元.2014年新版国际颞下颌关节紊乱病分类及诊断标准解读[J].中华口腔医学杂志,2017,52(6)∶374-376
[2] Chen W, Yin XM. Occlusal risk factors associated with temporomandibular disorders in young adults with normal occlusions [J]. Oral and Maxillofacial surgery, 2012, 114(4)∶419-423
[3] 张玲阁,龙星,蔡恒星.2种不同牙合垫治疗颞下颌关节紊乱病的临床疗效分析[J].口腔医学研究,2017,33(6)∶614-617
[4] Bell YL, Niemi PM, Jämsä T, et al. Subjective reactions to intervention with artificial interferences in subjects with and without a history of temporomandibular disorders [J]. Acta Odontologica Scandinavica, 2009, 64(1)∶59-63
[5] Li SG, Wang JY, Fei L. Adult-Age Inflammatory Pain Experience Enhances Long-Term Pain Vigilance in Rats [J]. PLos One, 2012, 7(5)∶e36767
[6] Ding TT, Xu XX, Cao Y, et al. Inflammatory pain memory facilitates occlusal interference-induced masticatory muscle hyperalgesia in rats [J]. Eur J Pain, 2016, 20(3)∶353-364
[7] Cao Y, Xie QF, Li K, et al. Experimental occlusal interference induces long-term masticatory muscle hyperalgesia in rats [J]. Pain, 2009, 144 (3)∶287-293
[8] 徐啸翔,曹烨,傅开元,等.咬合干扰致大鼠咬肌能量代谢产物含量变化[J].北京大学学报(医学版)医学版,2017,49(1)∶25-30
[9] Palla S, Farella M. Masticatory Muscle Pain [M]. Muscle Pain Diagnosis & Treatment, 2010∶193-227
[10] 李雪姣,曹烨,谢秋菲.牙合干扰致大鼠咀嚼肌机械痛觉过敏的特点研究[J].中华口腔医学杂志,2014,49(10)∶596-599
[11] Kuffler DP. Coping with Phantom Limb Pain [J]. Molecular Neurobiology, 2017(Pt A)∶1-15
[12] Noel M, Chambers CT, Mcgrath P J, et al. The influence of children’s pain memories on subsequent pain experience [J]. Pain, 2012, 153(8)∶1563-1572
[13] Noel M, Chambers CT, Petter M, et al. Pain is not over when the needle ends: a review and preliminary model of acute pain memory development in childhood [J]. Pain Management, 2012, 2(5)∶487-497
[14] Althaus A, Hinrichs-Rocker A, Chapman R, et al. Development of a risk index for the prediction of chronic post-surgical pain [J]. European Journal of Pain, 2012, 16(6)∶901-910
[15] Reyes-Castro LA, Padilla-Gómez E, Parga-Martinez NJ, et al. Hippocampal mechanisms in impaired spatial learning and memory in male offspring of rats fed a low-protein isocaloric diet in pregnancy and/or lactation [J]. Hippocampus, 2017, 28(1)∶18-30
[16] Bliss TV, Collingridge GL. A synaptic model of memory: long-term potentiation in the hippocampus [J]. Nature, 1993, 361(6407)∶31-39

重复咬合干扰对大鼠咬肌痛觉敏感的易化作用

Repetitive Occlusal Interference Facilitates Masseter Hyperalgesia in Rats

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