口腔医学研究

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (7): 726-729.DOI: 10.13701/j.cnki.kqyxyj.2018.07.010

• 口腔种植学研究 • 上一篇    下一篇

大鼠微螺钉种植体周围炎外周血中Foxp3+调节性T细胞的水平变化

李娜1, 林崇翔2, 丁熙2, 邵侠2, 赵瑜1, 屠呈威2*   

  1. 1. 温州医科大学附属口腔医院牙体牙髓科 浙江 温州 325000;
    2. 温州医科大学附属第一医院口腔颌面外科 浙江 温州 325000
  • 收稿日期:2018-01-22 出版日期:2018-07-28 发布日期:2018-07-20
  • 通讯作者: 屠呈威,E-mail:175193674@qq.com
  • 作者简介:李娜(1986~ ),女,浙江宁波人,硕士,主治医师, 研究方向为口腔种植与修复。
  • 基金资助:
    温州市科技计划项目(编号:Y20140325) 温州市科技计划项目(编号:Y20170762)

Change of Foxp3+ Regulatory T Cells Level in Peripheral Blood of Experimental Peri-implantitis Model of Rats with Microscrew Implants

LI Na1, LIN Chong-xiang2, DING Xi2, SHAO Xia2, ZHAO Yu1, TU Cheng-wei2*   

  1. 1. Department of Endodontics, the Affiliated Stomatological Hospital, Wenzhou Medical University, Wenzhou 325000, China;
    2. Department of Oral-maxillofacial Surgery, the First Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, China.
  • Received:2018-01-22 Online:2018-07-28 Published:2018-07-20

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摘要： 目的:建立大鼠股骨微螺钉种植体周围炎模型,检测其外周血中Foxp3+调节性T细胞水平的变化。方法:12只雄性SD大鼠分为正常组、炎症组、空白组(n=4),正常组和炎症组单侧股骨远心端植入微螺钉种植体1枚,炎症组在植入种植体后用丝线环扎种植体颈部并使种植体上端暴露于体表外;正常组在植入种植体后严密缝合,植体不外露;空白组仅进行麻醉、切开及缝合等对照处理,未植入种植体。术后第4周取样、采血,拍摄X线片并采用流式细胞术检测外周血中Foxp3+调节性T细胞(CD4+CD25+Foxp3+)在T淋巴细胞(CD4+)中的比例。结果: 炎症组大鼠种植体周围出现炎性肉芽组织,X线片显示植体颈部周围皮质骨有角形吸收,其外周血中Foxp3+调节性T细胞占CD4+T淋巴细胞的比例为(0.66±0.32)%,显著低于正常组(2.69±1.19)%和空白组(4.06±1.34)%,差异有统计学意义(P<0.05);正常组和对照组之间差异无统计学意义。结论: 大鼠股骨远心端微螺钉种植体周围炎模型造模成功;该动物模型外周血中Foxp3+调节性T细胞在数量上存在异常,可能与种植体周围炎的发生有关。

关键词: 微螺钉种植体, 种植体周围炎, 调节性T细胞, Foxp3, 大鼠

Abstract: Objective: To establish a peri-microscrew implantitis model in the femur of rats, and investigate the change of proportion of Foxp3+ regulatory T cells in peripheral blood. Methods: Twelve male SD rats were divided into normal group, peri-implantitis group, and blank group (n=4). The normal group and peri-implantitis group accepted an implantation of microscrew implant in the distal end of the femur, while the later one underwent cervical ligation immediately around the implants whose upper part was exposed in vitro. The normal group had no events after the implantation, and the blank group only accepted a “fake” operation without implantation. Four weeks after the operation, the abdominal blood was collected and the femur of each group was taken an X-ray examination and detected the the ratio of Foxp3+ regulatory T cells (CD4+CD25+Foxp3+) in T lymphocytes (CD4+) using flow cytometry. Results: The peri-implantitis group showed an inflammatory granulation tissue around the implants, whose X-ray film indicated an angular absorption of cortical bone and had a lower proportion (0.66±0.32)% of Foxp3+ regulatory T cells in the peripheral blood, significantly lower than normal group (2.69±1.19)% and blank group (4.06±1.34)% (P<0.05). There was no statistically significant difference between normal group and the control group. Conclusion: The model of peri-microscrew implantitis in rats’ distal end of femur can be established. Rats with peri-microscrew implantitis have a decrease in the number of Foxp3+ regul-atory T cells in peripheral blood, which may be associated with the pathogenisis of peri-microscrew implantitis.

Key words: Microscrew implant, Peri-implantitis, Regulatory T-lymphocytes, Foxp3, Rat