口腔医学研究

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (7): 721-725.DOI: 10.13701/j.cnki.kqyxyj.2018.07.009

• 口腔种植学研究 • 上一篇    下一篇

浓缩生长因子促进大鼠种植体骨结合的实验研究

葛林虎, 方颖, 查骏, 李军, 万绵佳, 王丽萍*   

  1. 广州医科大学附属口腔医院 广州口腔疾病研究所 口腔医学重点实验室 广东 广州 510140
  • 收稿日期:2018-02-25 出版日期:2018-07-28 发布日期:2018-07-20
  • 通讯作者: 王丽萍,E-mail: wangliplj@126.com
  • 作者简介:葛林虎(1963~ ),男,湖北人,博士,主任医师,主要从事组织工程及肿瘤靶向治疗的研究。
  • 基金资助:
    广东省科学技术厅项目(编号:2013B021800186)广州市卫生局项目(编号:20142A011025 )

Effects of Concentrated Growth Factors on Osseointegration of Titanium Implants in Rats

GE Lin-hu, FANG Yin, ZHA Jun, LI Jun, WAN Mian-jia, WANG Li-ping*   

  1. Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medical University, Guangzhou 510140, China.
  • Received:2018-02-25 Online:2018-07-28 Published:2018-07-20

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摘要： 目的:研究浓缩生长因子对成骨细胞骨唾液酸蛋白(bone sialoprotein,BSP)、runt转录相关因子2(runt related transcription factor 2,Runx2)、碱性磷酸酶(alkaline phosphatase,ALP)基因表达的影响,探讨浓缩生长因子促进大鼠种植体骨结合的作用机制。方法:采用噻唑蓝比色法(methylthiazolyldiphenyl-tetrazolium bromide,MTT)和实时定量反转录聚合酶链反应(real-time reverse transcriptase-polymerase chain reaction,RT-qPCR)法检测浓缩生长因子对成骨细胞增殖和BSP、Runx2、ALP基因表达的影响;建立大鼠股骨种植体骨结合模型,应用Micro-CT扫描测定标本骨矿密度、骨体积百分比等数据。结果: 浓缩生长因子可以促进成骨细胞的增殖和成骨分化相关基因BSP、Runx2、ALP的表达。种植体植入8周后,与对照组比较,浓缩生长因子组骨矿密度、骨体积百分比、骨小梁数量和成骨界面面积均显著升高。结论: 浓缩生长因子可能通过增加成骨相关基因表达而促进大鼠种植体骨结合,浓缩生长因子是一种安全、无排斥反应的理想生物膜材料。

关键词: 浓缩生长因子, 成骨细胞, 牙种植

Abstract: Objective: To study the effects of concentrated growth factors on the expression of BSP, Runx2, and ALP, and to seek the mechanism of concentrated growth factors stimulating the osseointegration of titanium implants in rats. Methods: In vitro experiment, MTT and RT-qPCR were used to detect the proliferation and the expression of BSP, Runx2, and ALP in osteoblasts. In vivo experiment, the model of the osseointegration of titanium implants in the femora of rats was established. Micro-CT was used to analyze the bone mineral density, percent bone volume, and intersection surface. Results: Concentrated growth factors significantly increased the proliferation and the expression of BSP, Runx2, and ALP in osteoblasts. In vivo experiment, 8 weeks after implantation, concentrated growth factors significantly increased the bone mineral density, percent bone volume, and intersection surface. Conclusion: Concentrated growth factor may promote the osseointegration of titanium implants in rats by increasing the expression of osteoblast differentiation related genes, such as BSP, Runx2, and ALP.

Key words: Concentration growth factor, Osteoblast Implant