口腔医学研究

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (9): 948-951.DOI: 10.13701/j.cnki.kqyxyj.2018.09.007

• 口腔肿瘤学研究 • 上一篇    下一篇

芦荟大黄素纳米脂质体介导的光动力疗法对人舌鳞状细胞癌细胞Tca-8113治疗效果的研究

邵明敬,王赫,董丽,张红超,刘旭,孟培松*   

  1. 哈尔滨医科大学附属第四医院口腔科 黑龙江 哈尔滨 150001
  • 收稿日期:2018-03-07 出版日期:2018-09-28 发布日期:2018-09-25
  • 通讯作者: 孟培松,E-mail:mengpeisong641205@163.com
  • 作者简介:邵明敬(1990~ ),男,山东聊城人,硕士在读,主要从事口腔颌面外科肿瘤方向。
  • 基金资助:
    哈尔滨市科学技术局项目(编号:2014RFXGJ095)

Therapeutic Effect of Aloe-emodin Nanometer Liposome-mediated Photodynamic Therapy on Human Tongue Squamous Cell Carcinoma Tca-8113 Cells.

SHAO Ming-jing , WANG He, DONG Li, ZHANG Hong-chao, LIU Xu, MENG Pei-song*.   

  1. Department of Stomatology, the Fourth Medical College of Harbin Medical University, Harbin 150001, China.
  • Received:2018-03-07 Online:2018-09-28 Published:2018-09-25

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摘要： 目的: 探究芦荟大黄素纳米脂质体(aloe emodin nano-liposome,AE-L)介导的光动力疗法(photodynamic therapy,PDT)对人舌鳞状细胞癌(简称鳞癌)细胞Tca-8113的治疗效果。方法: 逆相蒸发法制备AE-L;不同浓度AE-L(2.5、5.0、7.5、10.0、12.5 μg/mL)作用Tca-8113细胞4 h后,不同光照时间处理(0、10、20、40、80、160 s;激发波长为405 nm,功率密度为80 mW/cm2)Tca-8113,采用噻唑蓝(methyl thiazol tetrazolium,MTT)检测细胞存活率;AE-L(7.5 μg/mL)作用细胞4 h后,光照时间为80 s(激发波长为405 nm,功率密度为80 mW/cm2)处理细胞,荧光显微镜定性分析PDT后活性氧(reactive oxygen species,ROS)含量。结果: AE-L浓度≤7.5 μg/mL时细胞毒性较小,与对照组比较差异无统计学意义(P＞0.05)。PDT组随着光照时间延长细胞存活率逐渐降低,光照时间80 s时细胞存活率为76.6%,与对照组比较差异有统计学意义(P＜0.001)。荧光染色结果显示,PDT治疗后细胞内ROS含量显著增加。结论: 7.5 μg/mL AE-L介导的PDT治疗对人舌鳞癌Tca-8113细胞具有显著的杀伤效果。

关键词: 芦荟大黄素, 脂质体, 人舌鳞状细胞癌, 光动力疗法, 活性氧

Abstract: Objective: To study the therapeutic effect of aloe-emodin nano-liposome-mediated photodynamic therapy (PDT) on human tongue squamous cell carcinoma Tca-8113 cells. Methods: Aloe-emodin nanosized liposomes (AE-L) were prepared by reverse phase evaporation method. Aloe-emodin absorption spectra were measured by ultraviolet spectrophotometer. The effects of different concentrations of AE-L (2.5, 5.0, 7.5, 10.0, and 12.5 μg/mL) Tca-8113 cells were treated with different light time (0, 10, 20, 40, 80, and 160 s; excitation wavelength at 405 nm; power density at 80 mW/cm2). Cell viability was detected by MTT assay. After AE-L (7.5 μg/mL) treated for 4 h, the irradiation time was 80 s, the ROS content was analyzed by fluorescence microscopy. Results: When the concentration of AE-L was less than 7.5 μg/mL, there was not difference between PDT group and control group (P>0.05). With the increase of irradiation time, the cell survival rate decreased gradually.When the illumination time was 80 s, the cell survival rate was 76.6%, which is significant different from that of the control group (P<0.001). The results of fluorescent staining showed that the amount of ROS in the cells increased significantly after PDT treatment. Conclusion: The 7.5 μg/mL AE-L-mediated PDT has a significant killing effect on human tongue squamous cell carcinoma Tca-8113 cells.

Key words: Aloe emodin , Liposome , Human tongue squamous cell carcinoma , Photodynamic therapy, Reactive oxygen species