口腔医学研究

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (9): 943-947.DOI: 10.13701/j.cnki.kqyxyj.2018.09.006

• 口腔肿瘤学研究 • 上一篇    下一篇

口腔鳞状细胞癌外泌体mRNA表达图谱分析

胡慧珍1,王世琴1,孙贵军1,金佳佳1,李志勇2,何祥一1*   

  1. 1. 兰州大学口腔医学研究所 甘肃 兰州 730000;
    2. 中国农业科学院兰州兽医研究所 甘肃 兰州 730046
  • 收稿日期:2018-03-10 出版日期:2018-09-28 发布日期:2018-09-25
  • 通讯作者: 何祥一,E-mail addr.: hexy@lzu.edu.cn
  • 作者简介:胡慧珍(1990~ ),女,甘肃人,硕士,主要研究方向为口腔医学基础及临床医学研究。
  • 基金资助:
    国家自然科学基金(编号:31572522);甘肃省自然科学基金(编号:17JR5RA217)

Analysis of mRNA Expression of Exosomes Derived from Oral Squamous Cell Carcinoma.

HU Hui-zhen1, WANG Shi-qin1, SUN Gui-jun1, JIN Jia-jia1, LI Zhi-yong2, HE Xiang-yi1*   

  1. 1.School of Stomatology, Lanzhou University, Lanzhou 730000, China;
    2. Lanzhou Veterinary Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
  • Received:2018-03-10 Online:2018-09-28 Published:2018-09-25

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摘要： 目的: 研究口腔鳞状细胞癌细胞(oral squamous cell cancer cells, OSCC))外泌体与正常口腔上皮细胞(Human immortalized oral epithelial cell, HOEC)外泌体mRNA之间的表达差异,通过特异性外泌体mRNA表达图谱分析,为探究口腔鳞状细胞癌的发生发展机制打下一定的研究基础。方法: 利用高通量测序技术检测CAL-27和HOEC外泌体mRNA表达谱,筛选出差异mRNA,对差异mRNA进行GO(Gene Ontoloty)分析和KEGG通路分析。结果: 口腔鳞状细胞癌细胞外泌体中检测到mRNA 44699 条,口腔上皮细胞外泌体中mRNA数量为44761条,其中,差异表达的mRNA共471条(234个上调,237个下调)。生物信息学分析表明上述差异表达的mRNA参与了口腔癌的生物学调节功能和通路。结论: 口腔鳞癌细胞及口腔上皮细胞均能产生丰富的外泌体;口腔鳞状细胞癌细胞外泌体与正常口腔上皮细胞外泌体间mRNA表达谱存在较大差异,差异表达的mRNA在肿瘤的发生、发展及转移过程中可能有重要作用。

关键词: 口腔癌, 外泌体, mRNA, 高通量分析

Abstract: Objective: To compare the mRNA expression between the exosomes derived from oral squamous cell cancer cells (OSCC) and human immortalized oral epithelial cell (HOEC), and to explore the evolutionary mechanism of OSCC by the approach of bioinformatic analysis. Methods: High throughput sequencing technology was used to explore mRNA expression profile in the exosomes of OSCC and HOEC. The differently expressed genes were selected and analyzed by Gene Ontoloty (GO) analysis and KEGG pathway analysis. Results: Among 44699 genes detected in the exosomes of OSCC and HOEC,471 differently expressed mRNAs were found (234 mRNAs were up-regulated and 237 mRNAs were down-regulated). Bioinformatics analysis showed that differently expressed mRNAs played an important role in the biological regulation and pathway of OSCC. Conclusion: Abundant exosomes can be secreted by OSCC and HOEC. The mRNA expression profiles of exosome from OSCC are quite different from those of HOEC, which play an important role in the occurrence, development, and metastasis of tumor. Exploring mRNA expression profiles of the exosomes derived from two different cells can provide new ideas and methods for understanding the evolutional mechanism of oral squamous cell cancer.

Key words: Oral cancer , Exosome , mRNA , High throughput sequencing technology