长链非编码RNA FAL1在口腔鳞状细胞癌中的表达及其与预后的关系

doi:10.13701/j.cnki.kqyxyj.2019.04.013

口腔医学研究 ›› 2019, Vol. 35 ›› Issue (4): 360-363.DOI: 10.13701/j.cnki.kqyxyj.2019.04.013

长链非编码RNA FAL1在口腔鳞状细胞癌中的表达及其与预后的关系

白真玉¹, 王铠², 刘德裕¹, 黄谢山^1*

1. 海口市人民医院,中南大学湘雅医学院附属海口医院口腔外科海南海口 570208;
2. 中南大学湘雅二医院口腔科湖南长沙 410011

收稿日期:2018-08-29 出版日期:2019-04-28 发布日期:2019-04-23
通讯作者: 黄谢山,E-mail:hnsun3@126.com
作者简介:白真玉(1983~ ),女,硕士,主治医师,主要从事口腔颌面外科、口腔肿瘤研究工作。
基金资助:
海南省医药卫生科研项目(编号:1601320443A2001);

Expression of LncRNA FAL1 in Oral Squamous Cell Carcinoma and Its Relationship with Prognosis

BAI Zhen-yu¹, WANG Kai², LIU De-yu¹, HUANG Xie-shan^1*

1.Department of Oral Surgery, Haikou People's Hospital (Haikou Hospital Affiliated to Xiangya Medical College of Central South University), Haikou 570208, China;
2.Department of Stomatology, Xiangya No.2 Hospital of Central South University, Changsha 410011, China

Received:2018-08-29 Online:2019-04-28 Published:2019-04-23

摘要/Abstract

摘要： 目的: 探讨长链非编码RNA(long noncoding,lncRNA)FAL1在口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)中的表达及其与预后的关系。方法: 纳入2013年1月~2015年5月海口市人民医院和中南大学湘雅二医院收治的95例口腔鳞状细胞癌患者作为研究对象,以手术切除的口腔鳞状细胞癌组织为口腔鳞状细胞癌组,以50例健康人口腔黏膜组织作为对照组。采用逆转录-聚合酶链反应(reverse transcription-polymerase chain reaction,RT-PCR)检测lncRNA FAL1在两组的表达水平;收集患者的临床病理资料,按照lncRNA FAL1表达结果的中位值将OSCC组分为低表达组和高表达组,分析lncRNA FAL1的表达与临床病理特征的关系;采用Kaplan-Meier法进行生存分析,Log-rank法检验lncRNA FAL1的表达水平对患者预后的影响;对影响OSCC发生的相关因素进行Cox分析。结果: OSCC组lncRNA FAL1表达水平显著高于对照组(P＜0.05),lncRNA FAL1与临床分期、T分期、分化程度、淋巴转移显著相关(P＜0.05);Kaplan-Meier生存分析显示lncRNA FAL1低表达组无进展生存期(progression free survival,PFS)、总体生存时间(overall survival,OS)显著高于高表达组(P＜0.05);Cox分析结果显示临床分期、T分期、分化程度、淋巴转移均是影响OSCC发生的危险因素。结论: OSCC患者组织中lncRNA FAL1高表达,可能与OSCC的发生、发展及预后密切相关。

关键词: 口腔鳞状细胞癌, 长链非编码RNA, FAL1, 临床意义, 预后

Abstract: Objective: To investigate the expression of long-chain non-coding RNA (LncRNA) FAL1 in oral squamous cell carcinoma (OSCC) and its relationship with prognosis. Methods: Ninty-five patients with OSCC admitted to Haikou People's Hospital and Xiangya No.2 Hospital of Central South University from January 2013 to May 2015 were enrolled in this study. The OSCC tissues surgically resected were set as OSCC group, and oral mucosal tissues of 50 healthy people were set as control group. Real time quantitative PCR was used to detect the expression level of LncRNA FAL1 in the two groups. The clinicopathological data of patients were collected. According to the median value of LncRNA FAL1 expression, OSCC group was divided into low expression group and high expression group. The relationship between the expression of LncRNA FAL1 and clinicopathological characteristics was analyzed. Kaplan-Meier method was used for survival analysis. Log-Rank method was used to examine the effect of LncRNA FAL1 expression on prognosis. Cox was used to analyze the related factors affecting OSCC. Results: The expression of LncRNA FAL1 in OSCC group was significantly higher than that in control group (P<0.05). LncRNA FAL1 was significantly correlated with clinical stage, T stage, differentiation degree, and lymphatic metastasis (P<0.05). Kaplan-Meier survival analysis showed that PFS and OS in LncRNA FAL1 low expression group were significantly higher than those in LncRNA FAL1 high expression group (P<0.05). Cox analysis showed that clinical stage, T stage, degree of differentiation, and lymphatic metastasis were all risk factors for OSCC. Conclusion: LncRNA FAL1 is highly expressed in tissues of patients with OSCC, and it may be closely related to the occurrence, development, and prognosis of OSCC.

Key words: Oral squamous cell carcinoma, Long-chain non-coding RNA FAL1, Clinical significance, Prognosi

白真玉, 王铠, 刘德裕, 黄谢山. 长链非编码RNA FAL1在口腔鳞状细胞癌中的表达及其与预后的关系[J]. 口腔医学研究, 2019, 35(4): 360-363.

BAI Zhen-yu, WANG Kai, LIU De-yu, HUANG Xie-shan. Expression of LncRNA FAL1 in Oral Squamous Cell Carcinoma and Its Relationship with Prognosis[J]. Journal of Oral Science Research, 2019, 35(4): 360-363.

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长链非编码RNA FAL1在口腔鳞状细胞癌中的表达及其与预后的关系

Expression of LncRNA FAL1 in Oral Squamous Cell Carcinoma and Its Relationship with Prognosis

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