甲基硒酸对口腔癌细胞增殖、迁移、凋亡和周期的作用研究

doi:10.13701/j.cnki.kqyxyj.2019.06.014

口腔医学研究 ›› 2019, Vol. 35 ›› Issue (6): 568-572.DOI: 10.13701/j.cnki.kqyxyj.2019.06.014

甲基硒酸对口腔癌细胞增殖、迁移、凋亡和周期的作用研究

周童¹,张桐菲²,张泽兵¹,全海英^3*

1. 吉林大学口腔医院病理科,吉林省牙发育及颌骨重塑与再生重点实验室吉林长春 130021;
2. 吉林大学口腔医院医务科吉林长春 130021;
3. 吉林医药学院附属医院口腔科吉林吉林 132013

收稿日期:2018-10-09 出版日期:2019-06-28 发布日期:2019-06-27
通讯作者: 全海英,E-mail:hanbingsui111@163.com
作者简介:周童(1993~ ),女,山东济宁人,硕士在读,主要从事口腔颌面部肿瘤发病机制及其治疗方面的研究。
基金资助:
吉林省教育厅“十三五”科学技术项目(编号:JJKH20180834KJ)
吉林省科技厅国际科技合作项目(编号:20190701071GH)

Influence of MSA on Proliferation, Migration, Apoptosis, and Cell Cycle of Oral Squamous Cell Carcinoma Tca8113 and KB Cells

ZHOU Tong¹, ZHANG Tong-fei², ZHANG Ze-bing¹, QUAN Hai-ying^3*

1. Department of Oral Pathology, School and Hospital of Stomatology, Jilin University; Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Changchun 130021, China;
2. Medical Administration, Department School and Hospital of Stomatology, Jilin University, Changchun 130021, China;
3. Department of Stomatology, Affiliated Hospital of Jilin Medical University, Jilin 132013, China.

Received:2018-10-09 Online:2019-06-28 Published:2019-06-27

摘要/Abstract

摘要： 目的:利用甲基硒酸(methylseleninic acid,MSA)作用于口腔鳞状细胞癌Tca8113和KB细胞,探讨MSA对口腔癌细胞的增殖、迁移、凋亡和周期的影响。方法:体外培养口腔癌细胞,加入不同浓度的MSA培养不同时间,MTS 法及细胞克隆法测定细胞增殖情况;流式细胞仪检测细胞凋亡率和细胞周期分布;划痕实验测定细胞的迁移情况。结果:MSA可显著抑制口腔癌细胞的增殖和迁移,作用效果随药物浓度增加而增强;MSA可诱导口腔癌细胞体外凋亡,随着药物浓度的增加, 口腔癌细胞凋亡率逐渐增加。MSA可改变口腔癌细胞的细胞周期, 将口腔癌细胞周期阻断在G₂期,并且减少G₁期和S期细胞的比例。结论:MSA可显著抑制口腔癌细胞的体外增殖、迁移,并促进其凋亡,改变口腔癌细胞周期。MSA有望成为治疗口腔癌的一种新型药物。

关键词: 甲基硒酸, 口腔鳞状细胞癌, 增殖, 凋亡, 迁移, 细胞周期

Abstract: Objective: To explore the influence of MSA on the proliferation, migration, apoptosis, and cell cycle of oral squamous cell carcinoma Tca8113 and KB cells. Methods: MTS assay was used to detect cell proliferation activity. Wound healing assay was used to detect the capability of cell migration. The cell cycle distribution and apoptotic rate were measured using flow cytometry assay. Results: MSA inhibited the proliferation of Tca8113 and KB cells and reduced the cell migration. MSA suppressed the apoptosis of Tca8113 and KB cells markedly in a dose dependent manner. Cell cycle was blocked at G2 stage and the number of cell at G1 and S stage was reduced when stimulated with MSA. Conclusions: MSA can inhibit the proliferation and migration, induce the apoptosis, and change cell cycle of oral squamous cell carcinoma Tca8113 and KB cells in vitro. MSA is expected to be a new method to prevent and treat the oral cancer.

Key words: MSA, Oral squamous cell carcinoma, Proliferation, Apoptosis, Migration, Cell cycle

周童,张桐菲,张泽兵,全海英. 甲基硒酸对口腔癌细胞增殖、迁移、凋亡和周期的作用研究[J]. 口腔医学研究, 2019, 35(6): 568-572.

ZHOU Tong, ZHANG Tong-fei, ZHANG Ze-bing, QUAN Hai-ying. Influence of MSA on Proliferation, Migration, Apoptosis, and Cell Cycle of Oral Squamous Cell Carcinoma Tca8113 and KB Cells[J]. Journal of Oral Science Research, 2019, 35(6): 568-572.

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甲基硒酸对口腔癌细胞增殖、迁移、凋亡和周期的作用研究

Influence of MSA on Proliferation, Migration, Apoptosis, and Cell Cycle of Oral Squamous Cell Carcinoma Tca8113 and KB Cells

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