口腔医学研究

口腔医学研究 ›› 2020, Vol. 36 ›› Issue (3): 235-238.DOI: 10.13701/j.cnki.kqyxyj.2020.03.011

• 修复材料抗菌性研究 • 上一篇    下一篇

不同种植体表面改性对细菌生物膜的影响

汪汉池, 孙晓琳, 方蛟, 杜留熠, 彭培轩, 周延民*   

  1. 吉林大学口腔医学院种植中心 吉林 长春 130021
  • 收稿日期:2019-10-11 出版日期:2020-05-15 发布日期:2020-05-15
  • 通讯作者: 周延民,E-mail:zhouyanmin62@126.com
  • 作者简介:汪汉池(1994~),女,沈阳人,硕士,主要从事骨增量技术方面的研究。
  • 基金资助:
    国家自然科学基金(编号:3A415CY93431)

Effect of Surface Modification of Different Implants on Bacterial Biofilm

WANG Hanchi, SUN Xiaolin, FANG Jiao, DU Liuyi, PENG Peixuan, ZHOU Yanmin*   

  1. Department of Oral Implantology, School and Hospital of Stomatology, Jilin University, Changchun 130021, China
  • Received:2019-10-11 Online:2020-05-15 Published:2020-05-15

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摘要： 目的：研究临床常用的3种钛种植体不同表面处理对口腔内常见菌群牙龈卟啉单胞菌(Porphyromanusgingivalis,P. gingivalis)、具核梭杆菌(Fusobacterium nucleatum,F.nucleatum)及血链球菌(Streptococcus sanguis,S.sanguis)生物膜黏附的影响。方法：将钛片分成3组进行表面改性处理,分别为:电解沉积羟基磷灰石(HA)组、微弧氧化(MAO)组和大颗粒喷砂酸蚀(SLA)组,进行表面性能检测;在3组钛片上分别培养Pg、Fn及Ss 4 d,通过菌落形成单位(CFU)法、噻唑蓝(MTT)法和扫描电子显微镜(SEM)探究细菌的黏附情况。结果：(1)SEM观察表面形貌可见HA组表面大量不规则的棒状结构;MAO表面“火山口”样结构,孔径在0.1~1 μm之间;SLA表面为不规则凹陷,大小不一且边缘锋利。3组表面粗糙度Ra值:SLA组<HA组<MAO组;MAO组与另外2组有统计学差异(P<0.05)(2)Fn生物膜在SLA组数量最低,与另外2组有统计学差异(P<0.05);Ss生物膜在3组钛片上活性为HA组最高SLA组最低,但3组间无统计学差异(P>0.05);Pg生物膜在MAO组钛片上活性最高,与另外2组有统计学差异(P<0.01)。(3)利用SEM观察显示,细菌活性与种植体表面形态结构有关。结论：细菌在种植体表面黏附受到表面形貌影响,与粗糙度及物理构型有关。对于Pg、Ss和Fn 3种细菌,SLA在3种表面处理中细菌粘附量最少。

关键词: 钛种植体, 表面改性, 细菌生物膜

Abstract: Objective: To study the effects of surface treatments commonly used in clinic on the adhesion of Porphyroman gingivalis (Pg), Fusobacterium nucleatum (Fn), and Streptococcus sanguis (Ss). Methods: Titanium discs were divided into three groups for surface modification: electrodeposited hydroxyapatite (HA) group, micro-arc oxidation (MAO) group, and sandblasted with large-grit and acid-etched (SLA) group. The surface properties were tested. Fn, Ss, and Pg were cultured on three groups of titanium discs for 4 days, respectively. The colony forming unit (CFU) method, thiazole blue (MTT) method, and scanning electron microscopy (SEM) were used to observe bacterial adhesion. Results: (1) SEM observation showed that there were a large number of irregular rod-like structures on the surface of HA group, crater-like structures on the surface of MAO with aperture ranging from 0.1 to 1 micron, and irregular depressions on the surface of SLA with different sizes and sharp edges. Ra values of surface roughness in three groups was: SLA group < HA group < MAO group; (2) Fn biofilm was the lowest in SLA group, with statistical difference from the other two groups (P<0.05); The activity of Ss biofilm on three groups of titanium discs was the highest in HA group and the lowest in SLA group, but there was no significant difference among three groups (P>0.05). Pg biofilm had the highest activity on titanium plate in MAO group, with statistical difference from the other two groups (P<0.01). (3) SEM observation showed that the bacterial activity was related to the morphological structure of implant surface. Conclusion: The adhesion of bacteria to implant surface is affected by surface morphology, which is related to roughness and physical configuration. For three kinds of bacteria, SLA had the least bacterial adhesion.

Key words: titanium implant, surface modification, bacterial biofilm