人牙周膜细胞和牙周膜组织的初级纤毛观察

doi:10.13701/j.cnki.kqyxyj.2016.02.006

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (2): 131-133.DOI: 10.13701/j.cnki.kqyxyj.2016.02.006

人牙周膜细胞和牙周膜组织的初级纤毛观察

尹凤英¹ 孙慧芳¹ 王敏¹ 张璠² 程祥荣¹ 夏海斌^1*

1. 武汉大学口腔医学院种植科湖北武汉 430079;
2. 中国科学院水生生物研究所湖北武汉 430070

收稿日期:2015-07-29 出版日期:2016-02-28 发布日期:2016-03-10
通讯作者: 夏海斌,E-Mail:xhaibin@whu.edu.cn
作者简介:尹凤英(1988~),女,山东人,硕士在读,主要从事口腔临床及基础的研究工作。
基金资助:
国家自然科学基金资助项目(批号:81271179)

Observation on Primary Cilia of Human Periodontal Ligament Cells and Periodontal Tissue.

YIN Feng-ying¹, SUN Hui-fang¹, WANG min¹, ZHANG Fan², CHENG Xiang-rong¹, XIA Hai-bin¹

1. School of Stomatology, Wuhan University, Wuhan 430079, China;
2. Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430079, China

Received:2015-07-29 Online:2016-02-28 Published:2016-03-10

摘要/Abstract

摘要： 目的:观察牙周膜组织和体外培养的牙周膜细胞的初级纤毛情况。方法:切取人牙根中1/3的牙周膜组织的冰冻切片和切取小鼠切牙牙周膜组织石蜡切片,原代培养人牙周膜细胞至P3,采用acet-α-tubulin利用间接免疫荧光的方法观察初级纤毛结构。结果:人及小鼠牙周膜组织以及人牙周膜细胞均可见绿色短棒状荧光信号,位于细胞核附近,长度约2~3 μm。结论:牙周膜组织及体外培养的人牙周膜细胞均含有初级纤毛结构

关键词: 牙周膜, 人牙周膜细胞, 初级纤毛

Abstract: Objective: To observe primary cilia in periodontal tissue and human periodontal ligament cells (hPDLCs) cultured in vitro. Methods: The ligaments of human and mouse were cut from the middle of roots and made into frozen and paraffin section respectively. hPDLCs were cultured in vitro. Then acet-α-tubulin antibody was used to detect the primary cilia of periodontal tissue and cells. Results: The signal of primary cilia was short rod near to the nuclei in periodontal ligament and primary hPDLCs. The length of most primary cilia was about 2-3 μm. Conclusion: Both periodontal ligament and primary hPDLCs have the primary cilia.

中图分类号:

R781.4

尹凤英,孙慧芳,王敏,张璠,程祥荣,夏海斌. 人牙周膜细胞和牙周膜组织的初级纤毛观察[J]. 口腔医学研究, 2016, 32(2): 131-133.

YIN Feng-ying, SUN Hui-fang, WANG min, ZHANG Fan, CHENG Xiang-rong, XIA Hai-bin. Observation on Primary Cilia of Human Periodontal Ligament Cells and Periodontal Tissue.[J]. Journal of Oral Science Research, 2016, 32(2): 131-133.

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人牙周膜细胞和牙周膜组织的初级纤毛观察

Observation on Primary Cilia of Human Periodontal Ligament Cells and Periodontal Tissue.

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