高糖对牙龈卟啉单胞菌LPS引发牙周膜细胞炎症反应的影响

doi:10.13701/j.cnki.kqyxyj.2020.12.010

口腔医学研究 ›› 2020, Vol. 36 ›› Issue (12): 1123-1127.DOI: 10.13701/j.cnki.kqyxyj.2020.12.010

高糖对牙龈卟啉单胞菌LPS引发牙周膜细胞炎症反应的影响

孙天祥^1,2, 苗棣^1,2*, 王宝彦², 陈悦²

1.西安交通大学口腔医院陕西省颅颌面精准医学研究重点实验室陕西西安 710004;
2.西安交通大学口腔医院牙周黏膜科陕西西安 710004

收稿日期:2020-04-13 出版日期:2020-12-28 发布日期:2020-12-28
通讯作者: *苗棣, E-mail: corchorus@hotmail.com
作者简介:孙天祥(1991~ ),男,山东菏泽人,硕士,住院医师,主要从事牙周病的临床和基础研究。
基金资助:
国家自然科学基金青年科学基金项目(编号:81700986)

Impact of High Glucose on Porphyromonas Gingivalis LPS induced Inflammation of Periodontal Ligament Cells

SUN Tianxiang^1,2, MIAO Di^1,2*, WANG Baoyan², CHEN Yue²

1. Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, Hospital of Stomatology, Xi'an Jiaotong University, Xi'an 610041, China;
2. Department of Periodontics and Oral Mucosa Diseases, Hospital of Stomatology, Xi'an Jiaotong University, Xi'an 610041, China

Received:2020-04-13 Online:2020-12-28 Published:2020-12-28

摘要/Abstract

摘要： 目的:观察高糖对牙龈卟啉单胞菌(Porphyromonas gingivalis,P.gingivalis)重要毒力因子脂多糖(lipopolysaccharide,LPS)引发宿主牙周膜细胞释放炎症细胞因子和对核因子-κB (Nuclear factor-κB,NF-κB)表达、活化的影响。方法:人牙周膜细胞(human periodontal ligament cells,hPDLCs)培养于葡萄糖生理浓度中作为对照组,P.gingivalis LPS (1 μg/mL)、高糖(25 mmol/L)、高糖+P.gingivalis LPS刺激细胞6 h和12 h后,酶联免疫吸附法(enzyme linked immunosorbent assay,ELISA)检测上清液中白细胞介素-1β(Interleukin-1β,IL-1β)和肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)的释放,实时荧光定量PCR(quantitative real time PCR, qRT-PCR)和Western-blot检测NF-κB p65和磷酸化NF-κB p65(phospho-NF-κB p65, p-NF-κB p65)在hPDLCs中的表达水平。结果:高糖和P.gingivalis LPS可分别促进hPDLCs表达IL-lβ和TNF-α、促进NF-κB p65表达和活化,高糖和P.gingivalis LPS的共刺激可以增强这一促进作用。结论:高糖可能通过对NF-κB的活化增强P.gingivalis LPS致hPDLCs的炎症作用。

关键词: 牙龈卟啉单胞菌, 脂多糖, 高糖, 牙周炎, 糖尿病

Abstract: Objective: To investigate the impact of high glucose on inflammatory mediators release and NF-κB expression and on activation of human periodontal ligament cells induced by P.gingivalis lipopolysaccharide. Methods: Primary cultured hPDLCs were challenged in P.gingivalis LPS (1μg/mL), high glucose (25mmol/L), and high glucose+P.gingivalis LPS for 6 and 12 hours. The hPDLCs were cultured in physical concentration of glucose as control. The expression of TNF-α and IL-1β were detected by ELISA. The expression of NF-κB p65 and p-NF-κB p65 were measured by qRT-PCR and western-blot. Results: High glucose and P.gingivalis LPS could promote the expression of inflammatory cytokines TNF-α and IL-1β, and promote the expression and activation of NF-κB p65 in hPDLCs. The co-stimulation of high glucose and P.gingivalis LPS could enhance the expression of inflammatory cytokines and activation of NF-κB p65. Conclusion: High glucose may enhance the inflammation induced by P. gingivalis LPS through activation of NF-κB.

Key words: Porphyromonas gingivalis, lipopolysaccharide, high glucose, periodontitis, diabetes

孙天祥, 苗棣, 王宝彦, 陈悦. 高糖对牙龈卟啉单胞菌LPS引发牙周膜细胞炎症反应的影响[J]. 口腔医学研究, 2020, 36(12): 1123-1127.

SUN Tianxiang, MIAO Di, WANG Baoyan, CHEN Yue. Impact of High Glucose on Porphyromonas Gingivalis LPS induced Inflammation of Periodontal Ligament Cells[J]. Journal of Oral Science Research, 2020, 36(12): 1123-1127.

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高糖对牙龈卟啉单胞菌LPS引发牙周膜细胞炎症反应的影响

Impact of High Glucose on Porphyromonas Gingivalis LPS induced Inflammation of Periodontal Ligament Cells

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