铜掺入型钛合金对破骨细胞分化影响的体外研究

doi:10.13701/j.cnki.kqyxyj.2021.04.020

口腔医学研究 ›› 2021, Vol. 37 ›› Issue (4): 371-374.DOI: 10.13701/j.cnki.kqyxyj.2021.04.020

铜掺入型钛合金对破骨细胞分化影响的体外研究

许雄程^1,2, 卓瑾^1,2, 徐燕梅^1,2, 骆凯^1,2, 陈超^2*, 钟泉^2*

1.福建省口腔疾病研究重点实验室,福建省口腔生物材料工程技术研究中心,福建省高校口腔医学重点实验室,福建医科大学口腔医学院/附属口腔医院福建福州 350002;
2.福建医科大学口腔医学研究院,福建医科大学口腔组织工程研究中心,福建医科大学口腔医学院/附属口腔医院福建福州 350002

收稿日期:2020-12-16 发布日期:2021-04-15
通讯作者: *陈超,E-mail:dentcc@163.com;钟泉,E-mail:scott05@126.com
作者简介:许雄程(1991~ ),男,福建泉州人,博士在读,主要从事牙周病的临床及基础研究。
基金资助:
国家自然科学基金(编号:81870766)福建省自然科学基金项目(编号:2017J01805)福建省发改委第三批省级预算内投资计划项目福建医科大学启航基金项目(编号:2019QH2041)

Copper-doped Titanium Alloy Inhibited RANKL-induced Osteoclasts Differentiation in Vitro

XU Xiongcheng^1,2, ZHUO Jin^1,2, XU Yanmei ^1,2, LUO Kai^1,2, CHEN Chao^2*, ZHONG Quan^2*

1. Fujian Key Laboratory of Oral Diseases & Fujian Provincial Engineering Research Center of Oral Biomaterial & Stomatological Key laboratory of Fujian College and University, School and Hospital of Stomatology, Fujian Medical University, Fuzhou 350002, China;
2. Institute of Stomatology & Laboratory of Oral Tissue Engineering, School and Hospital of Stomatology, Fujian Medical University, Fuzhou 350002, China

Received:2020-12-16 Published:2021-04-15

摘要/Abstract

摘要： 目的: 研究铜掺入型钛合金对破骨细胞分化的影响。方法: 通过激光熔化技术制备掺铜的钛合金。使用RANKL诱导小鼠单核细胞分化,制备破骨细胞。将掺铜的钛合金与破骨细胞共培养,通过观察细胞形态、记录破骨细胞数目以及破骨相关基因的表达,分析掺铜的钛合金对破骨细胞分化相关基因表达的影响。结果: 小鼠单核细胞与RANKL共培养后可分化成破骨细胞。掺铜的钛合金能够抑制单核细胞向破骨细胞分化,同时还能降低破骨分化相关基因CTSK、TRAP、NFATc1和NF-κB的表达。 结论: 掺铜的钛合金可抑制破骨细胞的活性,有望应用于骨再生手术中个性化钛网的制备。

关键词: 钛合金, 铜, 破骨分化, 支架材料

Abstract: Objective: To study the effect of copper-doped titanium alloys on osteoclasts differentiation. Methods: Copper-doped titanium alloys were prepared by using the technique of selective laser melting (SLM). Mouse monocytes (RAW264.7) were induced to differentiate to osteoclasts by RANKL. Copper-doped titanium alloys was co-cultured with osteoclasts. The morphology of osteoclasts was observed under a fluorescence microscope. The number of osteoclasts was recorded, and the expression of genes related to osteoclast differentiation was detected by real-time fluorescence quantitative PCR. Results: Induced by RANKL, mouse monocytes could differentiate into osteoclasts in vitro. Copper-doped titanium alloys could not only inhibit the differentiation of monocytes (RAW264.7) which generated osteoclasts, but also inhibit the expression of osteoclast-related genes CTSK, TRAP, NFATc1, and NF-κB. Conclusion: Copper-doped titanium alloys can inhibit the activity of osteoclasts and is expected to be used in the preparation of titanium mesh which has potential application value for alveolar bone regeneration.

Key words: titanium alloy, copper, osteoclast differentiation, scaffold

许雄程, 卓瑾, 徐燕梅, 骆凯, 陈超, 钟泉. 铜掺入型钛合金对破骨细胞分化影响的体外研究[J]. 口腔医学研究, 2021, 37(4): 371-374.

XU Xiongcheng, ZHUO Jin, XU Yanmei , LUO Kai, CHEN Chao, ZHONG Quan. Copper-doped Titanium Alloy Inhibited RANKL-induced Osteoclasts Differentiation in Vitro[J]. Journal of Oral Science Research, 2021, 37(4): 371-374.

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铜掺入型钛合金对破骨细胞分化影响的体外研究

Copper-doped Titanium Alloy Inhibited RANKL-induced Osteoclasts Differentiation in Vitro

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