3D干细胞培养在牙髓再生研究中的应用

doi:10.13701/j.cnki.kqyxyj.2021.11.001

摘要/Abstract

摘要： 3D细胞培养是一种模拟人体内细胞生长环境的体外培养方式,使细胞保持原有的立体形态,有利于细胞内基因的表达和信号转导。3D干细胞培养作为其中一个重要的研究方向,主要应用于器官体外培养和组织再生领域。在牙髓再生研究中,干细胞经2D培养后,存在细胞接触抑制、基因表达受限以及部分生物学功能缺失的局限性,而3D培养可增强干细胞自我更新和特异性分化的能力,再生神经血管化的牙髓-牙本质复合体,再生效果明显优于2D培养。本文将介绍3D细胞培养的研究现状,总结分析3D干细胞培养在牙髓再生研究中的优势、应用及展望,为牙髓再生中干细胞培养条件和再生效果的优化提供理论依据。

关键词: 3D细胞培养, 牙髓, 牙髓再生, 干细胞

Abstract: 3D (Three-dimensional) culture of cells is a culture method in vitro. The growth and original 3D shape of cells in human body are simulated by this method which is beneficial to intracellular gene expression and signal transduction. As one of the important research directions, 3D culture of stem cells was mainly used in the field of organ culture and tissue regeneration. In the study of dental pulp regeneration, there are some limitations of cell contact inhibition, gene expression, and loss of biological functions in 2D (two-dimensional) culture of stem cells. However, the ability of self-renewal and specific differentiation of stem cells are enhanced by 3D culture and the regeneration effect of pulp-dentin complex with neurovascularization is significantly better than 2D monolayer culture. Therefore, in this review, the research status of 3D culture of cells are discussed. The advantage, application and prospect of 3D culture of stem cells in the research of dental pulp regeneration are summarized and analyzed. It is aimed to provide a theoretical basis for the optimization of culture conditions of stem cells and regeneration effect in dental pulp regeneration.

Key words: 3D culture, pulp, dental pulp regeneration, stem cells

牛卫东, 刘润园. 3D干细胞培养在牙髓再生研究中的应用[J]. 口腔医学研究, 2021, 37(11): 961-965.

NIU Weidong, LIU Runyuan. Applications Progress of 3D Culture of Stem Cells in the Research of Dental Pulp Regeneration[J]. Journal of Oral Science Research, 2021, 37(11): 961-965.

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