层粘连蛋白、整合素α6β1、黏着斑激酶在主导管结扎诱导大鼠腮腺萎缩过程中的表达及意义

doi:10.13701/j.cnki.kqyxyj.2021.12.013

摘要/Abstract

摘要： 目的:研究腮腺主导管结扎后层粘连蛋白、整合素α6β1、黏着斑激酶在腺体萎缩过程中的表达变化,初步探寻腮腺组织萎缩的信号机制。方法:对54只Wistar雌性大鼠建立左侧腮腺主导管结扎动物模型,分别于结扎后0(对照组)、1、3、5、7、10、14、21、30 d获取大鼠新鲜腮腺组织(n=6)。HE染色观察萎缩腮腺的组织学改变,免疫组织化学法、实时荧光定量PCR检测层粘连蛋白、整合素α6β1、黏着斑激酶在萎缩腮腺组织中的分布以及表达量变化。结果:主导管结扎后,腮腺逐渐萎缩。层粘连蛋白、整合素α6β1表达量在导管结扎后前3 d内均逐步下调,在结扎第3天表达量达到最低值(P<0.05),且两者在腺泡细胞基底部染色的完整性受到破坏;而后表达量连续升高到第14天到达峰值(P<0.05);随后缓慢降低(P<0.05)。黏着斑激酶表达量在腮腺萎缩过程中持续减低(P<0.05)。结论:层粘连蛋白/整合素α6β1/黏着斑激酶可作为腮腺萎缩过程中信号传导通路而发挥作用,该通路能够对导管结扎产生的机械力刺激作出反应并将机械力信号传递到细胞内,参与调控腮腺组织萎缩进程。

关键词: 腮腺, 信号传导, 细胞凋亡, 层粘连蛋白, 整合素类, 黏着斑激酶

Abstract: Objective: To study the expression of laminin, integrin α6β1, and focal adhesion kinase in parotid gland atrophy process in rats, and to explore the signaling mechanism of parotid gland atrophy. Methods: A ligation model of left parotid duct was established in 54 rats, and fresh parotid tissue was obtained at 0 (control group), 1, 3, 5, 7, 10, 14, 21, and 30 days after ligation (n=6). The histological changes of parotid gland were observed using hematoxylin eosin staining (HE), and the localization and expression of laminin, integrin α6β1, and focal adhesion kinase were detected by immunohistochemistry and quantitative real-time PCR. Results: Parotid gland gradually atrophied after duct ligation. The expression of laminin and integrin α6β1 decreased during the first 3 days after duct ligation, and reached the lowest value on the day 3 (P<0.05), and the integrity of distribution of laminin and integrin α6β1 in the base of acinar cells was damaged; then the expression of laminin and integrin α6β1 gradually raised and reached the peak on the 14th day, and then decreases (P<0.05). The focal adhesion kinase decreases continuously during parotid atrophy (P<0.05). Conclusion: Laminin/integrin α6β1/focal adhesion kinase may act as a signaling pathway during parotid gland atrophy, which can respond to mechanical stimulation generated by duct ligation and transmit mechanical signals into cells, and participate in regulating the parotid atrophy process.

Key words: parotid gland, signal transduction, apoptosis, laminin, integrins, focal adhesion kinase

于成龙, 杨勇, 薛博元, 李建卫, 高红, 宋娇娇, 刘郭琦, 左金华. 层粘连蛋白、整合素α6β1、黏着斑激酶在主导管结扎诱导大鼠腮腺萎缩过程中的表达及意义[J]. 口腔医学研究, 2021, 37(12): 1119-1124.

YU Chenglong, YANG Yong, XUE Boyuan, LI Jianwei, GAO Hong, SONG Jiaojiao, LIU Guoqi, ZUO Jinhua. Significance of Laminin, Integrin α6β1, and Focal Adhesion Kinase in Parotid Atrophy Induced by Main Duct Ligation in Rats[J]. Journal of Oral Science Research, 2021, 37(12): 1119-1124.

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