口腔医学研究

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (12): 1179-1183.

• 基础研究论著 • 上一篇    下一篇

重组人β防御素3对牙龈卟啉单胞菌脂多糖致炎作用的影响

吕晶露,卞添颖,李丽丽,崔迪,张婷,雷浪,闫福华*   

  1. 南京大学医学院附属口腔医院牙周科 江苏 南京 210008
  • 收稿日期:2015-09-15 出版日期:2015-12-28 发布日期:2016-03-21
  • 通讯作者: 闫福华,E-mail:fhyan2005@126.com
  • 作者简介:吕晶露(1989~),女,江苏省南京市人,硕士在读,主要从事牙周病和全身疾病关系的研究工作。
  • 基金资助:
    国家自然科学基金项目(编号:81371152)
    江苏省自然科学基金项目(编号:BK20131079)
    江苏省临床科技专项(编号:BL2013002)

Effects of Recombinant Human Beta-defensin-3 on Inflammation Induced by Porphyromanas Gingivilis Lipopolysaccharide.

LV Jing-lu, BIAN Tian-ying , LI Li-li, CUI Di, ZHANG Ting, LEI Lang, YAN Fu-hua.   

  1. Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing 210008, China
  • Received:2015-09-15 Online:2015-12-28 Published:2016-03-21

PDF (PC)

179

摘要： 目的:以牙龈卟啉单胞菌脂多糖(P.g-LPS)诱发载脂蛋白E基因敲除(ApoE-/-)小鼠和RAW264.7小鼠单核巨噬细胞的急性炎症反应,并应用重组人β防御素3(rhBD3),观察其对炎症的干预效果。方法:20周龄雄性ApoE-/-小鼠随机均分为PBS对照组、P.g-LPS组和P.g-LPS+rhBD3组,分别经腹腔注射给药2h后,检测血清中不同炎症指标(MCP-1、TNF-α、IL-6、IL-1β和NO)的水平。同时,以rhBD3干预P.g-LPS对RAW264.7细胞的致炎作用,分别检测培养上清和细胞中炎症指标的水平及其mRNA的相对表达量。结果:经P.g-LPS刺激后,ApoE-/-小鼠血清MCP-1、TNF-α、IL-6和NO的水平较对照组显著上调;而同时应用rhBD3能明显降低MCP-1、TNF-α和NO表达量。P.g-LPS能显著上调RAW264.7细胞培养上清中TNF-α和NO的水平以及细胞中TNF-α和IL-6的mRNA相对表达量,rhBD3对此具有抑制作用。结论:rhBD3对P.g-LPS在体内、外诱导的急性炎症具有一定的抑制效应,可能在牙周炎与高脂血症的相互作用中发挥免疫调节作用。

关键词: 重组人β防御素3(rhBD3), 牙龈卟啉单胞菌脂多糖(P.g-LPS), 抗炎

Abstract: Objective: To investigate the effects of recombinant human beta-defensin-3 (rhBD3) on acute inflammation induced by Porphyromanas gingivilis lipopolysaccharide (P.g-LPS) in apolipoprotein E knockout (ApoE-/-) mice and murine RAW264.7 macrophages. Methods: Male ApoE-/- mice (20 weeks old) were randomly divided into PBS control group, P.g-LPS group and P.g-LPS+rhBD3 group, and were stimulated by intraperitoneal injection. Serum inflammation markers (MCP-1, TNF-α, IL-6, IL-1β and NO) secretion levels were measured 2 hours later. The effects of rhBD3 on inflammation induced by P.g-LPS in RAW264.7 cells were also evaluated by detecting the secretion levels and mRNA relative expression levels of inflammation markers in culture supernatant and cells, respectively. Results: Serum MCP-1, TNF-α, IL-6 and NO secretion levels in P.g-LPS group of ApoE-/- mice were significantly upregulated, while the levels of MCP-1, TNF-α and NO decreased significantly in P.g-LPS+rhBD3 group. The increased levels of TNF-α and NO in culture supernatant and mRNA relative expression levels of TNF-α and IL-6 in RWA264.7 cells stimulated by P.g-LPS could be downregulated by rhBD3. Conclusion: rhBD3 has suppressive effect on acute inflammation induced by P.g-LPS in vivo and in vitro. It may play a modulating role in the interaction between periodontitis and hyperlipidemia.

中图分类号: