g-C3N4基复合材料在牙周炎中的光催化/光热抗菌治疗研究

doi:10.13701/j.cnki.kqyxyj.2025.09.007

摘要/Abstract

摘要： 目的: 研究单原子Cu掺杂石墨相氮化碳(graphitic carbon nitride, g-C₃N₄)结合硫化铋(Bi₂S₃)的复合材料对牙周炎中牙龈卟啉单胞菌(Porphyromonas gingivalis, P. gingivalis)的光热/光催化协同抗菌作用。方法: 水热法合成g-C₃N₄(简写为CN)纳米片和Bi₂S₃(简写为BiS),CN接枝Cu²⁺得到CuCN,基于静电吸附法合成纳米复合材料CuCN@BiS,对纳米材料的形貌、电位及晶体结构等进行表征;利用2',7'-二氯二氢荧光素二乙酸酯(2',7'-dichlorodihydrofluorescein diacetate, DCFH-DA)荧光法测试复合材料在体外产生活性氧(reactive oxygen species, ROS)效率;采用808 nm近红外光照射CuCN@BiS,评估光热性能;通过菌落计数及结晶紫染色评估CuCN@BiS对P. gingivalis的抗菌效率及对生物膜的清除效果;利用Cell Counting Kit-8(CCK-8)实验评估CuCN@BiS的生物相容性。结果: 成功构建复合材料CuCN@BiS,测试其在近红外光照射下具有良好的光催化和光热性能。CuCN@BiS对P. gingivalis的杀伤作用和对生物膜的清除效果具有浓度依赖性,浓度为0.5 mg/mL时展现出优异的抗菌效果(98.36%)及抗生物膜效率(92.84%)。同时,在近红外光照射下,进入细菌内的ROS量与 CuCN@BiS浓度呈正相关。0.5 mg/mL的CuCN@BiS的细胞相对活性在85%以上,生物相容性良好。结论: 应用CuCN@BiS进行抗菌的光催化协同光热治疗可以有效破坏P. gingivalis及其生物膜,为牙周炎的治疗提供参考。

关键词: 纳米复合材料, 光催化, 光热治疗, 牙龈卟啉单胞菌

Abstract: Objective: To investigate the photothermal/photocatalytic synergistic antimicrobial effect of monatomic Cu-doped graphitic carbon nitride (g-C₃N₄) combined with bismuth sulfide (Bi₂S₃) composites against porphyromonas gingivalis (P.g) in periodontitis. Methods: g-C₃N₄ (abbreviated as CN) nanosheets and Bi₂S₃ (abbreviated as BiS) were synthesized by hydrothermal method, CN was grafted to Cu²⁺ to obtain CuCN and the composite material CuCN@BiS was synthesized based on electrostatic adsorption method. Nanomaterials was characterized in terms of morphology, potential, and crystal structure. Composites were tested for reactive oxygen species production in vitro using 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA) fluorescence. The 808 nm near infrared (NIR) light exciter were used to evaluate the photothermal properties of CuCN@BiS. The antimicrobial efficiency of the composite CuCN@BiS against P.g by colony counting and the removal of biofilm were assessed by crystal violet staining. The biocompatibility of CuCN@BiS was observed using the cell counting kit-8 (CCK-8) experiment. Results: The composite CuCN@BiS was successfully constructed and had good photothermal properties under NIR light irradiation. The antimicrobial effect on P.g and the scavenging effect on biofilm of CuCN@BiS were concentration-dependent. When the concentration was 0.5 mg/mL, CuCN@BiS demonstrated excellent antimicrobial (98.36%) and anti-biofilm efficiency (92.84%). Meanwhile, under NIR irradiation, the amount of ROS entering the bacteria was positively correlated with the concentration of CuCN@BiS. The relative activity of the cells in 0.5 mg/mL of CuCN@BiS was above 85%, which demonstrated good biocompatibility. Conclusion: Application of CuCN@BiS for photocatalytic/photothermal antimicrobial treatment could effectively destroy P.g and its biofilm, providing references for the treatment of periodontitis.

Key words: nanocomposites, photocatalytic therapy, photothermal therapy, Porphyromonas gingivalis

顾菀蓉, 冉蓓, 赵奕雯, 廖金凤. g-C₃N₄基复合材料在牙周炎中的光催化/光热抗菌治疗研究[J]. 口腔医学研究, 2025, 41(9): 773-780.

GU Wanrong, RAN Bei, ZHAO Yiwen, LIAO Jinfeng. Photocatalytic/photothermal Antimicrobial Treatment of g-C₃N₄-based Composites in Periodontitis[J]. Journal of Oral Science Research, 2025, 41(9): 773-780.

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g-C₃N₄基复合材料在牙周炎中的光催化/光热抗菌治疗研究

Photocatalytic/photothermal Antimicrobial Treatment of g-C₃N₄-based Composites in Periodontitis

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