大鼠单根牙位点保存的标准化影像学分析方法研究

doi:10.13701/j.cnki.kqyxyj.2025.10.007

口腔医学研究 ›› 2025, Vol. 41 ›› Issue (10): 875-882.DOI: 10.13701/j.cnki.kqyxyj.2025.10.007

大鼠单根牙位点保存的标准化影像学分析方法研究

杨志康¹, 李玲², 彭体武¹, 任光辉^1,3, 王新峦², 姜雨汐^1,4*, 柳忠豪^1,5*

1.滨州医学院口腔医学院山东烟台 264003;
2.中国科学院深圳先进技术研究院医工所转化中心广东深圳 518060;
3.滨州医学院附属烟台口腔医院咬合门诊山东烟台 264003;
4.深圳大学医学部口腔医学院广东深圳 518060;
5.滨州医学院附属烟台口腔医院种植门诊山东烟台 264003

收稿日期:2025-04-02 发布日期:2025-10-23
通讯作者: *姜雨汐,E-mail:andrea-jiang@szu.edu.cn; 柳忠豪,E-mail:dentlzh@163.com
作者简介:杨志康(2000~ ),女,山东东营人,硕士在读,研究方向:牙槽骨再生。
基金资助:
广东省基础与应用基础研究基金(编号:2021A1515110211) 广东省医学科学技术研究基金项目(编号:B2023072)

Study on Standardized Imaging Analysis Methods for Single-rooted Tooth Site Preservation in Rats

YANG Zhikang¹, LI Ling², PENG Tiwu¹, REN Guanghui^1,3, WANG Xinluan², JIANG Yuxi^1,4*, LIU Zhonghao^1,5*

1. School of Stomatology, Binzhou Medical University, Yantai 264003, China;
2. Medical Engineering Division, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518060, China;
3. Center of Occlusion, Yantai Stomatological Hospital Affiliated to Binzhou Medical University, Yantai 264003, China;
4. School of Dentistry, Shenzhen University Medical School, Shenzhen 518060, China;
5. Center of Implantology, Yantai Stomatological Hospital Affiliated to Binzhou Medical University, Yantai 264003, China

Received:2025-04-02 Published:2025-10-23

摘要/Abstract

摘要： 目的: 在微型计算机断层扫描(micro-computed tomography, Micro-CT)指导下,依据牙槽骨解剖特征建立大鼠单根牙牙槽骨缺损模型,确定兴趣区范围与定量指标,建立具有可重复性和可比性的大鼠单根牙牙槽骨缺损修复分析方法,并验证其可行性。方法: 选取5月龄雄性大鼠15只,随机分为3组:解剖分析组,用于通过Micro-CT确定缺损位置和尺寸;空白对照组(Control)和引导骨再生(guided bone regeneration, GBR)实验组用于验证模型和评价方法可行性。在上颌第一磨牙区制备直径2 mm×深度2 mm缺损,通过Micro-CT分析量化颊侧骨高度丢失(buccal bone height loss, BBHL)和缺损区域新生骨及骨微结构。结果: 成功建立了SD大鼠的标准牙槽骨缺损模型,未损伤相邻牙齿且缺损区域颊侧骨壁厚度为(0.36±0.06) mm。术后8周,GBR组较Control组中间点、最低点BBHL和骨小梁距离显著减少(P<0.05),骨体积分数显著增加(P<0.05),骨小梁厚度及数量也较Control组有所改善。结论: 在Micro-CT指导下建立了标准化的大鼠牙槽骨缺损模型用于模拟临床单根牙缺损以及缺损修复的评价方法,该方法具有可重复性,为牙槽嵴保存技术和骨再生材料开发提供了可靠的临床前评估工具。

关键词: 微型计算机断层扫描, 位点保存, 动物模型, 定量分析, 牙槽骨缺损

Abstract: Objective: To establish a standardized alveolar bone defect model in Sprague-Dawley (SD) rat single-rooted teeth guided by micro-computed tomography (Micro-CT) anatomical analysis, develop quantitative region-of-interest (ROI) parameters, and validate the analytical protocol's feasibility for bone regeneration assessment. Methods: Fifteen 5-month-old male SD rats were randomized into three groups: anatomical analysis group for micro-CT guided defect parameter determination, blank control (Control) and positive (guided bone regeneration, GBR) groups for verification. The standard defect (2 mm diameter × 2 mm depth) was created in the maxillary first molar. Micro-CT was used to analyze the quantified buccal bone height loss (BBHL) and regenerated bone parameters. Results: A standardized alveolar bone defect model was successfully established in SD rats, preserving intact adjacent teeth and a buccal bone wall thickness of (0.36±0.06) mm in the defect region, consistent with the morphological characteristics of single-rooted tooth defects in clinical. At 8 weeks post-operation, compared to the Control group, the GBR group demonstrated significant reductions in BBHL at both the midpoint and lowest point, as well as in trabecular separation (Tb.Sp) (P<0.05). Concurrently, the GBR group exhibited a significant increase in bone volume fraction (BV/TV) (P<0.05) and improvements in trabecular thickness (Tb.Th) and trabecular number (Tb.N). Conclusion: This micro-CT-guided protocol establishes a reproducible thin-wall alveolar defect model to mimic single-rooted teeth with standardized analytical parameters, providing a reliable preclinical evaluation tool for alveolar ridge preservation techniques and bone regeneration material development.

Key words: micro-CT, site preservation, animal model, quantitative analysis, alveolar bone defect

杨志康, 李玲, 彭体武, 任光辉, 王新峦, 姜雨汐, 柳忠豪. 大鼠单根牙位点保存的标准化影像学分析方法研究[J]. 口腔医学研究, 2025, 41(10): 875-882.

YANG Zhikang, LI Ling, PENG Tiwu, REN Guanghui, WANG Xinluan, JIANG Yuxi, LIU Zhonghao. Study on Standardized Imaging Analysis Methods for Single-rooted Tooth Site Preservation in Rats[J]. Journal of Oral Science Research, 2025, 41(10): 875-882.

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大鼠单根牙位点保存的标准化影像学分析方法研究

Study on Standardized Imaging Analysis Methods for Single-rooted Tooth Site Preservation in Rats

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