Journal of Oral Science Research

Journal of Oral Science Research ›› 2018, Vol. 34 ›› Issue (1): 27-31.DOI: 10.13701/j.cnki.kqyxyj.2018.01.007

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Effects of Lemon Essential Oil on Acid Production and Activity of Lactic Dehydrogenase in Streptococcus Mutans

SUN Yan-wei1, LIU Ya-li2, ZHAO Xiao-xue1, HOU Fei-fei1, ZHANG Xiang-yu1*   

  1. 1. School of Stomatology, Tianjing Medical University. Tianjing 300000, China;
    2. Department of Endodontics, Tanggu Stomatology Hospital. Tianjing 300450, China
  • Received:2017-09-15 Online:2018-01-28 Published:2018-01-26

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Abstract: Objective: To investigate the effects of LEO on the acid production and activity of lactate dehydrogenase (LDH) in Streptococcus mutans, and to provide a theoretical basis for the application of lemon essential oil (LEO) in oral cavity. Methods: According to the minimal inhibitory concentration (MIC) of LEO, the dilution of 1/8MIC, 1/4MIC, 1/2MIC of LEO and the control group (only contained medium) were prepared by using two-fold dilution method. Streptococcus mutans was added into each group and cultivated for 24h. △pH of medium in each group was determined. The method of oxidation of reduction coellzymeⅠand RT-PCR were respectively adopted to evaluate the activity of LDH and the expression of ldh gene related to acid production. Results: With increasing concentrations of LEO, a dose-dependent decline of △pH (1.423-0.067) and the activity of LDH (33.279-13.367 U/mg) was observed in the LEO group compared to the control group at sub-MIC levels (P<0.01). Compared to the control group, the expression of ldh gene significantly decreased (P<0.05). Conclusion: Sub-MICs of LEO inhibited the activity of LDH and reduced acid production by down-regulating the expression of ldh gene without affecting bacterial growth.

Key words: Lemon essential oil, Streptcoccus mutans, Acid production, Lactate dehydrogenase

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