Animal Experiment on Cooperation between Shh and IGF-1 in Promoting Mandibular Cartilage Overgrowth

doi:10.13701/j.cnki.kqyxyj.2018.08.016

Journal of Oral Science Research ›› 2018, Vol. 34 ›› Issue (8): 866-869.DOI: 10.13701/j.cnki.kqyxyj.2018.08.016

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Animal Experiment on Cooperation between Shh and IGF-1 in Promoting Mandibular Cartilage Overgrowth

CHEN Yu-xiang^1,2#, HUANG Qun^2#, ZHANG Wu-yang¹, MA Qin^1*, LONG Xing^3*

1. State Key Laboratory of Military Stomatology, School of Stomatology, The Fourth Military Medical University, Xi'an 710032, China;
2. Stomatology Center, Guangdong Women and Children Hospital, Guangzhou 511400, China;
3. Department of Oral and Maxillofacial Surgery, School of Stomatology, Wuhan University, Wuhan 430079, China

Received:2018-02-26 Online:2018-08-28 Published:2018-08-23

Abstract

Abstract: Objective: To investigate the combination effect of Shh and IGF-1 in promoting the mandible condylar cartilage overgrowth. Methods: Transgenic rats with IGF-1-over expression in condylar cartilage were constructed to allow the local administration of IGF-1 and its specific inhibitor NVP-AEW541, and Cyclopamine, the specific inhibitor of Shh. All rats were divided into five experiment groups by different stimulating additions :(1)Cyclopamine;(2)NVP-AEW541;(3)IGF-1;(4)Cyclopamine+ IGF-1;(5)physiological saline. Unilateral condyle was randomly chosen to make the intra-capsule injection, and then its size was compared to the control condyle. Results: Compared with control condyle, the sizes of the condyle which were local injected with NVP-AEW541, IGF-1, and Cyclopamine+ IGF-1 were significantly smaller (P<0.05). There was no difference on the size of the condyle between cyclopamine and physiological saline stimulation groups (P<0.05). Conclusion: IGF-1 promotes human TMJ cartilage overgrowth in the developing process of condylar hyperplasia.

Key words: Condylar hyperplasia, Sonic hedgehog, Insulin-like growth factor-1

CHEN Yu-xiang, HUANG Qun, ZHANG Wu-yang, MA Qin, LONG Xing. Animal Experiment on Cooperation between Shh and IGF-1 in Promoting Mandibular Cartilage Overgrowth[J]. Journal of Oral Science Research, 2018, 34(8): 866-869.

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Animal Experiment on Cooperation between Shh and IGF-1 in Promoting Mandibular Cartilage Overgrowth

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