Preparation and in vitro Osteogenesis of Exendin4-PEGDA/HA Mineralized Hydrogel

doi:10.13701/j.cnki.kqyxyj.2020.08.007

Abstract

Abstract: Objective: To prepare a high strength PEGDA/HA mineralized hydrogel loaded with Exendin4 to induce the differentiation of osteoblast. Methods: PEGDA hydrogel was prepared by photopolymerization, and PEGDA/HA mineralized hydrogel was prepared by in-situ precipitation method, and Exendin4 was loaded by gel adsorption. The effects of different ratios of calcium and phosphorus concentrations on the strength of PEGDA/HA hydrogel were detected. The drug loading, Exendin4 release profile, biocompatibility and osteogenic activity of Exendin4-PEGDA/HA were also detected. Results: The PEGDA/HA mineralized scaffold had loose porous structure and high mechanical strength, meanwhile showed good sustained release curve within 20 days. Cell experiments showed that the high strength hydrogel had good biocompatibility and high osteogenic activity. Conclusion: The high strength Exendin4-PEGDA/HA showed good osteogenic activity in bone tissue regeneration, and has potential as a biomaterial for bone tissue repair.

Key words: Exendin4, PEGDA, mineralized hydrogel, sustained release

WANG Yansong, LIU Jianlin, CHEN Xiaoxia, BAI Shuting. Preparation and in vitro Osteogenesis of Exendin4-PEGDA/HA Mineralized Hydrogel[J]. Journal of Oral Science Research, 2020, 36(8): 731-735.

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