Effects of Chewing on GAT-1 in Amygdala and Frontal Cortex of Stressed Rats

doi:10.13701/j.cnki.kqyxyj.2020.08.008

Abstract

Abstract: Objective: To investigate the effect of mastication on GABA reuptake in amygdala and frontal cortex of rats. Methods: 52 SD rats were divided into control group (CO group), stress group (ST group), and stress + chewing group (SC group). Except for CO group, rats in each group were placed in a fixed tube for 30 minutes. Meanwhile, the SC group was given a 5mm diameter stick for chewing, and those chewing for less than 15 minutes were discarded. The motor scores of each group were recorded in Open-Field test. At the end of the experiment, 18 rats (6 in each group) were randomly selected and killed immediately. Results: Compared with CO group, the OF value of ST group was significantly lower, and the expression of GAT-1 mRNA in amygdala and frontal cortex was significantly higher (P<0.05). The OF value of SC group was significantly higher than ST group, and the expression of GAT-1 mRNA in amygdala and frontal cortex was significantly lower (P<0.05). There was no significant difference between SC group and CO group (P>0.05). Conclusion: Mastication can relieve the stress by correcting the abnormal expression of GAT-1 in amygdala and frontal cortex.

Key words: mastication, γ-aminobutyric acid, γ-aminobutyric acid transporter-1, stress-attenuation effects

YANG Jiadi, BIAN Ce, WANG Xu, LI Hexiang. Effects of Chewing on GAT-1 in Amygdala and Frontal Cortex of Stressed Rats[J]. Journal of Oral Science Research, 2020, 36(8): 736-740.

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