Experimental Study on Nicotine Promoting Root Migration of Junctional Epithelium in Rats

doi:10.13701/j.cnki.kqyxyj.2021.10.006

Abstract

Abstract: Objective: To investigate the effect of nicotine on root migration of junctional epithelium (JE) in rats. Methods: Twenty healthy male Wistar rats of six weeks of age were randomly divided into experimental group (intraperitoneal injection of nicotine 0.73mg/kg daily) and control group (intraperitoneal injection of the same amount of physiological saline daily). Each group was divided into two subgroups of 30 days and 90 days. After being killed according to the experimental design, the mesiodistal paraffin sections of tooth-periodontal joint tissue in the right mandibular molar area were routinely made and stained with Masson. The length of first molar enamel cementum boundary to the root apex of JE was measured. Results: On the 30th day, there was a slight root migration of JE in the experimental group compared with the control group without statistical significance (P>0.05). The collagen under the epithelium of both 30-day groups of rats was dense and neatly arranged. While the JE in the control and experimental groups had root migration at 90 days, the migration length of the experimental group was significantly longer than that of the control group (P<0.01), and the collagen under the epithelium of the experimental group was sparser and slender than the control group. In the different time points, the length of JE root migration in the experimental group at 90 days was significantly longer than that in the experimental group at 30 days (P<0.01). Conclusion: JE of rats migrated apically in a time-dependent manner, which could be enhanced by the intraperitoneal injection of nicotine.

Key words: nicotine, junctional epithelium, collagen fibers, root migration

ZHONG Yueyue, MA Su, QIN Zegen, ZHOU Huiling, LIU Peihong, LI Yanwu. Experimental Study on Nicotine Promoting Root Migration of Junctional Epithelium in Rats[J]. Journal of Oral Science Research, 2021, 37(10): 890-893.

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