Effect of Twin Inclined Plane Appliance on Expression of Type Ⅱ Collagen and Vascular Endothelial Growth Factor in Condyle of Growing Rats

doi:10.13701/j.cnki.kqyxyj.2019.04.010

Journal of Oral Science Research ›› 2019, Vol. 35 ›› Issue (4): 346-350.DOI: 10.13701/j.cnki.kqyxyj.2019.04.010

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Effect of Twin Inclined Plane Appliance on Expression of Type Ⅱ Collagen and Vascular Endothelial Growth Factor in Condyle of Growing Rats

WANG Shuo¹, HUA Xian-ming^1,2*, SUN Yu-hong³, XU Yan¹, XIA Lu-lu¹

1. The State Key Laboratory Breeding Base of Basic Science of Stomatology and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, China;
2. Department of Orthodontics, Hospital of Stomatology, Wuhan University, Wuhan 430079, China;
3. Department of Orthodontics, Peking University Shenzhen Hospital, Shenzhen 518035, China

Received:2018-08-20 Online:2019-04-28 Published:2019-04-23

Abstract

Abstract: Objective: To investigate the effect of twin inclined plane appliance (TIPA) on the expression of type II collagen and vascular endothelial growth factor (VEGF) in condyle of growing rats. Methods: Male 6-week-old Wistar rats (n=48) were randomly divided into control group (n=16) and experimental group (n=32). Rats in experiment group wore TIPA, whereas for the control groups no appliances were fitted. After 3, 14, 30, and 60 days, the rats were euthanatized and samples were collected. Immuohistochemical method was used to localize and examine the type II collagen and VEGF in the posterior area of mandibular condylar cartilage (MCC). Results: The expression of type II collagen decreased significantly on day 30 and reached the lowest on day 60 in the experimental group (P<0.01). The expression of VEGF increased significantly on day 30 (P<0.05) and reached the peak on day 60 in the experimental group (P<0.01). Conclusion: Both type II collagen and VEGF take part in MCC remolding during the gradually induced backward movement of mandible induced by TIPA.

Key words: Twin inclined plane appliance, Type Ⅱ collagen, Vascular endothelial growth factor

WANG Shuo, HUA Xian-ming, SUN Yu-hong, XU Yan, XIA Lu-lu. Effect of Twin Inclined Plane Appliance on Expression of Type Ⅱ Collagen and Vascular Endothelial Growth Factor in Condyle of Growing Rats[J]. Journal of Oral Science Research, 2019, 35(4): 346-350.

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Effect of Twin Inclined Plane Appliance on Expression of Type Ⅱ Collagen and Vascular Endothelial Growth Factor in Condyle of Growing Rats

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