Distribution of Multinucleated Giant Cell and Osteopontin Expression in Different Implant Materials-bone Interface

doi:10.13701/j.cnki.kqyxyj.2017.11.003

Journal of Oral Science Research ›› 2017, Vol. 33 ›› Issue (11): 1143-1146.DOI: 10.13701/j.cnki.kqyxyj.2017.11.003

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Distribution of Multinucleated Giant Cell and Osteopontin Expression in Different Implant Materials-bone Interface

LIU Jie¹, ZHANG Jie-yi¹, ZHOU Xuan¹, ZENG Yue¹, WANG Min², XIA Hai-bin^2*

1. School of Stomatology, Wuhan University, Wuhan 430079, China;
2. Department of Oral Implantology, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, China.

Received:2017-06-02 Online:2017-11-20 Published:2017-11-29

Abstract

Abstract: Objective: To compare the distribution of multinucleated giant cells (MCG) and the expression of osteopontin (OPN) in bone-implant interface between medical pure titanium (Ti) and polymethylmethacrylate (PMMA). Methods: Thirty Ti and PMMA implants were implanted into the distal femoral condyle of 30 SD rats. The animals were sacrificed after 3, 7, 14, 21 and 28 days. The paraffin sections were prepared. The distribution of MCG in the implant-bone interface was observed by HE staining. The expression of OPN in the implant-bone interface was detected by immunohistochemistry. Results: HE staining showed that MCG could be observed in the implant-bone interface at all time points, and the peak appeared at the 3rd day. The number of MCG in PMMA group was significantly more than that of Ti group (P<0.05). The number of MCG decreased with time in both groups, and there was no statistical difference after 21 days and 28 days. The expression of OPN was peaked at 7 days, and the expression of OPN in Ti group was significantly stronger than that of PMMA group (P<0.05). With time, the expression of OPN was gradually weakened both in titanium group and PMMA group. Conclusion: The distribution of MCG and the expression of OPN may be related to different implant materials-bone interface.

Key words: Multinucleated giant cells, Osteopontin, Implant-bone interface

CLC Number:

R783

LIU Jie, ZHANG Jie-yi, ZHOU Xuan, ZENG Yue, WANG Min, XIA Hai-bin. Distribution of Multinucleated Giant Cell and Osteopontin Expression in Different Implant Materials-bone Interface[J]. Journal of Oral Science Research, 2017, 33(11): 1143-1146.

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Distribution of Multinucleated Giant Cell and Osteopontin Expression in Different Implant Materials-bone Interface

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