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Pathologic Changes of Synovial Chondromatosis of Temporomandibular Joint. LI Ying-jie, FENG Ya-ping, DENG Mo-hong, LONG Xing 2017, 33(11): 1135-1138.  DOI: 10.13701/j.cnki.kqyxyj.2017.11.001 Abstract ( 454 )   PDF (2017KB) ( 285 )   Objective: To investigate the histological features of synovial chondromatosis (SC) of temporomandibular joint (TMJ) and the key to promote pathogenic process. Methods: Loose bodies(LBs)and synovium specimens were treated with histological methods. Results: During Milgram phaseⅠ, cartilaginous nodules (CNs) with a length of 30μm formed in synovium's sublining layer rich in blood vessels. During Milgram phaseⅡ, CNs with a length of about 0.5mm detached from synovium and became LBs which were nurtured by synovial fluid. The length of LB could increase to 3 mm. During Milgram phase Ⅲ, bone trabecular replaced the majority of cartilaginous extracellular matrix in the LB with a length of 4.3mm. Conclusion: During Milgram phaseⅠ, angiogenesis guaranteed enough nutrients supply for CNs formation. During Milgram phaseⅡ, enough nutrients supply from synovial fluid facilitated further volume increase of LBs. During Milgram phase Ⅲ, LB suffered from ossification and structure changes in order for more nutrients permeation into deep area. Therefore, the need of CNs or LBs for nutrients promoted the pathogenic process of TMJSC. References | Related Articles | Metrics

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Effects of MicroRNA 34a-5p on Inflammation and Differentiation of Osteoblast WANG Gui-ling, YANG Di, GUO Jia-jie, YU Ya-qiong, QIU Li-hong 2017, 33(11): 1139-1142.  DOI: 10.13701/j.cnki.kqyxyj.2017.11.002 Abstract ( 436 )   PDF (1443KB) ( 203 )   Objective: To investigate the effect of microRNA 34a-5p (miR-34a-5p) on the inflammation and differentiation of osteoblasts. Methods: MG63 was transfected with miR-34a-5p mimics to ensure a higher expression than general negative control (NC). Expression of miR-34a-5p was detected after MG63 was stimulated by different concentrations of lipopolysaccharide (LPS) from porphyromonas endodontalis (P.e). Transfected cells were stimulated by 20 mg/L P.e-LPS for 24 h, and expression of IL-6 was detected. Expressions of osteogenic differentiation markers and Notch1 were detected after osteogenic induction for 48 h. Results: Expression of miR-34a-5p in MG63 was down-regulated by P.e-LPS with different concentrations(P<0.05). MiR-34a-5p inhibited the expression of IL-6 significantly both with and without the stimulation of P.e-LPS(P<0.01). Osteogenic makers in MG63 were promoted by miR-34a-5p(P <0.05)and Notch1 was down-regulated(P<0.05). Conclusion: MiR-34a-5p inhibits the inflammation and promotes the differentiation of osteoblast, and Notch1 may serve as a target during the process. References | Related Articles | Metrics

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Distribution of Multinucleated Giant Cell and Osteopontin Expression in Different Implant Materials-bone Interface LIU Jie, ZHANG Jie-yi, ZHOU Xuan, ZENG Yue, WANG Min, XIA Hai-bin 2017, 33(11): 1143-1146.  DOI: 10.13701/j.cnki.kqyxyj.2017.11.003 Abstract ( 227 )   PDF (1465KB) ( 173 )   Objective: To compare the distribution of multinucleated giant cells (MCG) and the expression of osteopontin (OPN) in bone-implant interface between medical pure titanium (Ti) and polymethylmethacrylate (PMMA). Methods: Thirty Ti and PMMA implants were implanted into the distal femoral condyle of 30 SD rats. The animals were sacrificed after 3, 7, 14, 21 and 28 days. The paraffin sections were prepared. The distribution of MCG in the implant-bone interface was observed by HE staining. The expression of OPN in the implant-bone interface was detected by immunohistochemistry. Results: HE staining showed that MCG could be observed in the implant-bone interface at all time points, and the peak appeared at the 3rd day. The number of MCG in PMMA group was significantly more than that of Ti group (P<0.05). The number of MCG decreased with time in both groups, and there was no statistical difference after 21 days and 28 days. The expression of OPN was peaked at 7 days, and the expression of OPN in Ti group was significantly stronger than that of PMMA group (P<0.05). With time, the expression of OPN was gradually weakened both in titanium group and PMMA group. Conclusion: The distribution of MCG and the expression of OPN may be related to different implant materials-bone interface. References | Related Articles | Metrics

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Effects of Autologous Concentrated Growth Factors Extract on MC3T3-E1 Cells ZHU Zhong-yan, ZHANG Lu, LIU Min 2017, 33(11): 1147-1150.  DOI: 10.13701/j.cnki.kqyxyj.2017.11.004 Abstract ( 239 )   PDF (1147KB) ( 228 )   Objective: To evaluate the effect of the concentrated growth factors (CGF) extract on the proliferation and differentiation of MC3T3-E1 osteoblasts. Methods: The experimental group was cultured with α-MEM containing CGF, while the control group was α-MEM. The proliferation of the cell was detected by CCK-8, the activity of alkaline phosphatase was detected by ALP, and the expressions of runt-related transcription factor-2 (Runx2) and osterix (Osx) genes were quantified by real-time PCR. Results: CCK-8 showed that the experimental groups with different concentrations of CGF had different proliferation and the 10% CGF group was higher than other groups. When 10% CGF was used as experimental concentration, the ALP activity of experimental group was higher than that of control group after 3, 5, and 7d. RT-PCR results showed that the expressions of Runx2 and Osx in experimental group significantly enhanced. Conclusion: The growth factors released by CGF can promote the proliferation and differentiation of MC3T3-E1 osteoblasts. References | Related Articles | Metrics

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Effect of N-acetyl Cysteine on Lipopolysaccharide Induced Expressions of Genes and Proteins in MC3T3-E1 Osteoblasts ZHANG Hui, LI Wang-yang, HUANG Ying, CAI Yue, GUO Ling 2017, 33(11): 1151-1155.  DOI: 10.13701/j.cnki.kqyxyj.2017.11.005 Abstract ( 206 )   PDF (1618KB) ( 208 )   Objective: To evaluate the effect of N-acetyl cysteine on Lipopolysaccharide induced expressions of genes and proteins in MC3T3-E1 osteoblasts. Methods: The osteoblasts were isolated from the calvarias of mice and cultured in a-MEM. Cells were stimulated with various concentrations of NAC or LPS and then the proliferation of the cells was observed at different time points (6h, 24h, 48h, and 72h) by CCK-8. The expression of ALP, BGP, NF-KB, and IL-6 were determined by qPCR and ELISA analyses. Results: The proliferation of the cells increased with the increasing concentrations of NAC. When the concentration of NAC was 1mmol/L, the proliferation of the cells reached the maximum. A higher concentration of NAC had toxic effects. The down-regulated gene and protein expressions of ALP and BGP induced by 10μg/ml LPS were promoted significantly after treated with 1mmol/L NAC. The up-regulated gene and protein expressions of IL-6 and NF-kB induced by 10μg/ml LPS were decreased significantly after treated with 1mmol/L NAC. Conclusion: Expressions of ALP and BGP induced by LPS were promoted significantly after treated with NAC. NAC could decrease the expression of IL-6 induced by LPS through the NF-kB signaling. References | Related Articles | Metrics

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FPS-ZM1 Rescues the Inflammatory Response of hPDLFs Caused by High Glucose via Inhibition of RAGE ZHAN Dan-ting, DING Nong-le, ZHANG Yun-shui, ZHANG Hui, GUO Ling 2017, 33(11): 1156-1160.  DOI: 10.13701/j.cnki.kqyxyj.2017.11.006 Abstract ( 270 )   PDF (1188KB) ( 226 )   Objective: To observe the effect of FPS-ZM1 on IL-6 and TNF-α expression of human periodontal ligament fibroblasts (hPDLFs) cultured in high glucose. Methods: hPDLFs were primarily cultured and exposed to either low glucose (5.5mmol/L) or high glucose (25mmol/L). Under high glucose, the expression of RAGE was measured by Western-blot. The gene and protein expressions of IL-6 and TNF-α were measured by RT-PCR and ELISA under high glucose with various concentration of FPS-ZM1. Results: High glucose could enhance RAGE expression and the inflammatory response of hPDLFs, which was inverted by RAGE inhibitor FPS-ZM1. Conclusion: FPS-ZM1 can rescue the inflammatory response of hPLDFs caused by high glucose. References | Related Articles | Metrics

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Detection of Th17 and Related Factors in Gingival Crevicular Fluid of Patients with Different Periodontal Inflammatory Status YANG Dong, LI Hong-mei, LIU Lin-hua, DONG Wei-li 2017, 33(11): 1161-1164.  DOI: 10.13701/j.cnki.kqyxyj.2017.11.007 Abstract ( 212 )   PDF (859KB) ( 173 )   Objective: To study the correlation of Th17 and related factors in the gingival crevicular fluid (GCF) of the patients with different periodontal inflammatory status. Methods: Forty-six patients were divided into four groups: periodontitis active site group, non-active site group, gingivitis group, and healthy group. IL-17, RORC2, IFN-γ, and IL-4 in GCF were detected by enzyme-linked immunosorbent assay (ELISA). Results: The expression trend of IL-17 and RORC2 were similar. The levels of IL-17 and RORC2 in active site group were significantly higher than those of other three groups, and the levels of non-active site group were significantly higher than that of healthy group (P<0.05). The expression of IFN-γ in healthy group was significantly lower than those of other three groups (P<0.05). In active periodontal site group, the expression level of IL-4 was significantly lower than those of other three groups (P<0.05). IL-17 level was positively correlated with RORC2, and negatively correlated with IL-4 (P<0.05). The levels of IL-17, RORC2 and IFN-γ were positively correlated with those of BI, PD and AL. The level of IL-4 was negatively correlated with those of BI, PD and AL (P<0.05). Conclusion: The expression of Th17 was strong in active site group, and it increased with the aggravation of attachment loss. IFN-γ might be involved in the transformation of gingival inflammation and non-inflammation status. References | Related Articles | Metrics

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Changes of Microarchitecture of Alveolar Bone in Experimental Periodontitis in Rats CHENG Jing-yi, ZHANG Yun, CHENG Cai-yun, CHENG Lan, ZHANG Xiu-yin 2017, 33(11): 1165-1168.  DOI: 10.13701/j.cnki.kqyxyj.2017.11.008 Abstract ( 262 )   PDF (1083KB) ( 282 )   Objective: To study the changes of the microarchitecture of alveolar bone in the experimental periodontitis in rats. Methods: Fourteen 3-month-old Sprague-Dawley rats were used to establish periodontitis model by ligaturing the left maxillary 1st molar (PER), and the right maxillary 1st molar was used as control (CON). The rats were sacrificed respectively 4 weeks and 8 weeks later. The maxilla was analyzed by Micro-CT scanning. All statistical analysis was performed with SPSS. Results: The probing depth in PER group increased in comparison to CON group at both 4 and 8 weeks. The alveolar bone of CON group was compacted with plate-like trabecular, while the alveolar bone of PER group become porotic with rod-like trabecular. When compared with corresponding CON group, BMD, BV/TV, and Tb.Th. of alveolar bone significantly decreased in PER group at both 4 and 8 weeks. BMD, BV/TV, and Tb.Th. in 8-week PER group decreased compared with 4-week PER group. Tb.N and Tb.Sp. in 8-week PER group increased in compared with both CON group and 4-week PER group. Conclusion: Periodontitis could induce bone loss and deterioration of trabeculae of alveolar bone. The deterioration might be aggravated by the extended duration. References | Related Articles | Metrics

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Effects of Zoledronic Acid on Biological Characteristics of Gingival Fibroblasts DONG Wen-jie, WANG Yu-kun, YUE Wen-jiang 2017, 33(11): 1169-1172.  DOI: 10.13701/j.cnki.kqyxyj.2017.11.009 Abstract ( 223 )   PDF (1135KB) ( 191 )   Objective: To investigate the effect and mechanism of zoledronic acid on proliferation, apoptosis, and differentiation of gingival fibroblasts. Methods: Primary human gingival fibroblasts (HGFs) isolated from the gingival tissue were treated with 0.5, 1, 5, and 10 μmol/L zoledronic acid for 24, 48, and 72h. Cell proliferation was detected by CCK8 assay. Cell apoptosis was detected by flow cytometry in 10 μmol/L zoledronic acid treated HGFs cells for 72h. Alkaline phosphatase (ALP) activity was detected by ALP kit, the expression of Runx2 and OPN protein were detected by Western blot as well as p65 and IκBα protein after treated with 10 μmol/L zoledronic acid for 72h. Results: Zoledronic acid inhibited proliferation of HGFs in a dose dependent manner (P<0.01). Apoptosis rate of cells in zoledronic acid was significantly higher than that of control group. ALP activity and the expression of Runx2 and OPN protein were significantly higher than those of control group, whereas the protein expressions of p65 and IκBα were significantly lower than the control group (P<0.01). Conclusion: Zoledronic acid can inhibit the proliferation but promote the apoptosis and differentiation of HGFs by downregulating NF-κB signaling pathway. References | Related Articles | Metrics

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Comparative Study of Periodontal Health in HIV Patients DU Fei, GE Liu-ying, HE Rui, LI Chong-xi, Zeng Shui-qin, DUAN Kai-wen 2017, 33(11): 1173-1176.  DOI: 10.13701/j.cnki.kqyxyj.2017.11.010 Abstract ( 196 )   PDF (800KB) ( 263 )   Objective: To explore the periodontal conditions of HIV patients, and to evaluate their periodontal conditions and risk factors. Methods: The periodontal health care knowledge and behaviors were analyzed, and the periodontal health status in 216 patients with HIV and 76 patients without HIV was checked, respectively. Results: There was no significant differences among the HIV patients with or without highly active antiretroviral therapy and the control group in oral health care and oral treatment methods. But the HIV patients were less often changing tooth brush, which had significant difference among different groups (P<0.01). In addition, HIV patients used tooth floss or toothpicks more frequent than control group, which had significant difference among different groups (P<0.05). In the examination of periodontal indexes, there were significant difference in BOP results and probing depth between the HIV+ patients and control group (P<0.05), but there was no correlation between the CD4+ T cell counts and CAL. Conclusion: In this questionnaire, the oral health cognition of the HIV patients was similar to general population, only a few of HIV patients had periodontal health worse than those of non HIV patients. References | Related Articles | Metrics

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Short-term Curative Effect of Minocycline Gel and Hyaluronic Acid Gel as Adjunctive Treatment on Chronic Periodontitis CHEN Fan, Zhang Yong 2017, 33(11): 1177-1180.  DOI: 10.13701/j.cnki.kqyxyj.2017.11.011 Abstract ( 237 )   PDF (1018KB) ( 406 )   Objective: To compare the curative effects of two different gels as adjunctive treatment on chronic periodontitis. Methods: One hundred and five patients with chronic periodontitis in the Department of Stomatology of our hospital from June, 2012 to November, 2016 were randomly divided into three groups based on therapeutic prescription: group A (n=35, hyaluronic acid gel), group B (n=35, minocycline gel), and group C (n=35, SRP). After 3 months, the periodontal indexes, curative effect, and adverse drug reactions among three groups were compared. Results: Periodontal indexes in group A and B were significantly lower than that of group C (P<0.05). The PLI and percentage of spirochetes in sub-gingival plaque of group B at the 4th week after treatment were obviously lower than those of other two groups (P<0.05). The curative rates of group A and B were obviously higher than that of C group (P<0.05). The incidence of adverse drug reactions in group A was obviously lower than that of group B (P<0.05). Conclusion: These two different gels had similar curative effect as adjunctive treatment on chronic periodontitis. References | Related Articles | Metrics

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Effect of TRAF6 Knockdown on Osteogenic Differentiation of Human Periodontal Ligament Cell Treated with LPS CHEN Qing-yong, LI Xia, ZHANG Fang, WANG Jin-hua 2017, 33(11): 1181-1184.  DOI: 10.13701/j.cnki.kqyxyj.2017.11.012 Abstract ( 280 )   PDF (1029KB) ( 370 )   Objective: To observe the effect of tumor necrosis factor receptor-related factor 6 (TRAF6) knockdown on osteogenic differentiation of human periodontal ligament cell (hPDLC) stimulated by LPS. Methods: The TRAF6 siRNA and control siRNA were transiently transfected into hPDLC. The cells were stimulated with 10μg/ml LPS. The silence efficiency of TRAF6 was detected by RT-PCR and Western blot. The expression of ALP was detected by alkaline phosphatase assay kit. The expression of Runx-2 and type I collagen (Col-I) gene was detected by RT-PCR. Results: The expression of TRAF6 mRNA and protein in TRAF6 siRNA group was significantly decreased (P<0.001). The expression of ALP in LPS-stimulated group was significantly lower than that in non-LPS-stimulated group (P<0.05). The expression of ALP, Runx-2, and Col-I mRNA in TRAF6 siRNA was significantly higher than that in the other three groups (P<0.05). Conclusion: TRAF6 knockdown can alleviate the inhibitory effect of LPS on osteogenesis differentiation of hPDLC, which indicates that TRAF6 may affect the development of periodontitis and may be a potential therapeutic target for periodontitis. References | Related Articles | Metrics

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Study on Aging Changes in Diameter of Root and Root Canal of Maxillary Central Incisors in Uygur Patients WU Long, Meng Fan-qi, DAI Yong-gang, ZHAO Jin 2017, 33(11): 1185-1188.  DOI: 10.13701/j.cnki.kqyxyj.2017.11.013 Abstract ( 225 )   PDF (1357KB) ( 197 )   Objective: To study the correlation between root and root canal diameter of the maxillary central incisors and age in the Uygur patients by cone-beam computed tomography (CBCT). Methods: CBCT images of 360 maxillary central incisors in Uygur patients were collected and divided into 6 groups at 10 year interval. Root and root canal diameters of maxillary central incisors at the 3/4 level from the cemenal-enamel junction to root apex were measured, and their correlation with gender and age were analyzed. Results: The mean root diameter (mm) in males and females were 6.14±0.348 and 5.89±0.396, respectively, the mean root canal diameter (mm) were 1.51±0.344 and 1.25±0.316, respectively, and the mean ratios between the root canal diameter and the root diameter for male and female were 0.25±0.059 and 0.21±0.057, respectively. There was significantly difference between genders (P＜0.05). There was negative correlation between the ratio of root canal diameter to root diameter and age (R= -0.659,P＜0.05). Conclusion: The root canal diameter of maxillary central incisors is correlated with age in Uygur patients, however, not correlated with age. References | Related Articles | Metrics

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Clinical and Radiographic Outcomes in Immature Permanent Teeth with Apical Periodontitis Treated with Pulp Revascularization Procedure LI Ling, MEI Li-qin, LI Jun, WEI Zhi-yuan 2017, 33(11): 1189-1192.  DOI: 10.13701/j.cnki.kqyxyj.2017.11.014 Abstract ( 366 )   PDF (1319KB) ( 472 )   Objective: To evaluate the clinical and radiographic effects of pulp revascularization procedure on immature teeth with apical periodontitis. Methods: In this study, clinical and radiographic data were collected every 3 months from 20 immature permanent teeth with apical periodontitis treated with a revascularization protocol for 18 months. Clinical and radiographic evidence of healing was evaluated, and radiographic changes in the radiographic root area and apical diameter were quantified. Results: After a follow-up period of 18 months, all teeth demonstrated radiographic evidence of complete periapical healing in the absence of clinical symptoms. Five teeth showed a positive response to pulp sensibility test after 18 months. The within-case percent change in radiographic root area was 28.13% after 3 months and had increased to 99.28% after 18 months. The within-case percent change in apical diameter after 3 month was 21.43% and had increased to 77.05% by 18 months, with 50% (10 of 20) showing complete apical closure after 18 months. Conclusion: In this study, pulp revascularization allowed the continued development of roots in immature permanent teeth with apical periodontitis. References | Related Articles | Metrics

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Efficacy of Different Techniques on Removal of Calcium Hydroxide in Curved Root Canals: A Cone-Beam Computed Tomographic Study BAI Yu-hao, HOU Xiao-mei, SU Zheng 2017, 33(11): 1193-1196.  DOI: 10.13701/j.cnki.kqyxyj.2017.11.015 Abstract ( 294 )   PDF (961KB) ( 465 )   Objective: To compare the efficacy of five techniques on removal of calcium hydroxide in curved canals. Methods: Fifty simulated resin canals with 45°curvature were prepared and randomly divided into 5 groups (n=10). Calcium hydroxide was injected into the root canals, and then the samples were scanned by cone beam CT to evaluate the volume of calcium hydroxide. Five techniques including needle irrigation, PIPS laser-activated irrigation, ultrasonic irrigation, sonic irrigation, and XP-endo finisher file were used to remove the calcium hydroxide. After cone beam CT scanning, the volume of the residual calcium hydroxide and the reduction rate of calcium hydroxide were calculated. The data were analyzed using Kruskal-Wallis H test at the significance level of P＜0.05. Results: The residual calcium hydroxide volume was 0mm3 (0mm3, 0.08mm3) in the PIPS laser-activated irrigation group, which was lower than those of other groups (P＜0.05). The reduction rate of calcium hydroxide was 100.00% (98.89%, 100.00%) in the PIPS laser-activated irrigation group, which was higher than those of other groups (P＜0.05). Reduction rate in the ultrasonic irrigation group and XP-endo finisher group were higher than that of sonic irrigation group (P＜0.05). Conclusions: None of five techniques could completely remove the calcium hydroxide from the curved root canals. Compared with other four techniques, PIPS laser-activated irrigation was significantly more effective in the removal of calcium hydroxide in the curved canals. References | Related Articles | Metrics

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Study on Removal of Root Canal Smear Layer by Qmix, MTAD and EDTA YIN Yan-jiao, GE Qiao-cheng, DING Xin-xin, WU Pei-ling 2017, 33(11): 1197-1200.  DOI: 10.13701/j.cnki.kqyxyj.2017.11.016 Abstract ( 370 )   PDF (1296KB) ( 414 )   Objective: To compare the removal of smear layer of root canal by Qmix, EDTA and MTAD using scanning electron microscope(SEM). Methods: Forty human mandibular premolar teeth with single canal were taken and decoronated to standardize the canal length to 14mm. Tooth were prepared by S3 rotary system to an root canal preparation of file size 3S, and then randomly divided into 4 groups according to the different final irrigations: 5ml saline for 1 min (Group A; negative control), 5ml 17% EDTA for 1 min (Group B), 5ml MTAD for 1min (Group C), and 5ml Qmix for 1 min (Group D). After the completion of root canal preparation, the teeth were split into two halves longitudinally. The smear layers in the root canal of apical 1/3 (Apical 1-2mm), root 1/3 (Apical 6-7mm), and root 1/3 (Apical 10-12mm) were observed with SEM, and the data were statistically analyzed. Results: The results of group B, C, D group were statistically significant different from that of group A (control group) (P<0.001). And group D was superior to group B and C, group C was better than group B (P<0.001). The coronal thirds of each group was better than the middle thirds of root, and the middle thirds of root was superior to the apical thirds of root (P<0.05). Conclusion: Qmix can effectively remove the root canal smear layer. References | Related Articles | Metrics

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Comparative Study of Four Kinds of Temporary Restorative Materials on Coronal Microleakage WANG Xue, WANG Bing-jie, GE Qiao-cheng, YIN Yan-jiao, MA Yu, DING Xin-xin, WU Pei-ling 2017, 33(11): 1201-1203.  DOI: 10.13701/j.cnki.kqyxyj.2017.11.017 Abstract ( 346 )   PDF (1080KB) ( 357 )   Objective: To observe the coronal microleakage of four kinds of temporary restorative materials after endodontic treatment under the stereomicroscope. Methods: Eighty freshly extracted teeth were collected and divided into four experimental groups. Four kinds of temporary restorative materials were used after endodontic treatment, which were glass ionomer cement (GIC), HY-BOND (HB) temporary cement, Cavition, and Clip F. After restored in water bath of 37℃ for 24 hours, the specimens were soaked in 1% Carbon ink for 7 days under room temperature. Then the specimens were cut buccolingually into two pieces with carborundum disc. The degree of microleakage was observed with stereomicroscope. Results: There were significant differences among four temporary materials (P<0.05). The degree of the coronal microleakage was: HY-BOND temporary cement>Cavition>GIC＞Clip-F (P＜0.05). Conclusion: Clip-F is an effective temporary restorative material. References | Related Articles | Metrics

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Effect and Functioanl Role of miR-146a in Oral Squamous Cell Carcinoma Tissues and Cell Lines WANG Li-ping, GUO Xue-qi, YAN Yong-yong, ZHA Jun, CHEN Wei-hong, WEI Yong-xiang, ZHU Xin-xin, GUAN Hong-bing, GE Lin-hu 2017, 33(11): 1204-1208.  DOI: 10.13701/j.cnki.kqyxyj.2017.11.018 Abstract ( 404 )   PDF (4105KB) ( 252 )   Objective: To explore the expression and its functional role of miR-146a in oral squamous cell carcinoma (OSCC) tissues. Methods: RT-PCR was performed to detect the expression level of miR-146a in OSCC tissues and OSCC cell lines. CCK8 assay, transwell assay and wound healing assay were performed to observer cell proliferation, invasion and migration abilities. Results: The expression of miR-146a was significantly higher in OSCC tissues compared to the adjacent tissues (P<0.05), and the expression of miR-146a in OSCC cell lines such as Scc9, Scc25, and Cal27 were substantially higher than the normal oral epithelial cells (HOK) (P<0.05). The proliferation, invasion and migration abilities were substantially enhanced in Scc9 and Cal27 cells transfected with miR-146a mimics (P<0.05). The invasion and migration abilities were substantially decreased in Scc9, Scc25, and Cal27 cells transfected with miR-146a inhibitor (P<0.05), but the proliferation abilities showed no statistically diffierents in Scc9, Scc25, and Cal27 cells transfected with miR-146a inhibitor. Conclusion: miR-146a shows a significant expression in OSCC tissues and cell lines, is correlated with proliferation, invasion and migration of OSCC. References | Related Articles | Metrics

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Expression of SOX2 in OSCC and Its Correlation with EMT XU Fei, WANG Shan, MENG Yan, SHI Lei, QIANG Dong-xia, ZHAO Er-yang 2017, 33(11): 1209-1212.  DOI: 10.13701/j.cnki.kqyxyj.2017.11.019 Abstract ( 202 )   PDF (1451KB) ( 202 )   Objective: To investigate the expression of SOX2 in OSCC and to evaluate its relationship with the expression of E-cad and Vim. Methods: The expressions of SOX2, E-cad, and Vim in 50 patients with oral squamous cell carcinoma and 13 specimens of oral pericancerous epithelium were detected by immunohistochemical staining method. Statistical analysis was performed with SPSS 19.0 software package. Results: The expression of SOX2 in oral epithelium was negative and the expression of SOX2 increased with the decrease of tissue type in OSCC (P<0.05). The expression of E-cad in oral epithelium was positive and the expression of E-cad decreased with the decrease of tissue type in OSCC (P<0.05). The expression of Vim in oral epithelium was negative and the expression of Vim increased with the decrease of tissue type in OSCC (P<0.05). In OSCC, the expressions of SOX2 and E-cad were negatively correlated (P<0.05), the expressions of SOX2 and Vim were positively correlated (P<0.05), and the expressions of E-cad and Vim were negatively correlated (P<0.05). Conclusion: Various expressions of SOX2 were found in different histological grade while the expression enhanced with the degrading of the differentiation degree, and there were negative correlations between SOX2 and epithelial mesenchymal transformation marker E-cad, and positive correlations between SOX2 and the marker Vim. References | Related Articles | Metrics

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Preparation and Cell Biology Imaging with Tryptophan Carbon Dots Tang Qiu-Ling, ZHAO Xiao-huan, YANG Ming-xi, PAN Jia-hui, LI Ge-ge, MENG Yang, WANG Liu-ran, YU Wei-xian 2017, 33(11): 1213-1217.  DOI: 10.13701/j.cnki.kqyxyj.2017.11.020 Abstract ( 235 )   PDF (1875KB) ( 347 )   Objective: To investigate the imaging characteristics of different cells that labeled by carbon dots (CDs) with multicolor fluorescence properties. Methods: Tryptophan CDs were synthesized by hydrothermal method and characterized by TEM, FT-IR, and fluorescence spectrometer. MTT method was used to detect CDs at different concentrations co-cultured with RAW264.7, MC3T3-E1, and L929 for 24h, which were observed under confocal microscope. The optimum imaging concentration of CDs was determined. Results: TEM showed that CDs were spherical particles with excellent dispersion and particle size of about 3.72nm. FTIR showed that molecular structure of CDs was consisted of carbon, oxygen, hydrogen, and nitrogen elements. Fluorescence spectra showed that at different excitation wavelengths, CDs could present different colors with excitation dependencies. MTT results showed that the cell activity of RAW264.7, MC3T3-E1, and L929 were up to 67%, 79% and 89% when the concentration of CDs was 400mg/L. The results of cell imaging showed that green and red fluorescence images were observed at 488 nm and 543 nm excitation wavelengths. The fluorescence of CDs was mainly detected in cell membrane and cytoplasm of RAW264.7 and L929. The entire MC3T3-E1 cell showed fluorescence, which indicated that the CDs might partially enter into its nucleus. Conclusion: Tryptophan CDs can be applied to cell imaging to facilitate observation and analysis of cell morphology and physiological activities. References | Related Articles | Metrics

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Three-dimensional Finite Element Analysis of Torsion Performance of Tongue Side Brackets BAI Rui, WEI Dai-lun, HE Han, Wu Xi, ZHOU Rong, HUANG Yue 2017, 33(11): 1218-1222.  DOI: 10.13701/j.cnki.kqyxyj.2017.11.021 Abstract ( 214 )   PDF (1543KB) ( 236 )   Objective: To explore the difference between two kinds of lingual brackets with different colpus when loaded torsion force with three dimensional finite element analysis. Method: Three dimensional finite element models were created which included IncognitoTM and Kurz7th brackets from A3 to B3, and assembled with ligature wires and wires of different types and materials. The data were analyzed and graphs of the models were drawn. Results: The load of IncognitoTM was 0 when assembled with 0.012 inch wires, and the other models’ deflection curve of two kinds of lingual brackets was similar. When assembled with the same wire, the max load value of Kurz7th was 10-12 times more than Incognito’s. When assembled with SS, TMA, and NiTi wire, which were the same size but different materials, their max load ratio of two kinds of brackets was 3.3, 1.3, and 1, respectively, and the load deflection curves were the same. When assembled with the wires of the same materials but different size, the load value of brackets increased with increasing size of wires. Conclusion: To correct torsion teeth, IncognitoTM has a limited effect but Kurz7th seems to be a good choice, and the length, width, and depth of two kinds of colpusall have an influence to the results. References | Related Articles | Metrics

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Clinical Characteristics and Surgical Treatment of Cervical Necrotizing Fasciitis Associated with Descending Necrotizing Mediastinitis YANG Xu, XU Tian-shu, ZHENG Liang 2017, 33(11): 1223-1225.  DOI: 10.13701/j.cnki.kqyxyj.2017.11.022 Abstract ( 246 )   PDF (1012KB) ( 210 )   Objective: To retrospectively review 5 cases of cervical necrotizing fasciitis associated with descending necrotizing mediastinitis (CNF/DNF), and to investigate the clinical characteristics and surgical treatment of this complex infection. Methods: Five patients, who underwent extensive cervical drainage, were identified from December 2012 to December 2016. Besides, two of them underwent the two-stage mediastinoscope drainage of mediastinum. Results: One patient was successfully treated after extensive cervical drainage, while other two patients who did not get efficient drainage of mediastinum were died of multiorgan failure related to septic shock. The rest patients who had undergone the two-stage mediastinoscope drainage of mediastinum were successfully treated. The mortality rate was 40%. Conclusion: High mortality is found in this complex infection. As it progresses quickly, prompt diagnosis and surgical treatment are essential for reducing mortality in CNF/DNM. Extensive cervical drainage and transthoracic drainage should be performed simultaneously without delay. Cervical drainage and mediastinoscope drainage is worthy of promotion. References | Related Articles | Metrics

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Variation of Root Canals in Maxillary Second Molar: A Case Report ZHANG Mei, NI Can, REN Shuang-shuang, LI Wen, YU Yi-jun, MIAO Lei-ying 2017, 33(11): 1226-1227.  DOI: 10.13701/j.cnki.kqyxyj.2017.11.023 Abstract ( 400 )   PDF (1096KB) ( 384 )   Root canal system is really complicated since it has a lot of variation, especially in the maxillary second molar. The root canals can have different number and configuration. The present article reports a rare case about successful treatment of a maxillary second molar with five canals. References | Related Articles | Metrics

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Application of Autologous Tooth as Bone Graft Material in Upper Anterior Teeth Implant Restoration: A Case Report JIN Xia-ying, ZHONG Wei-jian, MA Guo-wu 2017, 33(11): 1228-1229.  DOI: 10.13701/j.cnki.kqyxyj.2017.11.024 Abstract ( 312 )   PDF (944KB) ( 233 )   Objective: To investigate the clinical effects of autologous tooth as bone graft material in upper anterior teeth immediate implant placement. Methods: After anterior teeth were extracted, two implants were placed into the fresh extraction socket. Guided bone regeneration (GBR) was performed and mixture of platelet rich-fibrin (PRF) and autologous tooth particles were placed in the bone defect around implant. Results: The radiograph demonstrated that the space around implant was filled with bone-like tissue and the soft tissue was sufficient. Conclusion: Autologous tooth particle can be used as bone grafting material in reconstruction of bone defect around implant. References | Related Articles | Metrics

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Effect and Progress of Autophagy Induced by Nanoparticles on Inhibition of Tumor Growth and Treatment WANG Yi-bo, SUN Bing, WANG Dan-dan, TANG Qi, SUN Hong-chen 2017, 33(11): 1230-1233.  DOI: 10.13701/j.cnki.kqyxyj.2017.11.025 Abstract ( 347 )   PDF (792KB) ( 344 )   Cell autophagy is an important physiological response of cell self-regulation. It is closely related to nanoparticles. In recent years, nanoparticle and autophagy have become a hotspot in biomedical research. The results show that the effective induction of autophagy by nanoparticles in the specific cells can accelerate the death of diseased cells, which has important significance for the treatment of tumors and nervous system diseases. In this paper, the progress of autophagy and apoptosis and the mechanism of autophagy and apoptosis were introduced. The autophagy induced by different nanoparticles was discussed. The application of nanomaterials in autophagy was also discussed. It is our duty to focus on the study of mechanism of nanoparticle and cell autophagy and the controllability of nanoparticles so as to better apply to life science and help to cure diseases. References | Related Articles | Metrics

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Relationship between Interleukin-34 and Periodontitis and Peri-implantitis WU Li-li,Shi Qiu-tao,GU Zhi-yuan 2017, 33(11): 1234-1236.  DOI: 10.13701/j.cnki.kqyxyj.2017.11.026 Abstract ( 247 )   PDF (771KB) ( 206 )   Periodontitis and peri-implantitis influence the periodontal tissues, and result in the supporting bone loss and even tooth and implant loss. Interleukin-34 is a tolerogenic cytokine sharing the same receptor--M-CSFR with M-CSF, and is a stimulator of osteoclasts differentiation that exerts synergistic effects with RANKL and might perform an important function in the occurrence and development of periodontitis and peri-implantitis. In this paper, the mechanism of bone resorption and immunoregulatory function of interleukin-34 is summarized, which is greatly significant for understanding the pathogenic mechanism and clinical treatment of periodontitis and peri-implantitis. References | Related Articles | Metrics