Effect of N-acetyl Cysteine on Lipopolysaccharide Induced Expressions of Genes and Proteins in MC3T3-E1 Osteoblasts

doi:10.13701/j.cnki.kqyxyj.2017.11.005

Abstract

Abstract: Objective: To evaluate the effect of N-acetyl cysteine on Lipopolysaccharide induced expressions of genes and proteins in MC3T3-E1 osteoblasts. Methods: The osteoblasts were isolated from the calvarias of mice and cultured in a-MEM. Cells were stimulated with various concentrations of NAC or LPS and then the proliferation of the cells was observed at different time points (6h, 24h, 48h, and 72h) by CCK-8. The expression of ALP, BGP, NF-KB, and IL-6 were determined by qPCR and ELISA analyses. Results: The proliferation of the cells increased with the increasing concentrations of NAC. When the concentration of NAC was 1mmol/L, the proliferation of the cells reached the maximum. A higher concentration of NAC had toxic effects. The down-regulated gene and protein expressions of ALP and BGP induced by 10μg/ml LPS were promoted significantly after treated with 1mmol/L NAC. The up-regulated gene and protein expressions of IL-6 and NF-kB induced by 10μg/ml LPS were decreased significantly after treated with 1mmol/L NAC. Conclusion: Expressions of ALP and BGP induced by LPS were promoted significantly after treated with NAC. NAC could decrease the expression of IL-6 induced by LPS through the NF-kB signaling.

Key words: N-acetyl cysteine, Lipopolysaccharide, MC3T3-E1 osteoblast, NF-kB, IL-6

CLC Number:

R329.2

ZHANG Hui, LI Wang-yang, HUANG Ying, CAI Yue, GUO Ling. Effect of N-acetyl Cysteine on Lipopolysaccharide Induced Expressions of Genes and Proteins in MC3T3-E1 Osteoblasts[J]. Journal of Oral Science Research, 2017, 33(11): 1151-1155.

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