Abstract: Objective: To screen and identify miRNA which regulate gene expression of BSP and to analyze miRNA to regulate BSP gene expression during the osteogenic differentiation of hPDLCs depressed by LPS from P.g. Methods: Bioinformatics prediction and dual luciferase reporter gene analysis system were used to screen and verify the miR-129 that could be targeted to regulate BSP gene. miR-129 overexpression and expression inhibition vector plasmids were obtained to transfect hPDLCs. After treated each group of cells with 10mg/L P.g. LPS for 8 hours, the expression level of BSP was detected by qRT-PCR and western blotting. Results: Treatments with 10mg/L of P.g. LPS for 8 hours up-regulated the expression of miR-129, whereas BSP mRNA expression was induced. The expression levels of BSP in the experimental groups were lower than those in the mimic control group when miR-129 expression increased (P＜0.05). The protein expression levels of BSP in the experimental groups were higher than those in the inhibitor control group when miR-129 expression decreased(P＜0.05). Conclusion: In the process of P.g. LPS affecting osteogenic activity of hPDLCs, miR-129 may reduce its osteogenic activity by inhibiting BSP.

Key words: microRNA, porphyromonas gingivalis, lipopolysaccharide, bone sialoprotein, bone destruction