Effects of MicroRNA-218-5p Targeting ABCG2 Gene on Proliferation, Migration, and Invasion of Tca-8113 Cells.

doi:10.13701/j.cnki.kqyxyj.2019.10.018

Abstract

Abstract: Objective: To investigate the effects of miR-218-5p targeting ABCG2 gene on the proliferation, migration, and invasion of Tca-8113 cells and its mechanism. Methods: qRT-PCR and western blot were used to detect the expression of miR-218-5p and ABCG2 in Tca-8113 and NOK cells. The targeting relationship between miR-218-5p and ABCG2 was detected by dual luciferase reporter gene experiment. miR-218-5p mimic, ABCG2 siRNA and miR-218-5p mimic+pcDNA-ABCG2 were transfected into Tca-8113 cells by liposome method. The proliferation, migration, and invasion of Tca-8113 cells were detected by MTT and Transwell tests. Results: Compared with NOK cells, miR-218-5p was significantly down-regulated in Tca-8113 cells, and ABCG2 was significantly expressed (P<0.05). Over-expression of miR-218-5p and inhibition of ABCG2 inhibited proliferation and migration of Tca-8113 cells (P<0.05). There is a targeted binding relationship between miR-218-5p and ABCG2. Over-expression of ABCG2 reverses the inhibitory effect of up-regulated miR-218-5p on proliferation, migration, and invasion of Tca-8113 cells. Conclusion: Up-regulating the expression of miR-218-5p can target ABCG2 to inhibit the proliferation, migration, and invasion of Tca-8113 cells, which may provide a new target for the treatment of OSCC.

Key words: oral squamous cell carcinoma, microRNA-218-5p, ATP-binding cassette sub-family G member 2, proliferation, migration, invasion

WEI Wei, TANG Yi, DAI Jing, CHEN Hujie. Effects of MicroRNA-218-5p Targeting ABCG2 Gene on Proliferation, Migration, and Invasion of Tca-8113 Cells.[J]. Journal of Oral Science Research, 2019, 35(10): 984-988.

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