Effects of Canonical Wnt Signaling Pathway on Proliferation, Migration, and Odontogenic Differentiation of hDPSCs

doi:10.13701/j.cnki.kqyxyj.2020.09.014

Abstract

Abstract: Objective:To investigate the effects of canonical Wnt/β-catenin signaling pathway on proliferation, migration, odontogenic differentiation, and mineralization of human dental pulp stem cells (hDPSCs). Methods: The optimal concentration of DKK1 was chosen to inhibit Wnt signaling pathway and treated hDPSCs. The proliferation and migration of hDPSCs were determined by MTT and scratching assay. The effects of DKK1 on odontogenic differentiation of hDPSCs were detected by Western Blot and ALP staining. Alizarin red was carried out to explore the effects of DKK1 on the mineralization ability of hDPSCs at the early stage of mineralization. Results: MTT test showed that the A of hDPSCs in the DKK1 group was higher than that in the control group on the 5th day(P<0.05). Scratching assay showed that the cells migrated from the scratch edge to the middle, and after 24h the cell migration distance of the DKK1 group was significantly larger than that of control group. Alizarin red staining showed that multiple mineralized nodules at various sizes were observed in the DKK1 group. Western blot results showed that DKK1 up-regulated the protein expression of DMP-1. Conclusion: Inhibiting canonical Wnt signaling pathway promoted the proliferation, migration, odontogenic differentiation, and mineralization of hDPSCs.

Key words: Dikkopf-1, human dental pulp stem cells, proliferation, migration, odontogenic differentiation

GUAN Mengying, HE Lina, PAN Shuang, LI Yanping, LIU Huimei, NIU Yumei. Effects of Canonical Wnt Signaling Pathway on Proliferation, Migration, and Odontogenic Differentiation of hDPSCs[J]. Journal of Oral Science Research, 2020, 36(9): 861-865.

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