Naringin in Nanometer Hydroxyapatite Collagen Scaffold Enhanced the Proliferation and Osteoblastic Differentiation of Human Dental Pulp Stem Cells in Vivo

doi:10.13701/j.cnki.kqyxyj.2019.08.006

Abstract

Abstract: Objective: To evaluate the effects of naringin-hydroxyapatite collagen scaffold (Nar-HA/COL) on proliferation and osteoblastic differentiation of human dental pulp stem cells (hDPSCs). Methods: HDPSCs were isolated and cultured on Nar-HA/COL scaffolds. CCK-8 was used to detect the proliferation ability. The osteoblastic differentiation potentials were assessed by alkaline phosphatase assay kit, Alizarin red S histochemical staining, and western Blot. Results: The proliferation ability, ALP activity, mineralization potential, RUNX-2, and BMP-2 proteins expression of hDPSCs were enhanced by 0.5% Nar-HA/COL scaffolds. Conclusion: The results showed Nar-HA/COL scaffolds could enhance the proliferation and osteoblastic differentiation abilities of hDPSCs.

Key words: naringin, hydroxyapatite collagen scaffold, human dental pulp stem cells, osteoblastic differentiation

HOU Tingting, PAN Shuan, LI Yanping, HE Lina, XIAO Wanlu, NIU Yumei. Naringin in Nanometer Hydroxyapatite Collagen Scaffold Enhanced the Proliferation and Osteoblastic Differentiation of Human Dental Pulp Stem Cells in Vivo[J]. Journal of Oral Science Research, 2019, 35(8): 744-747.

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