Functional Role of Rho Kinase Inhibitor Y-27632 on Proliferation of Human Dental Pulp Stem Cells.

doi:10.13701/j.cnki.kqyxyj.2016.03.016

Abstract

Abstract: Objective: To study the influence of Rho kinase inhibitor Y-27632 on the proliferation of human dental pulp stem cells. Methods: Human dental pulp stem cells (hDPSCs) were seeded in plates，then divided randomly into two groups: cultured with or without Y-27632. Cellular proliferation was determined by DAPI staining and MTT assay. Cell cycle was analyzed by flow cytometry (FCM). Results: After 24, 48, 72 and 96h of cultivation, the optical density (OD) in the Y-27632 treated group was significantly higher than that in the control group. Moreover, the peak time during proliferation was earlier in the treat group. After 48h cultured, compared with the control group, the DAPI staining showed that the proportion of total cells was significantly increased in the Y-27632 treated group. The FCM results indicated that (66.8±6.84)% of the hDPSCs were in G0/G1 phase (25.17±0.62)% in S phase. After Y-27632 treatment, (58.59±1.76)% of the hDPSCs were in G0/G1 phase (31.34±1.16)% in S phase. The cell proliferation during S phase was significantly higher after stimulation by Y-27632 than that in the control group (P<0.05). Conclusion: Inactivation of Rho kinase in the hDPSCs by inhibitor Y-27632 led more hDPSCs to entering into the DNA synthesis stage and promoted the division and proliferation of hDPSCs.

Key words: Human dental pulp stem cells , Rho kinase , Y-27632 , Cell proliferation

CLC Number:

R780.1

YU Tian-yao, PAN Shuang, HE Li-na, LI Yan-ping, ZHANG Lin, NIU Yu-mei. Functional Role of Rho Kinase Inhibitor Y-27632 on Proliferation of Human Dental Pulp Stem Cells.[J]. Journal of Oral Science Research, 2016, 32(3): 278-281.

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