Effects of Taccaloniolde on LPS - stimulated Proliferation of Periodontal Ligament Fibroblasts

doi:10.13701/j.cnki.kqyxyj.2018.06.019

Abstract

Abstract: Objective: To research the effect and mechanism of taccaloniolde on LPS-stimulated proliferation of human periodontal ligament fibroblasts. Methods: Human periodontal ligament fibroblasts were cultured in LPS-containing medium with or without different concentrations of taccaloniolde. At the same time, the blank control group without taccaloniolde was established. After 24, 48, and 72 hours, the cell proliferation was detected by CCK-8 assay and the cell apoptosis was detected by flow cytometry. Results: After 24, 48, and 72 h of drug addition, the relative cell viability in the different doses of taccaloniolde was significantly lower than that stimulated with the control and LPS. Compared with the control group, taccaloniolde could promote apoptosis significantly. Conclusion: Taccaloniolde can significantly inhibited cell proliferation of human periodontal ligament fibroblasts increased by LPS-stimulated, and its possible mechanism is due to promote apoptosis.

Key words: Taccaloniolde, Fibroblasts, Cell proliferation, Apoptosis

ZHOU Qian-wen, ZENG Hao, ZHANG Jia-xin, LIU Gong-qi, WANG Sha, WANG Lie. Effects of Taccaloniolde on LPS - stimulated Proliferation of Periodontal Ligament Fibroblasts[J]. Journal of Oral Science Research, 2018, 34(6): 653-656.

References

[1] Tsutsumi T, Takeshi T, Nakashima K, et al. Involvement of adhesion molecule in in vitro plaque-like formation of macrophag es stimulated with Agg reg atibacter actinomy cetemcomitans lipopoly saccharide [J]. J Periodontal Res, 2010, 45(4):550-556
[2] Johnson GK, Guthmiller JM, Joly S, et al. Interleukin-1 and interleukin-8 in nicotine and lipopoly saccharidex posed going ival keratinocy te cultures [J]. J Periodontal Res, 2010, 45(4):583-588
[3] 付昌盛, 刘荣森, 罗芸,等.内毒素对人牙周膜成纤维细胞增殖及IL-8分泌的影响[J].中华老年口腔医学杂志,2017,15(2):82-86
[4] Liu HY, Ni W, Hao XJ, et al. Steroidal saponins from Tacca plantaginea [J]. J Asian Nat Prod Res, 2006, 8(4):293-298
[5] 张鹏, 张旭. 以细胞骨架为靶点抗癌药物的研究进展[J].解放军医学院学报, 2013,34(6):661-663
[6] Risinger AL, Jackson EM, Polin LA, et al. The taccalonolides are a novel class of microtubule stabilizers that circumvent clinically relevant taxane resistance mechanisms [J]. Cancer Res, 2009, 69(2 Supplement)
[7] Tinley TL, Randall-Hlubek DA, Leal RM, et al. Taccalonolides E and A: Plant-derived steroids with microtubule-stabilizing activity [J]. Cancer Res, 2003, 63(12):3211-3220
[8] Soory M. Bacterial steroidogenesis by periodontal pathogens and the effect of bacterial enzymes on steroid conversions by human gingival fibroblasts in culture [J]. J Periodontal Res, 1995, 30(2):124-131
[9] Soory M. Targets for steroid hormone mediated actions of periodontal pathogens, cytokines and therapeutic agents: some implications on tissue turnover in the periodontium [J]. Curr Drug Targets, 2000, 1(4):309-325.
[10] Samietz S, Holtfreter B, Friedrich N, et al. Prospective association of sex steroid concentrations with periodontal progression and incident tooth loss [J]. J Clin Periodontol, 2015, 43(1):10-18
[11] El-Aw ady AR, Lapp CA, Gamal AY, et al. Human periodontal ligament fibroblast responses to compression in chronic periodontitis [J]. J Clin Periodontol, 2013, 40(7):661-671
[12] Wang Y, Yu Y, Li GB, et al. Mechanism of microtubule stabilization by taccalonolide AJ [J]. Nat Commun, 2017, 8:15787
[13] Al-Khalili L, Krook A, Chibalin AV.Phosphorylation of the Na⁺,K⁺-ATPase in skeletal muscle: potential mechanism for changes in pump cell-surface abundance and activity [J]. Ann N Y Acad Sci,2003,986:449-452
[14] Seubbuk S, Sritanaudomchai H, Kasetsuwan J, et al. High glucose promotes the osteogenic differentiation capability of human periodontal ligament fibroblasts [J]. Mol Med Rep, 2017, 15(5):2788-2794
[15] Zhang L, Ding Y, Rao GZ, et al. Effects of IL-10 and glucose on expression of OPG and RANKL in humanperiodontal ligament fibroblasts [J]. Braz J Med Biol Res, 2016, 49(4):e4324
[16] 张江琳,王浩宇,李丹,等.复方黄芩片对人牙周膜细胞及牙周炎牙槽骨OPG/RANKL表达的影响[J].口腔医学研究,2015,31(11):1064-1068