Plumbagin Induces Tongue Squamous Cell Carcinoma Cells Apoptosis via ROS-mediated JNK Signaling Pathway

doi:10.13701/j.cnki.kqyxyj.2020.10.008

Abstract

Abstract: Objective: To explore the underlying molecular mechanisms of plumbagin-induced apoptosis of tongue squamous cell carcinoma cells. Methods: The effect of plumbagin on cell proliferation was determined by CCK-8 assay. Cell apoptosis and the expression of cellular ROS generation were measured by flow cytometry. Protein levels of Bcl-2, Bax, and p-JNK were evaluated by Western blot. Results: CCK-8 assay showed that plumbagin significantly inhibited cell proliferation in a dose-dependent manner(P<0.001). The results of flow cytometer suggested that plumbagin markedly increased cell apoptosis and ROS generation. Western blot showed that the expression of Bax increased, while the expression of Bcl-2 decreased after plumbagin treatment. In a time-dependent manner, plumbagin up-regulated the expressions of p-JNK (P<0.05,P<0.01,P<0.001). However, the ROS inhibitor NAC significantly reversed the expression level of p-JNK and cell apoptosis(P<0.001,P<0.001). Conclusion: Plumbagin had excellent anti-proliferation effects on tongue squamous cell carcinoma cells. In addition, plumbagin induced cell apoptosis through ROS-mediated JNK pathway.

Key words: plumbagin, tongue squamous cell carcinoma, cell apoptosis, reactive oxygen species

ZHOU Qun, QIU Jiaxuan. Plumbagin Induces Tongue Squamous Cell Carcinoma Cells Apoptosis via ROS-mediated JNK Signaling Pathway[J]. Journal of Oral Science Research, 2020, 36(10): 921-924.

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