Ascorbic Acid Carbon Dots Kill Tongue Squamous Cell Carcinoma Cell by Promoting Autophagy

doi:10.13701/j.cnki.kqyxyj.2018.01.009

Abstract

Abstract: Objective: To study the lethal impact and mechanism of ascorbic acid carbon dots (AACDs) on tongue squamous cell carcinoma cell line Cal27. Methods: The blank control group was untreated Cal27, while the groups treated with AACDs, 3-methyladenine, and both of them were the experimental groups. MTT assay was used to detect the cell proliferation. Acridine orange staining was used to evaluate the cell autophagy. Flow cytometry was performed to examine cell apoptosis. Results: AACDs suppressed the proliferation of Cal27 on a dose-dependent manner. It also induced the autophagy of Cal27, especially in the 100 μg/ml AACDs group. When 3-methyladenine was added to induce the autophagy level, cell proliferation level was improved with the decrease of apoptotic rate. Conclusion: AACDs kill tongue squamous cell carcinoma cells through promoting autophagy.

Key words: Ascorbic acid carbon dots, Tongue squamous cell carcinoma, Autophagy, Nanoparticle, 3-methyladenine

CLC Number:

R739.8

LIU Yu-lan, MENG Lin, TANG Qi, BU Wen-huan, HUANG Guo-shuai, DAI Yi-bin, LIU Qi-lin, SUN Hong-chen. Ascorbic Acid Carbon Dots Kill Tongue Squamous Cell Carcinoma Cell by Promoting Autophagy[J]. Journal of Oral Science Research, 2018, 34(1): 35-38.

References

[1] Xie Y, Murray-Stewart T, Wang Y, et al. Self-immolative nanoparticles for simultaneous delivery of microRNA and targeting of polyamine metabolism in combination cancer therapy [J]. J Control Release,2017,246(1)∶110-119
[2] Conde J,Oliva N,Zhang Y,Artzi N. Local triple-combination therapy results in tumour regression and prevents recurrence in a colon cancer model [J]. Nat Mater,2016,15(11)∶1128-1138
[3] Elgogary A,Xu Q,Poore B,Alt J,et al. Combination therapy with BPTES nanoparticles and metformin targets the metabolic heterogeneity of pancreatic cancer [J]. Proc Natl Acad Sci USA,2016,113(36)∶E5328-E5336
[4] Gilam A,Conde J,Weissglas-Volkov D,et al. Local microRNA delivery targets Palladin and prevents metastatic breast cancer [J]. Nat Commun,2016,7∶12868
[5] Duran N,Simoes MB,de Moraes ACM,et al. Nanobiotechnology of carbon dots: a review [J]. J Biomed Nanotechnol, 2016, 12(7)∶1323-1347
[6] Kim AD,Kang KA,Kim HS,et al. A ginseng metabolite,compound K,induces autophagy and apoptosis via generation of reactive oxygen species and activation of JNK in human colon cancer cells [J]. Cell Death Dis,2013,4∶e750
[7] Maes H,Rubio N,Garg AD,et al. Autophagy:shaping the tumor microenvironment and therapeutic response [J]. TRENDS MOL MED.2013,19(7)∶428-446
[8] Lee YH,Cheng FY,Chiu HW,et al. Cytotoxicity,oxidative stress,apoptosis and the autophagic effects of silver nanoparticles in mouse embryonic fibroblasts [J]. Biomaterials,2014,35(16)∶4706-4715
[9] Su M,Mei Y,Sinha S. Role of the crosstalk between autophagy and apoptosis in cancer [J]. J Oncol,2013,2013∶1-14
[10] Macintosh RL, Ryan KM. Autophagy in tumour cell death [J]. SEMIN CANCER BIOL,2013,23(5)∶344-351
[11] Xia LL,Wang Y,Chen Y,et al. Cuprous oxide nanoparticles inhibit the growth of cervical carcinoma by inducing autophagy [J]. Oncotarget,2017,8(37)∶61083- 61092
[12] Yu YB,Duan JC,Yu Y,et al. Autophagy and autophagy dysfunction contribute to apoptosis in HepG2 cells exposed to nanosilica [J]. Toxicol Res,2016,5∶871-882
[13] Lu HY,Chang YJ,Fan NC,et al. Synergism through combination of chemotherapy and oxidative stress-induced autophagy in A549 lung cancer cells using redox- responsive nanohybrids: a new strategy for cancer therapy [J]. Biomaterials,2015,42∶30-41
[14] Shi M,Cheng L,Zhang ZB,et al. Ferroferric oxide nanoparticles induce prosurvival autophagy in human blood cells by modulating the Beclin 1/Bcl-2/VPS34 complex [J]. INT J NANOMED,2015,10∶207-216
[15] 章思莹,潘淑婷,帅芳源,等.PI3K/AKT/mTOR/p70S6K通路在人舌鳞癌细胞耐药中的作用[J].口腔医学研究,2017,33(9)∶938-942