Lentivirus Mediated Down-regulation of Med19 Expression Enhances Sensitivity of Tongue Squamous Cell Carcinoma to Cisplatin

doi:10.13701/j.cnki.kqyxyj.2019.06.012

Abstract

Abstract: Objective: To investigate the effect of down-regulation of lentivirus-mediated mediator 19 (Med19) expression on the sensitivity of tongue squamous cell carcinoma cells to cisplatin. Methods: Med19 shRNA was transfected into tongue squamous cell carcinoma cells. The interference effects were measured by qRT-PCR and Western blot. Cisplatin treated tongue squamous cell carcinoma with Med19 downregulation. MTT and flow cytometry were used to detect the cell proliferation and apoptosis. The levels of C-Caspase-3, C-Caspase-9, and Bax were detected by Western blotting, and the levels of Cyt-c in cytoplasm and mitochondria were detected by Western blotting. Results: The expression level of Med19 in tongue squamous cell carcinoma increased. Transfection of Med19 shRNA could reduce the expression level of Med19 in tongue squamous cell carcinoma. The proliferation of tongue squamous cell carcinoma treated with Med19 or cisplatin decreased, and the rate of apoptosis increased, the level of C-Caspase-3, C-Caspase-9 and Bax protein increased in cells. The expression level of Cyt-c protein in the cytoplasm increased, and the expression level of Cyt-c protein in mitochondria decreased. The effect of down-regulation of Med19 combined with cisplatin on the proliferation and apoptosis of tongue squamous cell carcinoma cells and the release of Cyt-c from C-Caspase-3, C-Caspase-9, Bax and mitochondria were greater. Conclusion: Down regulation of Med19 can enhance the sensitivity of tongue squamous cell carcinoma to cisplatin.

Key words: Med19, Tongue squamous cell carcinoma, Cisplatin, Apoptosis

WANG Rui, LI Li-heng, XU Ai-wen, AN Feng. Lentivirus Mediated Down-regulation of Med19 Expression Enhances Sensitivity of Tongue Squamous Cell Carcinoma to Cisplatin[J]. Journal of Oral Science Research, 2019, 35(6): 559-562.

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