TH287 Inhibits Proliferation and Metastasis of CAL27

doi:10.13701/j.cnki.kqyxyj.2019.07.020

Abstract

Abstract: Objective: To test the inhibition of TH287 on the proliferation and metastasis of CAL27. Methods: MTT assay was used to test the inhibition of TH287 on the proliferation of CAL27 and ascertain the appropriate concentration. The promotion of TH287 on the apoptosis of CAL27 was tested by the flow cytometry. The inhibition of TH287 on the MTH1 expression was examined by the western blot. The inhibition of TH287 on the metastasis of CAL27 was examined by the scratch test and Transwell (migration). Results: TH287 could inhibit the proliferation of CAL27 effectively and the appropriate concentration was 100 μM. TH287 could also promote the apoptosis of CAL27 and inhibit the MTH1 expression. In addition, the metastasis of CAL27 was also inhibited by the TH287. Conclusion: TH287 could inhibit the proliferation and promote the apoptosis of CAL27. Meanwhile, the metastasis of CAL27 was also inhibited by TH287. The inhibition of TH287 on the proliferation and metastasis of CAL27 may be caused by the suppression of MTH1 expression. Therefore, TH287 has great potential to be a new drug in targeted therapy.

Key words: CAL27 , TH287, proliferation, apoptosis, metastasis

SHAN Qiusheng, LI Guolin, XU Jiaqi. TH287 Inhibits Proliferation and Metastasis of CAL27[J]. Journal of Oral Science Research, 2019, 35(7): 704-707.

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