Functions of Platelet-rich Fibrin on Proliferation and Osteogenic Differentiation of Dental Pulp Stem Cells

doi:10.13701/j.cnki.kqyxyj.2020.10.019

Abstract

Abstract: Objective: To investigate the effects of platelet-rich fibrin on the proliferation and osteogenic differentiation of human dental pulp stem cells. Methods: Human dental pulp stem cells were treated with platelet-rich fibrin of different dilution concentrations, and the proliferative activity of human dental pulp stem cells was detected by MTT assay. Flow cytometry was used to detect the cell cycle. The expression level of bone differentiation markers ALP, COL-I, RUNX2, and OCN were measured by western blot. Results: When the concentration of platelet-rich fibrin reached more than 50%, the proliferation activity of human pulp stem cells was significantly enhanced, and more cells were induced to S phase. The expression of osteogenic differentiation markers ALP, COL-I, and RUNX2 were significantly up-regulated. When the concentration of platelet-rich fibrin reached 80%, the expression of OCN was also significantly increased. Conclusion: Platelet-rich fibrin can promote the proliferation and osteogenic differentiation of human dental pulp stem cells.

Key words: platelet rich fibrin, human dental pulp stem cells, proliferation, osteogenic differentiation

YAN Na, HUANG Tao, ZHANG Zhongyue, ZHANG Shuhua, WANG Lihua, HAN Guoliang. Functions of Platelet-rich Fibrin on Proliferation and Osteogenic Differentiation of Dental Pulp Stem Cells[J]. Journal of Oral Science Research, 2020, 36(10): 973-977.

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