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Advancements of Clinical and Basic Research in Malignant Hyperthermia. TANG Zhangui, CHEN Juan 2020, 36(10): 893-897.  DOI: 10.13701/j.cnki.kqyxyj.2020.10.001 Abstract ( 335 )   PDF (974KB) ( 360 )   Malignant hyperthermia (MH) is a rare genetic disease which characterized by a sharp rise in body temperature and a series of pathological changes caused by severe contraction of skeletal muscle when exposed to volatile anesthetics or depolarizing muscle relaxants. The patient is difficult to diagnose before the onset of the disease. The development MH is very rapid, and if not effectively treated, it is very easy to appear life-threatening. With the wide application of various anesthetics in the clinic, the probability to encounter MH is increasing gradually. However, the treatment methods are certain particular. Misdiagnosis and mistreatment may lead to the death of patients. Therefore, this paper reviews the clinical and basic research progress of MH. References | Related Articles | Metrics

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Status of Surgical Treatment to Cystic Lesions of Jaw DUAN Congcong, HAN Bing 2020, 36(10): 898-901.  DOI: 10.13701/j.cnki.kqyxyj.2020.10.002 Abstract ( 373 )   PDF (754KB) ( 395 )   The cystic lesion is one of the common diseases of the jaw, including the jaw cyst and the cystic tumor. The main surgical procedures are curettage, decompression, Dredging method, and resection. The choice of clinical specific surgery needs to be balanced according to the size and nature of the lesion, patient compliance, subjective will, and doctor's technology and conditions, in order to restore the facial morphology and function as much as possible. References | Related Articles | Metrics

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Clinical Features and Timing Selection for Extraction of Supernumarary Teeth in Maxillary Anterior Region SHA Zhenyu, WANG Liao 2020, 36(10): 902-904.  DOI: 10.13701/j.cnki.kqyxyj.2020.10.003 Abstract ( 458 )   PDF (727KB) ( 868 )   The definition of supernumarary teeth is that teeth come from dental sac in excess of the normal number. Mesiodens are the most common supernumerary teeth, occurring in 0.3%-0.8% in deciduous dentition and 1.5%-3.5% in permanent dentition. Supernumerary teeth always cause complications such as dental impaction, overcrowding, root dilacerations, or the formation of follicular cysts. The aim of this paper is to review the prevalence, etiology, diagnosis, treatment, and complications of Mesiodens. References | Related Articles | Metrics

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Research Progress on Functional Preservation of Submandibular Gland HUANG Qing, RUAN Min 2020, 36(10): 905-907.  DOI: 10.13701/j.cnki.kqyxyj.2020.10.004 Abstract ( 215 )   PDF (736KB) ( 323 )   The submandibular gland is one of three major salivary glands in the oral and maxillofacial region. It plays an important role in digestion, chewing, caries prevention, and other physiological functions. In recent years, more and more researches on the submandibular gland have been carried out at home and abroad. Preserving the function of submandibular gland has attracted the attention of scholars. The salivary secretion function of submandibular gland was kept by preserving the gland, which significantly improved the quality of life of the patients after operation. This article briefly reviews the significance and research progress of preservation of gland function from submandibular gland. References | Related Articles | Metrics

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Modulation of Osteoimmune Microenvironment by Adding Bioactive Elements to Implants Surface FU Wenqi, LIU Huiying 2020, 36(10): 908-911.  DOI: 10.13701/j.cnki.kqyxyj.2020.10.005 Abstract ( 297 )   PDF (769KB) ( 236 )   Osteoimmune microenvironment has profound influence on initial osseointegration, which is closely related to implants surface characteristics. The high diversity and plasticity of immune cells make it possible to manipulate the immune cells response and cytokines expression, thus modulating the osteoimmune microenvironment. Modified by the bioactive elements, such as Zn, Sr, Si, the implants surface can stimulate the osteogenic differentiation of bone marrow mesenchymal stem cells and increase the expression of anti-inflammatory cytokines in immune cells. Based on these complex interactions, this review discusses the strategies by adding bioactive elements to implants surface and highlights the possible underlying mechanisms of osteoimmunomodulation in order to guide the development of advanced implants. References | Related Articles | Metrics

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Application of Anterior Auricular Extension Incision in Resection of Accessory Parotid Gland Tumors HONG Xiaowei, SUN Guowen, WANG Zhiyong, ZHU Feng, LI Wei 2020, 36(10): 912-914.  DOI: 10.13701/j.cnki.kqyxyj.2020.10.006 Abstract ( 208 )   PDF (2427KB) ( 240 )   Objective: To evaluate the clinical effect of anterior auricular extension incision in resection of accessory parotid gland tumors. Methods: Eight cases diagnosed as accessory parotid gland tumors underwent tumor resection surgery using the anterior auricular extension incision. Clinical data such as operation time, postoperative complications, and patients' satisfaction with incision appearance were recorded and analyzed. Results: Sufficient surgical field exposure was obtained in all cases and all tumors were completely resected. No salivary fistula, earlobe, or temporal numbness occurred. The function of facial nerve was well preserved in addition to surgical needs to remove facial nerve branches. No tumor recurrence was found during 18-24months follow-up. All patients were satisfied with the appearance of incision. Conclusion: The anterior auricular extension incision is a feasible surgical approach in the resection of accessory parotid gland tumor, which can achieve good surgical exposure and surgical results. References | Related Articles | Metrics

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Prognostic Factors of Mucoepidermoid Carcinoma of Salivary Glands: a SEER Database-based Study ZHANG Shuguang, WANG Yulong, XU Wenguang, YIN Xiteng, HAN Wei, ZOU Huihui 2020, 36(10): 915-920.  DOI: 10.13701/j.cnki.kqyxyj.2020.10.007 Abstract ( 235 )   PDF (938KB) ( 272 )   Objective: To analyze and screen prognostic risk factors of salivary gland MEC. Methods: The clinicopathological data and follow-up data of salivary gland MEC patients were downloaded from the SEER (surveillance, epidemiology, and end results) database in the United States between 2004 and 2015. Patients were randomly divided into the training and validation cohorts. The MEC patients in the domestic database were selected as an independent external verification group. The univariate analysis was initially employed to screen the clinicopathological factors that affected the prognosis of MEC patients. The multivariate analysis was used to obtain independent factors affecting the prognosis of patients. The nomogram was constructed and the distinguishing ability and consistency of the model were tested. Results: A total of 2209 patients with MEC of salivary glands were included in this study. Patients were assigned as training set (n=1657), and the rest were selected as SEER validation set (n=552). An external validation was performed by a set of independent 234 MEC patients from Nanjing Stomatological Hospital and Fu Dan Hospital in China .The univariate analysis showed that age, gender, clinical stage, T stage, N stage, M stage, and histological grade neck dissection were important clinicopathological factors affecting the prognosis of patients. Multivariate analysis showed that age, T stage, N stage, M stage, histological grade, and neck dissection were independent factors affecting the prognosis of MEC patients of salivary glands. An individualized nomogram was successfully constructed to predict the prognosis of patients with MEC of salivary glands, and the discriminability test showed that the C-index was 0.794 (95%CI 0.744-0.844), indicating that the model reached a good discriminability. The consistency test showed that the predicted survival rate by the nomogram was consistent with the actual survival rate. Conclusion: Age, gender, clinical staging, T staging, N staging, M staging, histological grading, and neck dissection were the prognostic factors of MEC patients in salivary glands. The constructed nomogram could effectively predict the prognosis of patients with salivary gland MEC. References | Related Articles | Metrics

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Plumbagin Induces Tongue Squamous Cell Carcinoma Cells Apoptosis via ROS-mediated JNK Signaling Pathway ZHOU Qun, QIU Jiaxuan 2020, 36(10): 921-924.  DOI: 10.13701/j.cnki.kqyxyj.2020.10.008 Abstract ( 226 )   PDF (922KB) ( 246 )   Objective: To explore the underlying molecular mechanisms of plumbagin-induced apoptosis of tongue squamous cell carcinoma cells. Methods: The effect of plumbagin on cell proliferation was determined by CCK-8 assay. Cell apoptosis and the expression of cellular ROS generation were measured by flow cytometry. Protein levels of Bcl-2, Bax, and p-JNK were evaluated by Western blot. Results: CCK-8 assay showed that plumbagin significantly inhibited cell proliferation in a dose-dependent manner(P<0.001). The results of flow cytometer suggested that plumbagin markedly increased cell apoptosis and ROS generation. Western blot showed that the expression of Bax increased, while the expression of Bcl-2 decreased after plumbagin treatment. In a time-dependent manner, plumbagin up-regulated the expressions of p-JNK (P<0.05,P<0.01,P<0.001). However, the ROS inhibitor NAC significantly reversed the expression level of p-JNK and cell apoptosis(P<0.001,P<0.001). Conclusion: Plumbagin had excellent anti-proliferation effects on tongue squamous cell carcinoma cells. In addition, plumbagin induced cell apoptosis through ROS-mediated JNK pathway. References | Related Articles | Metrics

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Clinicopathological Analysis of Pleomorphic Hyalinizing Angiectatic Tumor of Soft Tissue SUN Yajing, LI Baojiang 2020, 36(10): 925-929.  DOI: 10.13701/j.cnki.kqyxyj.2020.10.009 Abstract ( 154 )   PDF (4234KB) ( 242 )   Objective: To investigate the clinicopathological features, diagnosis, and differential diagnosis of pleomorphic hyalinized angiectatic tumor of soft tissue. Methods: Pleomorphic hyalinized angiectatic tumors of soft tissue were analyzed and reviewed. Results: Tumors were nodular, which had clear boundaries with the surrounding tissue. Tumors' diolame was complete or incomplete. Under the microscope, thin-walled dilated vascular wall were seen, which had fibrinous substance deposition and thrombus. Between vessels pleomorphic tumor cells were seen. The mitotic figure was rare. Immunohistochemical staining showed CD34(+), Vimentin(+), BCL-2 (Partial+),and Ki-67 (+3%). Conclusion: Pleomorphic hyalinized angiectatic tumor of soft tissue is a rare tumor which has good prognosis. However, it needs to be identified with multiple tumors. Immunohistochemical markers may assist the diagnosis. References | Related Articles | Metrics

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Expression of CYP27B1 in Oral Squamous Cell Carcinoma and Its Relationship with Prognosis GUO Junfeng, ZHANG Gang, TAN Yinghui 2020, 36(10): 930-933.  DOI: 10.13701/j.cnki.kqyxyj.2020.10.010 Abstract ( 232 )   PDF (1829KB) ( 139 )   Objective: To investigate the expression of CYP27B1 in oral squamous cell carcinoma (OSCC) and its correlation with prognosis. Methods: The data of 372 patients with OSCC were downloaded from the TCGA (the cancer genome atlas) database as a TCGA database cohort to extract the CYP27B1 gene expression-related data of the above-mentioned patients. The differences of CYP27B1 expression in cancer and adjacent tissues of OSCC patients were calculated and analyzed using the edgR package. 146 patients with OSCC in the Department of Stomatology, Xinqiao Hospital (Second Affiliated Hospital of Army Military Medical University) were selected as a Xinqiao Hospital cohort. The paraffin sections of the patients were immunohistochemical stained, and the expression of CYP27B1 was detected. According to the CYP27B1 expression results, the median value of OSCC patients was divided into CYP27B1 high expression and CYP27B1 low expression group. Combined with the clinical medical records, the relationship between CYP27B1 expression and prognosis was analyzed. Results: CYP27B1 was expressed in OSCC tissues both in TCGA database cohort and Xinqiao Hospital cohort. Kaplan-meier survival analysis showed that the overall survival time (OS) of the group with high expression of CYP27B1 was significantly higher than that of the group with low expression (P<0.05). Conclusion: CYP27B1 poor expression may relate to the occurrence, development, and prognosis of OSCC. References | Related Articles | Metrics

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Odontogenic Myxoma: Report of 9 Cases WEI Yunpeng, ZHAO Sufeng 2020, 36(10): 934-937.  DOI: 10.13701/j.cnki.kqyxyj.2020.10.011 Abstract ( 283 )   PDF (2028KB) ( 365 )   Objective: To investigate the clinical features, the diagnostic points, the therapeutic methods, and the prognosis of odontogenic myxoma. Methods: 9 cases of odontogenic myxoma diagnosed through pathological examination from January of 2010 to December of 2019 were reviewed and analyzed by clinical features, imaging data, pathological characteristics, surgery methods, and prognosis. Results: The clinical symptoms of odontogenic myxoma were atypical, and the imaging data had reference significance for diagnosis. However, the definite diagnosis still depended on pathological examination. Surgical treatment was the first therapeutic scheme of odontogenic myxoma currently which should be combined with the clinical features of patients. References | Related Articles | Metrics

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Change of Upper Airway after Mandibular Setback by BSSRO in Patients with Skeletal Ⅲ Malocclusion LIU Gang, ZHANG Liang, LI Changshun 2020, 36(10): 938-941.  DOI: 10.13701/j.cnki.kqyxyj.2020.10.012 Abstract ( 168 )   PDF (1157KB) ( 145 )   Objective: To evaluate the change in pharyngeal airway space and the position of hyoid bone after mandibular setback in patients with skeletal Ⅲ malocclusion. Methods: 25 patients with skeletal Ⅲ malocclusion were collected from Jan. 2016 to Jun.2019 in Xuzhou Stomatological Hospital, in which male were 13 and female were 12. The mean age was 22.15. The bilateral sagittal split ramus osteotomy (BSSRO) was applied. Lateral cephalometric radiographs were taken before and after surgery. The changes of airway in soft palate, tongue, epiglottis region, and hyoid bone position were measured in sagittal direction. Results: After the operation, the soft palate, base of tongue, and posterior area of epiglottis were narrowed, and hyoid bone shifted backwards and downward in all patients. There was no significant statistical difference in upper airway between male and female, while hyoid bone position in males was lower than that in females. Conclusion: The study indicated that BSSRO made upper airway narrow in different degrees and hyoid bone shifting after operation. Obstructive sleep apnea-hypopnea syndrome would be caused by the great amount of mandibular setback References | Related Articles | Metrics

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Effect of Pulsed Nd:YAG Laser on Pregnancy-associated Pericoronitis and Dental Anxiety in Pregnant Women GONG Tianyuan, SHEN Yafeng, ZHANG Chaojie, Reisha·Abdukim, XU Jiang 2020, 36(10): 942-947.  DOI: 10.13701/j.cnki.kqyxyj.2020.10.013 Abstract ( 208 )   PDF (772KB) ( 223 )   Objective: To analyze the short-term efficacy of pulsed Nd:YAG laser-assisted treatment on pericoronitis during pregnancy and its effect on dental anxiety in pregnant women. Methods: 68 patients with mild pregnancy-associated pericoronitis during the alveolar surgery from September 2017 to May 2019 were collected and randomly divided into two groups (n=34) according to the order of treatment. The treatment period was one week. The non-laser group was given conventional pericoronal irrigation and the laser group was given adjuvant therapy with pulsed Nd:YAG laser. The pain degree (NRS), mouth opening, gingival index (GI), bleeding index (BI), MDAS score, total effective rate, recurrence rate, and pregnancy outcome were compared between two groups. Results: The mouth opening degree, NRS, GI, and BI of the laser group were improved compared with the control group on the 2nd-5th days (P<0.05), however, no difference on the 1st and 7th days (P>0.05). The number of DA in the laser group was lower than that in the non-laser group on the first time of treatment (P<0.01). After 1 week, the total effective rate of the laser group was greater than that of the non-laser group (P<0.05). After 6 months, the recurrence rate in the laser group was lower than that in the non-laser group (P<0.05). None of the 68 patients had adverse pregnancy outcome. Conclusion: Pulsed Nd:YAG laser-assisted treatment has significant short-term efficacy on mild pregnancy-associated pericoronitis. It is safety and can alleviate dental anxiety in pregnant women. References | Related Articles | Metrics

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Preparation of Decellularized Scaffolds from Submandibular Glands of Rats DAI Taiqiang, ZHANG Linlin, AN Ying, XU Fangfang, SHAO Xiaoxi, LIU Yanpu, LIU Bin 2020, 36(10): 948-952.  DOI: 10.13701/j.cnki.kqyxyj.2020.10.014 Abstract ( 184 )   PDF (4085KB) ( 275 )   Objective: To explore the method for preparation of decellularized scaffold (DS) from submandibular glands (SMGs) of rats and provide scaffolds for tissue engineering regeneration of salivary glands. Methods: Perfusion system of SMGs was built by ductal and venous cannula. Decellularized SMGs scaffolds were produced by freeze-thaw cycle and sequence perfusion with SDS, Triton X-100, and DNase I through the cannula of vein. HE staining and SEM were performed to observe the general and microstructure of the DS, while Masson staining and immunofluorescence staining were performed to assess extracellular matrix retention. At the same time, the casts of duct and vein for SMGs and DS were compared to analyze the retention of ductal and venous system. Finally, the retention of collagen and DNA was detected by quantitative analysis. Results: Perfusion system was constructed by ductal and venous cannula and DS was produced successfully by perfusion method. DS held the original structure and kept retention of collagen type Ⅰ, Ⅳ, fibronectin, and laminin in extracellular matrix. DNA quantification showed that less than 50 μg/g DNA remained on dry tissue. The casts suggested relatively intact ductal and venous system after decellularization. Conclusion: Decellularized salivary glands scaffolds can be successfully prepared by perfusion method and it will provide scaffolds for tissue engineering construction of salivary glands. References | Related Articles | Metrics

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Comparison of Oral Dexmedetomidine and Midazolam for Premedication in Children after Dental Procedures WANG Li, HUANG Lili, PENG Wei 2020, 36(10): 953-956.  DOI: 10.13701/j.cnki.kqyxyj.2020.10.015 Abstract ( 221 )   PDF (839KB) ( 220 )   Objective: To compare the effect of oral dexmedetomidine and oral midazolam on preoperative cooperation among children who underwent dental procedures. Methods: Sixty patients were equally randomized divided into two groups: midazolam group (n=30) and dexmedetomidine group (n=30). Midazolam was taken orally with 0.5 mg/kg. Dexmedetomidine was taken orally with 2 μg /kg. The changes of heart rate (HR), respiratory rate (RR), and blood oxygen saturation (SpO2) of the two groups were recorded before premedication and 10 min, 20 min, and 30 min after administration. Ramsay sedation rating scale was used to evaluate the sedation effect of two groups. The acceptance rate of face mask, the parental separation anxiety scale, and emergence delirium scale were observed. Results: At 10 min, 20 min, and 30 min after administration, there was no significant difference in HR, RR, and SpO2 between two groups (P>0.05). The Ramsay scores at 10 min, 20 min, and 30 min after premedication were not statistically different (P>0.05) in both groups. The rate of acceptance face mask and separation from parental were not statistically different (P>0.05) in both groups. The incidence of emergence delirium in dexmedetomidine group was lower than that of midazolam group (P<0.05). Conclusion: Dexmedetomidine is a safe and effective sedative agent for pediatric anesthetic premedication. References | Related Articles | Metrics

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Remodeling Pattern of Mandible after “All-on-4” Immediate Fixed Rehabilitation: A Radiographic Study JIA Shengnan, DI Ping, LIN Ye 2020, 36(10): 957-961.  DOI: 10.13701/j.cnki.kqyxyj.2020.10.016 Abstract ( 220 )   PDF (1148KB) ( 182 )   Objective: To investigate the pattern of mandible remodeling after “All-on-4” immediate fixed rehabilitation and its influential factors. Methods: 30 patients rehabilitated with “All-on-4” implant-supported fixed restorations were studied. All patients were followed-up for more than 1 year. Panoramic radiographs (OPT) were taken after the completion of prosthetic restoration and during the follow-up period. The samples were investigated by 2-dimensional radiological retrospective analysis. 7 sites on the mandible were marked (M: mid-line site; ML/MR: the sites between two implants on the same side; L1/R1: molar sites; L2/R2: retromolar sites) based on the anatomical landmarks and the locations of implants on the OPTs. The vertical bone heights were measured and the average annual bone resorption rates were calculated on these 7 sites. Correlation analysis was conducted for age, gender, initial mandibular height, and chewing force, respectively. Results: Bone resorption was observed on sites ML, M, and MR [ML:(-0.02±0.33) mm/year;M:(-0.15±0.27) mm/year;MR:(-0.02±0.22) mm/year]. Bone augment was discovered at L1/R1 [L1: (0.25±0.57) mm/year, R1: (0.21±0.61) mm/year]. While slight alveolar height increments were recorded at L2/R2 [L2: (0.07±0.580 mm/year, R2: (0.01±0.52) mm/year]. Furthermore, chewing force was positively correlated with the height changes at site L1/R1 (P<0.05). Conclusion: “All-on-4” immediate fixed rehabilitation may change the mandible remodeling pattern. Placing implants between the mental foramen contributes to the lower resorption rate in the area between mental foramen and fixed rehabilitation contributes to the increments of vertical bone height at the molar sites of the mandible. References | Related Articles | Metrics

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Effects of Ridge Preservation with Different Membrane Techniques after Extraction of Severe Periodontitis Related Teeth ZHANG Hexin, LI Xinyue 2020, 36(10): 962-967.  DOI: 10.13701/j.cnki.kqyxyj.2020.10.017 Abstract ( 268 )   PDF (3710KB) ( 329 )   Objective: To compare the clinical effects of different treatment techniques of ridge preservation following tooth extraction in patients with severe periodontitis. Methods: A total of 21 hopeless teeth diagnosed with severe periodontitis at our institution were included. Tooth extraction and ridge preservation were operated in each socket. All sockets were grafted with Bio-Oss after tooth extraction. They were divided into three groups by covering different membranes: Bio-Gide group, PRF group, and Bio-Gide+PRF group. Clinical and radiographic examinations were carried out pre operation, 3 and 6 months post-operation. Keratinized tissue width (KTW) and gingival recession (GR) of adjacent tooth were measured. Adjacent bone height (ABH) and socket depth (SD) were measured using parallel projection of apical radiographs. Buccal-lingual bone width (BLW) was measured using CBCT. Results: There was no significant difference at GR and ABH among three groups 6 months post-operation (P>0.05). Compared to pre-operative parameters, after 6 months, SD values of 3 groups had significant change (P＜0.05). Only BLW result of PRF group became worse after surgery (P<0.05). After surgery, the values of KTW from high to low were as follows: Bio-Oss+PRF group, Bio-Oss+Bio-Gide+PRF group, and Bio-Oss+Bio-Gide group. Conclusion: All techniques following tooth extraction in advanced periodontitis patients can effectively maintain the adjacent alveolar bone height and reduce the further recession of soft tissue. Covering Bio-Gide+PRF membrane after Bio-Oss grafting is more beneficial to maintain the width of buccal-lingual alveolar bone and has advantages of promoting soft tissue healing and preserving sufficient keratinized gingiva. References | Related Articles | Metrics

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Effect of In Vitro Indirect Co-culture of Rabbit Dental Pulp Stem Cells with Schwann Cells on Proliferation and Neuronal Differentiation of Rabbit Dental Pulp Stem Cells ZHANG Weijia, PAN Shuang, LI Yanping, HE Lina, WU Zuodong, NIU Yumei 2020, 36(10): 968-972.  DOI: 10.13701/j.cnki.kqyxyj.2020.10.018 Abstract ( 213 )   PDF (2626KB) ( 184 )   Objective: To study the effects of indirect co-culture of rabbit dental pulp stem cells (rDPSCs) and rabbit schwann cells (rSCs) in vitro on each other's proliferation and the possibility of rSCs inducing rDPSCs into neural differentiation. Methods: The indirect co-culture system of rSCs/rDPSCs was constructed by Transwell indirect co-culture system, and MTT method was adopted to detect the cells' proliferation. Immunofluorescence staining and Western bolt were used to study the expression of S-100β and NF-H and the possibility of rSCs to induce neural differentiation of rDPSCs. Results: When rDPSCs and rSCs were indirectly co-cultured in a 1∶2 ratio for 5 days, rDPSCs could facilitate the proliferation of rSCs (P<0.05), and rSCs also had obvious effect on the proliferation of rDPSCs (P<0.05). After indirect co-culturet with rSCs for 7 days and 14 days, rDPSCs became neuron-like cells, S-100β and NF-H were expressed and both presented a higher level after 14 days (P<0.01). Conclusion: In the indirect co-culture system of rDPSCs and rSCs, rDPSCs and rSCs can significantly promote each other's proliferation after 5 days, while rSCs can induce the neural differentiation of rDPSCs. References | Related Articles | Metrics

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Functions of Platelet-rich Fibrin on Proliferation and Osteogenic Differentiation of Dental Pulp Stem Cells YAN Na, HUANG Tao, ZHANG Zhongyue, ZHANG Shuhua, WANG Lihua, HAN Guoliang 2020, 36(10): 973-977.  DOI: 10.13701/j.cnki.kqyxyj.2020.10.019 Abstract ( 192 )   PDF (889KB) ( 170 )   Objective: To investigate the effects of platelet-rich fibrin on the proliferation and osteogenic differentiation of human dental pulp stem cells. Methods: Human dental pulp stem cells were treated with platelet-rich fibrin of different dilution concentrations, and the proliferative activity of human dental pulp stem cells was detected by MTT assay. Flow cytometry was used to detect the cell cycle. The expression level of bone differentiation markers ALP, COL-I, RUNX2, and OCN were measured by western blot. Results: When the concentration of platelet-rich fibrin reached more than 50%, the proliferation activity of human pulp stem cells was significantly enhanced, and more cells were induced to S phase. The expression of osteogenic differentiation markers ALP, COL-I, and RUNX2 were significantly up-regulated. When the concentration of platelet-rich fibrin reached 80%, the expression of OCN was also significantly increased. Conclusion: Platelet-rich fibrin can promote the proliferation and osteogenic differentiation of human dental pulp stem cells. References | Related Articles | Metrics

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New Strategy for Minimally Invasive Apical Microsurgery with Aid of Dynamic Navigation System XIA Juan, ZHANG Jieli, YE Zhitong, JIANG Qianzhou, YANG Xuechao 2020, 36(10): 978-982.  DOI: 10.13701/j.cnki.kqyxyj.2020.10.020 Abstract ( 321 )   PDF (4386KB) ( 353 )   Objective: To explore the new method for minimally invasive and accurate apical microsurgery with the aid of dynamic navigation systems. Methods: A patient suffered from chronic periapical periodontitis and failure of root canal therapy was selected. An open position cone beam computer tomography (CBCT) was made. Preoperative plan was made in IRIS software. The infrared lamp of navigation systems was matched and positioned before the surgery. Guided osteotomy and root resection were performed with the aid of dynamic navigation systems. Results: The periapical pathological tissue was removed accurately with minimal lesion to osseous and soft tissue. The minimally invasive and precise treatment was realized. The patient's satisfaction was improved. Conclusion: Minimally invasive apical surgery with the aid of dynamic navigation systems would realize preoperative simulation, visualization, and accurate and minimal invasion. It was simple, efficient, and would lower the technique sensitivity. References | Related Articles | Metrics