Effects of Extracts from “Red Complex” Periodontal Pathogens on Osteogenic Differentiation Capacity of Human Periodontal Ligament Cells

doi:10.13701/j.cnki.kqyxyj.2020.09.010

Abstract

Abstract: Objective: To evaluate the effects of extracts from “Red Complex” periodontal pathogens on osteogenic differentiation capacity of human periodontal ligament cells (hPDLCs). Methods: hPDLCs were isolated and primary cultured from first premolars extracted from orthodontic patients. The primary cultured cells were verified by immunofluorescence and osteogenic induction methods. The model of “Red Complex” was set by co-culturing Pg, Td, and Tf in vitro, and was verified by SEM. Soluble bacterial extracts obtained from “Red Complex” was used to investigate the effects of “Red Complex” periodontal pathogens on the ALP activities, osteogenic related genes expression, and mineralization of hPDLCs by performing ALP assay, RT-PCR, and Alizarin Red staining. Results: Under the stimulation of 100 μg/mL bacterial extracts, the ALP activities, expression of osteogenic related genes ALP, Runx2, OPN, and COL1, and mineralization of hPDLCs were significantly decreased. Conclusion: “Red Complex” periodontal pathogens inhibited the osteogenic differentiation capacity of hPDLCs.

Key words: periodontal ligament cells, periodontal pathogens, Red Complex, osteogenic differentiation

ZHOU Hejie, LI Shenghong, PENG Peizhao, TANG Rong, LI Ailian, ZENG Jin. Effects of Extracts from “Red Complex” Periodontal Pathogens on Osteogenic Differentiation Capacity of Human Periodontal Ligament Cells[J]. Journal of Oral Science Research, 2020, 36(9): 839-843.

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