Effects of Fas Ligand Overexpression on Osteogenic Differentiation Potential of Human PDLSCs

doi:10.13701/j.cnki.kqyxyj.2019.12.017

Abstract

Abstract: Objective: To investigate the effect of FasL overexpression on osteogenic differentiation of human periodontal ligament stem cells (PDLSCs). Methods: Human PDLSCs were cloned and cultured in vitro with limited dilution. FasL overexpression plasmid was constructed and transfected into PDLSCs. The experiment was divided into control group, no-load control group (NC group), and FasL overexpression group (EX group). Osteogenic was induced in each group. Alizarin red staining, real-time PCR, and western blot were used to detect the osteogenic differentiation ability of PDLSCs. Results: The osteogenic differentiation ability of EX group was significantly higher than that of control group and NC group (P<0.05). There was no significant difference between control group and NC group (P>0.05). Conclusion: FasL can promote the osteogenic differentiation of human PDLSCs.

Key words: FasL, periodontal ligament stem cells, osteogenic differentiation potential

MA Lingzhi, REN Yalan, QIAN Liping, GE Wenbin, LIU Yali, ZHOU Zhi. Effects of Fas Ligand Overexpression on Osteogenic Differentiation Potential of Human PDLSCs[J]. Journal of Oral Science Research, 2019, 35(12): 1169-1172.

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