Journal of Oral Science Research

Journal of Oral Science Research ›› 2016, Vol. 32 ›› Issue (11): 1165-1167.DOI: 10.13701/j.cnki.kqyxyj.2016.11.013

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Effect of Endothelin-1 on Expression of Tumor Necrosis Factor of Periodontal Ligament Stem Cells

LIANG Li1, YU Ji-feng2, ZHOU Wei1, LIU Hong-chen3*   

  1. 1. Department of Stomatology, the 309th Hospital of Chinese PLA, Beijing 100091, China;
    2. Department of Ophthalmology, Beijing Children's Hospital, Capital Medical University, Beijing 100000, China;
    3. Institute of Stomatological Research, Chinese PLA General Hospital, Beijing 100000, China
  • Received:2016-04-21 Online:2016-11-25 Published:2016-11-25

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Abstract: Objective: To investigate how endothelin-1 affects the expression of tumor necrosis factor (TNF-α) in periodontal ligament stem cells (PDLSCs). Methods: The PDLSCs were stimulated with endothelin-1 (1, 10, 100nmol/L) for 12, 24, and 72 hours. The untreated PDLSCs served as normal control. The effect of ET-1 stimulation on TNF-α protein levels was evaluated by ELISA and Western blot. Results: The TNF-α protein levels were enhanced by ET-1 stimulation in a dose-dependent and time-course manner. Conclusion: These results indicate that ET-1 is involved in the regulation of TNF-α expression in PDLSCs and suggest that ET-1 signaling may be a therapeutic target for treating TNF-α-dependent inflammatory responses.

Key words: Periodontitis, Periodontal ligament stem cells, Endothelin-1, Tumor necrosis factor

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