A Study on Glucose-sensitive Hydrogel in the Treatment of Periodontitis in Diabetic Rats

doi:10.13701/j.cnki.kqyxyj.2020.06.015

Abstract

Abstract: Objective: To prepare glucose-sensitive controlled-release hydrogel for the treatment of periodontitis in diabetic rats models. Methods: Chitosan was used as matrix material to prepare hydrogel. Glucose oxidase was immobilized on the hydrogel to prepare glucose sensitive hydrogel, which was characterized by stability test and cell adhesion test. Metronidazole-loaded hydrogel was immersed in dynamic changing concentration of glucose solution. Drug release test was used to test its ability to respond to blood glucose. Finally, Hydrogel was put into the experimental periodontal pocket of diabetic rats to observe its therapeutic effects. Results: The photo-crosslinked hydrogel had stable mechanical properties and facilitated the adherence of osteoblasts MC3T3-E1. After glucose oxidase was immobilized on the surface of the hydrogel, metronidazole release was changed with different concentration of glucose. In the in vivo study, the swelling and bleeding of periodontal tissue were alleviated. Conclusion: A novel glucose sensitive hydrogel drug delivery system is prepared in this study, which provides a promising method for the treatment of diabetic periodontitis.

Key words: glucose response, chitosan, periodontitis, drug delivery

LIU Junyu, XIAO Yu, WANG Xiangyu, LIU Yan, LI Yufang, LIU Haifeng, CHEN Yu, BAO Chongyun. A Study on Glucose-sensitive Hydrogel in the Treatment of Periodontitis in Diabetic Rats[J]. Journal of Oral Science Research, 2020, 36(6): 567-571.

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