Effect of TNF-α on Expression of ephrinB2/EphB4 in Periodontal Fibroblasts

doi:10.13701/j.cnki.kqyxyj.2020.08.009

Abstract

Abstract: Objective: To investigate the effects of TNF-α on the expression of ephrinB2/EphB4 mRNA and protein levels in periodontal fibroblasts. Methods: Healthy teeth which need to be extracted due to orthodontic treatment were collected and the primary periodontal fibroblasts were cultured. The expressions of ephrinB2/EphB4 mRNA and protein levels were detected after the periodontal ligament fibroblasts were stimulated with different concentrations of TNF-α after 24 h and 48 h. Results: The mRNA concentrations of ephrinB2 and EphB4 were both decreased after 24 h (P<0.05). After 48 h, the low concentration group (0.1 μg/L-1 μg/L) had no effect on ephrinB2 expression. 0.1 μg/L could increase the expression of EphB4. However, the high concentration could decrease the expression of ephrinB2/ EphB4. Conclusion: ephrinB2/EphB4 was decreased by TNF-α after 24 h, however, EphB4 expression was increased after 48 h, which was implied to be a reference to study the bone remodeling in low-concentration inflammatory state.

Key words: tumor necrosis factor alpha, periodontal ligament fibroblasts, ephrinB2, EPHB4, tooth movement, periodontitis

WANG Linxuan, WANG Qi, ZHAO Yun, LIU Yiming, HAN Mei, MI Fanglin. Effect of TNF-α on Expression of ephrinB2/EphB4 in Periodontal Fibroblasts[J]. Journal of Oral Science Research, 2020, 36(8): 741-744.

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