An Experimental Study of Multilayer Ligamental Periodontal Cell Sheets on Tissue Regeneration

doi:10.13701/j.cnki.kqyxyj.2016.11.012

Abstract

Abstract: Objective: To fabricate multilayer periodontal ligament stem cell pellets (MUCPs) and monolayer periodontal ligament stem cell pellets (MCPs), and to evaluate their biological activity and regeneration potential. Methods: Biological properties of two kinds of cell sheets were observed by immunohistochemistry and scanning electron microscopy. And then the cell sheets were combined with CBB to construct biological roots. Tissue regeneration in the nude mice after ectopic transplantation was observed. Results: The in vitro experiments showed higher levels of structural proteins in the extracellular matrix composed of COL-I, COL-III, fibronectin and laminin in the MUCPs. Upon transplantation, formation of mineralized nodules and periodontal fibrous tissue similar to the periodontal tissues was more obvious in the MUCPs than that in MCPs in vivo. Conclusion: MUCPs presented a good biological activity and it may be a promising alternative in periodontal repair for future clinical application.

Key words: Ligamental periodontal stem cell, Multilayer cell sheet, Monolayer cell sheet, Tissue regeneration, Microenvironment

CLC Number:

R781.4

NG Xue-peng, HE Yong, WANG Chong, LI Shi-ting, JIN Yan. An Experimental Study of Multilayer Ligamental Periodontal Cell Sheets on Tissue Regeneration[J]. Journal of Oral Science Research, 2016, 32(11): 1160-1164.

References

[1] Flores MG, Yashiro R, Washio K, et al. Periodontal ligament cell sheet promotes periodontal regeneration in athymic rats [J]. Clin Periodontol, 2008, 35(12)∶1066-1072
[2] Lin Y, Li S, Cao P, et al.The eFFects oF recombinant human GH on promoting tumor growth depend on the expression oF GH receptor in vivo [J]. Endocrinol, 2011, 211(3)∶249-256
[3] 李颖,张文元,杨亚冬,等.人牙周膜细胞在矿化液作用下的增殖、成骨分化与相关基因表达[J].口腔医学研究,2014,30(1)∶48-52
[4] 毛剑.干细胞和口腔医学的未来[J].中华口腔医学杂志,2009,44(1)∶23-26
[5] Guo W, Wang S, Yu X, et al. Construction oF a 3D collagen hybrid scaffoement oF the neural differentiation oF mesenchymal stem cells [J]. Nanoscale, 2016, 8(4)∶1897-1904
[6] 郭津源,仲维剑,柴松岭,等.牙齿煅烧颗粒结合富血小板纤维蛋白修复骨缺损的实验研究[J].口腔医学研究,2015, 31(11)∶1069-1072
[7] Guo weihua, Tang xue peng. Scaffold-free cell pellet transplantations can be applied to periodontal regeneration [J]. Cell Transplant,2014,23(2)∶181-194
[8] Kawanishi K, Nitta K. Cell sheet-based tissue engineering for mesothelial cell injury [J]. Contrib Nephrol,2015,185(12)∶66-75
[9] Shimizu T, Sekine H, Yang J, et al. Polysurgery of cell sheet grafts overcomes diffusion limits to produce thick, vascularized myocardial tissues [J]. Tissue Engineering Part A, 2006, 20(6)∶708-710
[10] Singhatanadgit, W, N Donos, I Olsen. Isolation and characterization of stem cell clones from adult human ligament [J]. Tissue Engineering Part A, 2009, 15(9)∶2625-2636
[11] Lin NH, Gronthos S, Mark Bartold P. Stem cells and future periodontal regeneration [J]. Periodontol,2009,27(6)∶51-63
[12] Feres M, Gursky LC, Faveri M, et al. Clinical and microbiological benefits of strict supragingival plaque control as part of the active phase of periodontal therapy [J]. J Clin Periodontol,2009,36(10)∶857-867
[13] M Kawamura, S Miyagawa, S Fukushima. Xenotransplantation of Bone Marrow-Derived Human Mesenchymal Stem Cell Sheets Attenuates Left Ventricular Remodeling in a Porcine Ischemic Cardiomyopathy Model [J]. Tissue Eng Part A, 2015, 21(15)∶2272-2280
[14] Intini G. Future approaches in periodontal regeneration: gene therapy, stem cells, and RNA interference [J]. Dent Clin North Am,2010,54(1)∶141-155
[15] Aschos NK, Brown WE, Eswaramoorthy, et al. Advances in tissue engineering through stem cell-based co-culture [J]. TissueEng regen Med,2015,9(5)∶488-503