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    25 November 2016, Volume 32 Issue 11 Previous Issue    Next Issue

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    Histological Observation of Peri-implant Soft Tissues to Different Implant Abutment Materials under Inflammatory Condition: a Study in Vivo
    WANG Min, YANG Hui, WANG Yi-ning, XIA Hai-bin, ZOU Hai-xiao
    2016, 32(11): 1113-1116.  DOI: 10.13701/j.cnki.kqyxyj.2016.11.001
    Abstract ( 336 )   PDF (1020KB) ( 260 )  
    Objective: To observe the histological response of peri-implant soft tissues to different implant abutments materials with or without peri-implant mucotitis. Methods: The third and fourth premolars and the first molar of three beagle dogs were extracted and eight implants were placed into each dog after healing. Two months later, the titanium and zirconium healing abutments were connected, plaque index, gingival index and probing depth were recorded as the initial time. Then ligaments were placed around the neck of healing abutments to induce peri-implant mucotitis. Plaque index, gingival index and probing depth were recorded again. The soft tissues were harvested and histological observation was performed to evaluate the inflammatory cells infiltration around titanium and zirconium healing abutments. Results: There were no differences on plaque index and probing depth of soft tissues around titanium and zirconium healing abutments with peri-implantitis, but gingival index was significantly higher with peri-implantitis. The HE staining showed that the inflammatory cells were located in the soft tissues around healing abutments with peri-implantitis. The immunohistochemistry revealed that inflammatory cells were expressed at the basement memberane zone, soft tissues around titanium and zirconium healing abutments, and endothelial cells of vessels in the vicinity. Conclusion: Histological responses of peri-implant soft tissues to titanium and zirconium healing abutments were similar under inflammatory condition.
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    Expression of Amelogenin and Ameloblastin in the Molar Tooth Germs of MMP-9 Knock-out Mice
    LI Tong, YUAN Guo-hua, NIE Min, YANG Guo-bin
    2016, 32(11): 1117-1121.  DOI: 10.13701/j.cnki.kqyxyj.2016.11.002
    Abstract ( 332 )   PDF (1215KB) ( 366 )  
    Objective: To detect the expressions of Amelogenin and Ameloblastin in wild-type mice and MMP-9 gene knock-out mice (MMP-9-/-). Methods: The expression levels of Amelogenin and Ameloblastin in tooth germs were detected with immunohistochemistry in wild-type and MMP-9-/- mice at post-natal day 2 (P2), 5 (P5), and 7 (P7). Results: At P2, Amelogenin was mainly expressed in matured ameloblasts and the underlying odontoblasts. However, in MMP-9-/- mice, the expression of amelogenin was expanded to the dental mesenchyme. No difference was found in the expression pattern of Amelogenin between wild-type mice and MMP-9-/- mice at P5 and P7. However, the expression level of Amelogenin in ameloblasts was highly increased in MMP-9-/- mice compared with that in wild-type mice. No obvious differences were detected in the expression pattern or expression level of Ameloblastin between wild-type mice and MMP-9-/- mice at P2, P5, and P7. Conclusion: MMP-9 might be able to degrade Amelogenin, and further influence the expression pattern of Amelogenin.
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    Effects of Gingipains on the Expression of Bacteriostatic Cytokines in M1 Polarized Mouse Macrophages
    HOU Yu-bo, LIU Xin-chan, LI Ge-ge, PAN Jia-hui, TANG Qiu-ling, YU Wei-xian
    2016, 32(11): 1122-1126.  DOI: 10.13701/j.cnki.kqyxyj.2016.11.003
    Abstract ( 288 )   PDF (1064KB) ( 423 )  
    Objective: To explore the role of gingipain on bacteriostatic cytokines induced by M1 macrophages. Methods: The gingipain was directly extracted from the supernatant of Pg ATCC 33277 and identified by Mass Spectrography. The activity of the gingipain was determined by chromogenic substrate BAPNA. The Chromogenic Limulus Amebocyte Lysate endpoint assay with diazo-coupling reagents test was undertaken to detect the presence of gram-negative bacterial LPS contamination of the purified gingipain. The effects of gingipain on the expression of bacteriostatic cytokines was evaluated in M1 macrophages induced by Escherichia coli lipopolysaccharide (Ec-LPS), including three groups: the negative control group, Ec-LPS group and Ec-LPS+gingipain group. Expressions of IL-12, iNOS and IL-10q were detected by RT-PCR and ELISA in M1 macrophages. Results: The gingipain was characterized as RgpA, the enzymatic activity of gingipain was 20U/L, and the residue of LPS from gingipain was <0.01EU/ml. qRT-PCR and ELISA assays demonstrated that Ec-LPS induced high level of IL-12 and iNOS in RAW264.7, but not IL-10, compared with the control group (P<0.01), which indicated that the M1 macrophages were induced successfully. The gingipain significantly inhibited the level of IL-12 and iNOS in the Ec-LPS treated RAW264.7, but not IL-10, compared with the Ec-LPS group (P<0.01). Conclusion: Gingipain could suppress the bacteriostatic cytokines of IL-12 and iNOS from M1 macrophages induced by Ec-LPS.
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    Research of Pure Magnesium Micro-arc Oxidation-NaOH-silane Silk Fibroin Composited Coatings Implanted in Vivo
    LIU Ji-guang, WANG Zan, LIU Miao, LI Mu-qin
    2016, 32(11): 1127-1131.  DOI: 10.13701/j.cnki.kqyxyj.2016.11.004
    Abstract ( 242 )   PDF (1215KB) ( 205 )  
    Objective: To study the effects of ultrasonic micro arc oxidation silane coupled silk fibroin composite coating on the bone consolidation and degradation of pure magnesium implants. Methods: After ultrasonic micro arc oxidation, pure magnesium implants were treated with NaOH and then with silane coupled silk fibroin on the surface for 0.5 h or 1.5 h, which were divided into group A, group B and group C, respectively. The modified materials were implanted in the rabbit mandible. Results: Scanning electron microscope observation showed that the surface of group C was the most compact. The concentration of magnesium in blood showed little change after compound modification. Torsion experiment demonstrated that the group C > group B > group A, showing statistical difference. HE staining, immunohistochemistry and gray value analysis showed that the group C had the strongest ability of bone consolidation. Conclusion: Pure magnesium implant with ultrasonic micro arc oxidation of alkali (NaOH) processing and silane coupling and silk fibroin composite modification showed better degradation rate and stronger promotion capability of osseointegration.
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    Expression of Caveolin-1 in Mouse Bone Marrow Mesenchymal Stem Cells during Osteogenic Differentiation
    YANG Yang, ZHANG Gang
    2016, 32(11): 1132-1136.  DOI: 10.13701/j.cnki.kqyxyj.2016.11.005
    Abstract ( 220 )   PDF (1032KB) ( 245 )  
    Objective: To investigate the expression of Caveolin-1 in mouse bone marrow mesenchymal stem cells (BMSCs) during osteoblast differentiation. Methods: The mouse BMSCs were cultured in vitro and divided into osteoblast-induced groups and non-induced group. In the different periods (3, 7, 10 and 14 days), the osteoblast differentiation was identified by alkaline phosphatase (AKP) activity and alizarin red staining and the expression of Caveolin-1 mRNA and protein in BMSCs were detected by real-time RT-PCR and Western blot. Results: RT-PCR results showed that osteoblast-induced groups had a higher expression level of Caveolin-1 than non-induced group at the same periods, and the Caveolin-1 mRNA expression was increased in a time-dependent effect in the osteoblast-induced groups. Western blot results showed that osteoblast-induced groups also had a higher expression level of Caveolin-1 than non-induced group at the same periods. The Caveolin-1 protein expression was increased in a time-dependent effect in osteoblast-induced groups. Conclusion: In this study, the Caveolin-1 expression at both mRNA and protein levels in BMSCs were increased with the osteogenic differentiation. Caveolin-1 may be regulated the BMSCs osteogenic differentiation process.
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    Effect of Calcitonin Gene-related Peptide on OPN Expression in Osteoblasts
    LI Yuan-jing, YANG Lan, LI Qian-qian, SUN Qian-yue, DENG Tian, REN Wen, WU Cai-juan, GUO Lv-hua
    2016, 32(11): 1137-1140.  DOI: 10.13701/j.cnki.kqyxyj.2016.11.006
    Abstract ( 262 )   PDF (982KB) ( 377 )  
    Objective: To investigate the effects of Calcitonin gene related peptide on OPN expression in primary cultured rat osteoblasts. Methods: Primary cultured rat osteoblasts were obtained by trypsin and collagenase digestion way from calvarial bone of neonate rats and were identified by ALP staining and alizarin red staining. The appropriate concentration of CGRP was selected by CCK-8 assay.The expression of OPN were quantified by qPCR and Western blot after cultured with CGRP. Results: The proliferation activity of osteoblasts enhanced when the concentration of CGRP was 10-9mol/L. The expression of OPN mRNA increased on the third day(P<0.05), and the expression of OPN protein was consistent with the change of the OPN mRNA. Conclusion: CGRPcould promote the proliferation of osteoblasts when the concentration of CGRP was 10-9mol/L. CGRP could promote OPN expression in the primary cultured rat osteoblasts.
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    Effect of P120ctn Silence and Overexpression on Cell Migration and Invasion of Oral Squamous Cell Carcinoma
    CHEN Zhong, JI Qing, ZHENG Xiao-dan
    2016, 32(11): 1141-1145.  DOI: 10.13701/j.cnki.kqyxyj.2016.11.007
    Abstract ( 243 )   PDF (1367KB) ( 295 )  
    Objective: To investigate the effect of P120ctn silence and overexpression on cell migration and invasion of oral squamous cell cancer (OSCC). Methods: Plasmid pGFP-V-RS-P120ctn shRNA was used to transfect HN4 cells, and plasmid pCMV6-AC-GFP-P120ctn was used to transfect HN12 cells. Real-time PCR and Western Blot were performed to detect the mRNA and protein expression levels of P120ctn and E-cad in OSCC cells before and after transfection. Trans-well assays were used to detect the invasion and migration abilities of OSCC cells before and after transfection. Results: After plasmid pGFP-V-RS-P120ctn shRNA transfection in HN4 cells, the expression levels of P120ctn and E-cad were significantly decreased, while cell migration and invasion abilities were significantly increased (P<0.05). After plasmid pCMV6-AC-GFP-P120ctn transfection in HN12 cells, the expression levels of P120ctn and E-cad were significantly increased, while cell migration and invasion abilities were significantly decreased (P<0.05). Conclusion: P120ctn may be involved in the invasion and metastasis by regulating the expression of E-cad and cell adhesion in OSCC.
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    Effect of Cola Type Carbonated Beverage on Adhesive Strength of ZrO2 Restorations and Tooth
    ZHANG Jing-yu, DENG Jiu-peng, BAI Yu-hong, ZHOU Jian-ying, QI Yue-ming, QI Meng-chun
    2016, 32(11): 1146-1149.  DOI: 10.13701/j.cnki.kqyxyj.2016.11.008
    Abstract ( 289 )   PDF (1045KB) ( 337 )  
    Objective: To investigate the effect of carbonated drinks on the adhesive strength of all-ceramic restorations and tooth. Methods: Forty-five premolars were collected and were divided into 3 groups. Group A was 3M RelyX luting group, Group B was 3M ESPE RelyX Veneer group and Group C was PULPDENT embrace group. The teeth were all bonded to ZrO2 block with different cements respectively. After curing for four minutes, the specimens were soaked into saline. After curing for 5 minutes and 24 hours, five specimens of each group were soaked in cola for five minutes. For the next 3 months, immersed into cola 2 times a day with an interval of 12 hours and the samples were preserved in saline preservation for the rest of the time. In each group, the other five specimens were immersed in physiological saline as control. A shear strength test and SEM observation were performed after three months. Results: The group bond strength of Group A was weaker than those of Group B and Group C (P<0.05). There was no significant difference between Group B and Group C. In Group A, the bonding strength of 5 minutes group was lower than that in the group of 24 hours, and both were lower than that of the control group (P<0.05). In Group B, there was no difference between 5 minutes group and 24 hours group, and both were weaker than that of the control group. In Group C, the bonding strength of 5 minutes group was lower than that of the control group, but there was no difference between 24 hours group and the control group. Conclusion: The strength between the 3M RelyX luting and teeth were reduced when the specimens were immersed into cola, but the shear strength in 3M ESPE RelyX Veneer and PULPDENT Embrace was not significantly influenced by cola. With the extension of curing time, the effect of cola on 3M RelyX luting was gradually decreased. The influence of cola on 3M ESPE RelyX Veneer was limited. The influence of cola on PULPDENT embrace was the lowest after curing for 24 hours.
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    Preliminary Study on K2O-Na2O-Al2O3-SiO2 System Dental Glass Ceramics Matching Al2O3 Ceramics
    Feng-chao, WU Qiqi, XU Yu-jing, ZHANG Biao
    2016, 32(11): 1150-1152.  DOI: 10.13701/j.cnki.kqyxyj.2016.11.009
    Abstract ( 278 )   PDF (799KB) ( 263 )  
    Objective: Research the thermal treatment schedule of K2O-Na2O-Al2O3-SiO2 system dental glass ceramics matching Al2O3 ceramics. Methods: Select and analysis the raw material according to K2O-Na2O-Al2O3-SiO2 system ,produce glass - Al2O3 composite ceramic material. The products were analyzed by polaring microscope and X-ray diffractometer, the compressive strength was tested by material machine. Results: Leucite microcrystal was about 1.0μm, distributed in the glass matrix evenly; The compressive strength was about 460MPa, even the interface of glass combined with Al2O3 ceramics need in-depth exploration. Conclusion: K2O-Na2O-Al2O3-SiO2 system dental glass ceramics can be sintered and match Al2O3 ceramics with special thermal treatment schedule.
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    Expression of IL-6 in Periodontal Tissue during Orthodontic Relapse in Rats
    GAO Rui, AN Jing-tao, SONG Bing, SHAO Ping
    2016, 32(11): 1153-1155.  DOI: 10.13701/j.cnki.kqyxyj.2016.11.010
    Abstract ( 271 )   PDF (921KB) ( 262 )  
    Objective: To study the expression of inflammatory cytokines interleukin IL-6 in rat periodontal tissues during orthodontic relapse. Methods: Thirty male Wistar rats were randomly divided into normal control group (6) and orthodontic tooth movement model group (24). Devices of the model group were removed after 14 days with rats randomly divided into model-0 day, model-7 day, model-14 day, and model-21 day group. Animals were sacrificed on day 0, day 7, day 14 and day 21, respectively. Then, periodontal tissues were obtained to detect the changes of inflammatory cytokines IL-6 in protein and gene expression levels. Results: Compared with the control group, protein and gene expression levels of IL-6 in Model-0 day group did not change significantly (P>0.05). But the expression level of IL-6 protein and gene on Model-7 day increased significantly and reached the highest (P<0.05). The expression level of IL-6 protein and gene on Model-14 day was higher than the control group, but a little lower than the Model-7 day. The expression level of IL-6 protein and gene on Model-21 day significantly decreased when compared to the Model-7 day, and was close to the control group (P>0.05). Conclusion: The expression levels of IL-6 protein and gene significantly increased in the condition of rat orthodontic relapse, arrived the top level on Model-14 day, and then decreased gradually.
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    Prediction the Color of Tooth: A Method Based on Linear Regression
    YU Hao, ZHANG Dong, CHENG Shao-long, CHENG Hui
    2016, 32(11): 1156-1159.  DOI: 10.13701/j.cnki.kqyxyj.2016.11.011
    Abstract ( 320 )   PDF (887KB) ( 294 )  
    Objective: To establish a linear regression model for shade matching based on machine learning algorithms. Methods: One hundred and twenty-three undergraduates were recruited as the volunteers of the present study(mean age: 22.48±1.77 years old, male: female=64:59). The chromatic valuesof volunteer’s maxillary teeth (15-25) were measured with a spectrophotometer. The matrix of chromatic value (X) and regression coefficient (θ) were determined based on the data from the volunteers. Efforts have then been put into the optimizing evaluation function through collaborative filtering. Finally, a multiple linear regression model was established with the purpose to predict the chromatic value of missing tooth. Twenty-four volunteers at similar ages were sampled to evaluate the prediction accuracy of the multiple linear regression model. Thepredicted chromatic value of their teeth were calculated and compared to the measured values based on the spectrophotometer. Results: The prediction accuracy of the model on L*value was more than 80% among 94.17% of 240 teeth, while the prediction accuracy ona*value and b*value were more than 80% among the entire sampling teeth. Conclusion: The multiple linear regression model might be a suitable tool to predict the color of tooth in permanent dentition.
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    An Experimental Study of Multilayer Ligamental Periodontal Cell Sheets on Tissue Regeneration
    NG Xue-peng, HE Yong, WANG Chong, LI Shi-ting, JIN Yan
    2016, 32(11): 1160-1164.  DOI: 10.13701/j.cnki.kqyxyj.2016.11.012
    Abstract ( 204 )   PDF (1100KB) ( 259 )  
    Objective: To fabricate multilayer periodontal ligament stem cell pellets (MUCPs) and monolayer periodontal ligament stem cell pellets (MCPs), and to evaluate their biological activity and regeneration potential. Methods: Biological properties of two kinds of cell sheets were observed by immunohistochemistry and scanning electron microscopy. And then the cell sheets were combined with CBB to construct biological roots. Tissue regeneration in the nude mice after ectopic transplantation was observed. Results: The in vitro experiments showed higher levels of structural proteins in the extracellular matrix composed of COL-I, COL-III, fibronectin and laminin in the MUCPs. Upon transplantation, formation of mineralized nodules and periodontal fibrous tissue similar to the periodontal tissues was more obvious in the MUCPs than that in MCPs in vivo. Conclusion: MUCPs presented a good biological activity and it may be a promising alternative in periodontal repair for future clinical application.
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    Effect of Endothelin-1 on Expression of Tumor Necrosis Factor of Periodontal Ligament Stem Cells
    LIANG Li, YU Ji-feng, ZHOU Wei, LIU Hong-chen
    2016, 32(11): 1165-1167.  DOI: 10.13701/j.cnki.kqyxyj.2016.11.013
    Abstract ( 185 )   PDF (1127KB) ( 165 )  
    Objective: To investigate how endothelin-1 affects the expression of tumor necrosis factor (TNF-α) in periodontal ligament stem cells (PDLSCs). Methods: The PDLSCs were stimulated with endothelin-1 (1, 10, 100nmol/L) for 12, 24, and 72 hours. The untreated PDLSCs served as normal control. The effect of ET-1 stimulation on TNF-α protein levels was evaluated by ELISA and Western blot. Results: The TNF-α protein levels were enhanced by ET-1 stimulation in a dose-dependent and time-course manner. Conclusion: These results indicate that ET-1 is involved in the regulation of TNF-α expression in PDLSCs and suggest that ET-1 signaling may be a therapeutic target for treating TNF-α-dependent inflammatory responses.
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    Applied Anatomic Study on the Design and Vascular Net of Retroauricular Fascial Periosteum Flap
    PAN Xiao-meng, HUANG Xie-shan, YU Hai, JIAN Xin-chun, SHI Xiao-tian, LIU De-yu, CHEN Yao-hui, BAI Zhen-yu, HAN Jingxin
    2016, 32(11): 1168-1170.  DOI: 10.13701/j.cnki.kqyxyj.2016.11.014
    Abstract ( 190 )   PDF (916KB) ( 195 )  
    Objective: To measure the length, width and distribution of posteriorauricular artery (PAA) for clinical application. Methods: Three skull specimens for ascular cast were selected, one of which was perfused with PVA-Bio and photographed by X ray. Results: The average max-length of PAA's was 12.54 cm (11.54-13.70cm). The average max-width of PAA was 6.74 cm (5.54-7.93cm). In the skull specimen perfused with PVA-Bio, the average max-length of PAA was 7.84 cm (7.72-7.95cm) and the average max-length of PAA was 4.57 cm (4.28-4.85cm). Conclusion: Clinically, the largest area of retroauricular fascial periosteum flap based on PAA is 12.54cm×6.74cm.
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    Research of Cervical Abnormalities in Patients with Different Level of Deep Overbite
    YANG Xiao-feng, LIU Yi
    2016, 32(11): 1171-1175.  DOI: 10.13701/j.cnki.kqyxyj.2016.11.015
    Abstract ( 263 )   PDF (887KB) ( 509 )  
    Objective: To observe the incidences of cervical abnormalities in patients with different level of deep overbite and the relationship between the craniofacial morphology and the incidences. Methods: The incidences of cervical abnormalities in 124 patients with deep overbite (classified into 3 groups based on different level of deep overbite) and 48 subjects with neutral occlusion were counted, and the lateral cephalometric radiographs were measured and analyzed. Correlation analysis was used to analyze the relationship between cervical abnormalities and measurements. Results: The incidences of normal cervical, fusion anomalies and more than one deviation in the 4 groups were statistically different. There were statistical differences in PP-MP, SN-MP, SNB, ANB, A-N-Pog, ILs-PP, overjet and overbite among the 4 groups. The cervical abnormalities were correlated with PP-MP, ANB, A-N-Pog, ILs-PP, overjet and overbite. Conclusion: The incidences of fusion anomalies and more than one deviation increased with the level of deep overbite. The cervical abnormalities were correlated with mandibular position, vertical jaw relationship, overjet and overbite.
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    Clinical Characteristics and Outcomes of Epulis: 61 Cases Report
    LI Hong-mei, WANG Chun, DONG Wei-li
    2016, 32(11): 1176-1178.  DOI: 10.13701/j.cnki.kqyxyj.2016.11.016
    Abstract ( 262 )   PDF (909KB) ( 530 )  
    Objective: To investigate the clinical characteristics and outcomes of epulis treated with modified epulis resection. Methods: Sixty-one patients with epulis were collected and treated with modified epulis resection, clinical characteristics and outcomes were recorded and analyzed. Results: The mean age of patients was 33.2 years. It was often occurred in female at 20-29 years old and affected labial region of anterior teeth: fibrous epulis (49.2%), granuloma epulis(29.5%), and vascular epulis(21.3%). Only one case was relapsed after 1, 3 and 6 months follow up. Conclusion: Preoperative routine periodontal treatment to improve oral hygiene and increasing the keratinized gingiva by relaxing incision and repositioned flap contribute to restore the contour of gingiva and maintain long-term effect.
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    Clinical Analysis of 31 Cases of Temporomandibular Joint Ankylosis Secondary to Condylar Sagittal Fractures
    XIANG Guo-lin, DENG Mo-hong, LI Wei, WANG Lin-lin, REN Wen, HAN Qian-chao, LONG Xing
    2016, 32(11): 1179-1182.  DOI: 10.13701/j.cnki.kqyxyj.2016.11.017
    Abstract ( 207 )   PDF (861KB) ( 312 )  
    Objective: To explore the clinical characteristics of traumatic temporomandibular joint ankylosis (TMJA) secondary to condylar sagittal fractures. Methods: Thirty-one patients with a total number of 48 ankylosed temporomandibular joints secondary to condylar sagittal fractures, in the Hospital of Stomatology, Wuhan University during the period of 2001-2010, were retrospectively reviewed. Results: The mean age of patients at injury was 15.6 years old, and 23 patients (74.2%) was less than 16 years old. The time interval between initial injury and presentation of TMJA varied from 3 months to 20 years, with a mean disease course of 6.5 years. The articular discs were observed displaced or disrupted in all 48 ankylosed joints. Histopathology of ankylosed joint revealed that the medially displaced condylar fragments showed the changes of disusatrophy, while cartilaginous metaplasia and ossification were found in the lateral osseous fusions and hyaline degeneration was observed in the disrupted articular discs. Conclusion: Traumatic TMJA secondary to condylar sagittal fractures usually occurred in the adolescents. The damaged articular disc should be account for the development of TMJA. Furthermore, the osseous fusions were initially originated from the lateral of joint surface. Chondrification and endochondral ossification were responsible for the formation of osseous fusions.
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    Complications of Surgical Treatment of Mandibular Condylar Fractures
    ZHANG Ling-ge, LONG Xing, DENG Mo-hong, CAI Heng-xing, MENG Qing-gong
    2016, 32(11): 1183-1187.  DOI: 10.13701/j.cnki.kqyxyj.2016.11.018
    Abstract ( 255 )   PDF (839KB) ( 532 )  
    Objective: To explore the complications of condylar fractures after surgical treatment and the preventive measures. Methods: One hundred and forty-six fractured mandibular condyles of 116 patients were treated with open surgery. The parameters for postoperative evaluation were as follows: mouth-opening, mandibular deviation during mouth opening, masticatory function, facial nerve injury and surgical scars. Preoperative, postoperative, and follow-up radiographs were analyzed in the computer. Follow-up time range varied from 3 months to 20 years. Results: Of all the 116 patients, 86 were treated by open reduction and rigid internal fixation (ORIF) and the rest 30 patients were treated by removal of the fractured mandibular condylar fragments without fixation. In the ORIF group, surgical approaches and fixed methods were closely related with the occurrence of complications. Complications occurred in the two groups included temporomandibular disorders (TMD), osteonecrosis, temporary or permanent facial nerve injury, visible scars, malocclusion, deviation of the mandible, facial asymmetry, restricted mandibular movement, and even the development of ankylosis. Conclusion: Treatment plan of condylar fracture should be based on the type of the fracture. Open reduction and rigid internal fixation were effective in the treatment of condylar fractures, however, surgical approach and osteosynthesis should be carefully chosen as the complications were correlated with these two factors.
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    Synergistic Anti-tumor Activity of Scutellarein and ABT-737 on Head and Neck Squamous Cell Carcinoma
    JING Guang-ping, LV Ke-wen, ZHANG Han, LV Xing
    2016, 32(11): 1188-1192.  DOI: 10.13701/j.cnki.kqyxyj.2016.11.019
    Abstract ( 211 )   PDF (1204KB) ( 252 )  
    Objective: To investigate the synergistic anti-tumor activity of Scutellarein and ABT-737 on head and neck squamous cell carcinoma HN30 cell line in vitro and to discuss the mechanisms. Methods: Inhibitory effects of ABT-737 and Scutellarein on HN30 cells were detected by MTT assay. Cell apoptosis was analyzed by flow cytometry. Mitochondrial transmembrane potential (ΔΨm) was analyzed by JC-1 staining. The expression of the protein related to apoptosis was quantified by western blot. Genes silencing was conducted by SiRNA. Results: Scutellarein and ABT-737 could synergistically inhibit the proliferation of HN30 cells in vitro in a dose-dependent manner. Scutellarein and ABT-737 could synergistically induce the apoptosis of HN30 cells, induce the Mitochondrial membrane potential depolarization, down-regulate the expression of Bcl-2 and Bcl-XL, and up-regulate the expression Bax. Inhibition of the expression of Mcl-1 induced by SiRNA could significantly enhance the synergistic effect of scutellarein and ABT-737 on HN30 cells apoptosis. Conclusion: Scutellarein and ABT-737 could synergistically induce the apoptosis of HN30 cells in vitro. The expression of Mcl-1 was down regulated by scutellarein and was correlated with the drug resistance for ABT-737.
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    Effects of Midazolam on Children 's Anxiety and Cortisol Level
    SU Cen-ping, XU Hong-yu
    2016, 32(11): 1193-1195.  DOI: 10.13701/j.cnki.kqyxyj.2016.11.020
    Abstract ( 347 )   PDF (791KB) ( 292 )  
    Objective: To investigate the effects of midazolam on anxiety levels and salivary cortisol levels in children after intranasal and sublingual midazolam, and to explore the possibility of mild sedation in Department of Pediatric Stomatology. Methods: Eighty-two patients with 3-7 years old were included in this study and were randomly divided into two groups. Patients received intranasal or sublingual midazolam at the 30 minutes prior to oral treatment. The anxiety levels were assessed using Venham clinical anxiety scale per 5 minutes and salivary cortisol levels were assessed 30 minutes before and after administration. Results: There was a significant decrease in the anxiety level from T0 to T1-T4 in group A and group B (P<0.05). There was no significant difference between two groups in the levels of salivary cortisol before and after taking the medicine. Conclusion: Midazolam can effectively relieve anxiety in children patients and has no effect on salivary cortisol levels. The tongue way was better absorption than the nasal route.
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    Accuracy of CAD/CAM Template for Implant Site Preparation
    WANG Jin-sheng, XU Liang-wei, JIANG Chen-bo, XU Ban, SHAO Li-min, ZHANG Fei-fei
    2016, 32(11): 1196-1199.  DOI: 10.13701/j.cnki.kqyxyj.2016.11.021
    Abstract ( 219 )   PDF (864KB) ( 384 )  
    Objective: To evaluate the accuracy of the CAD/CAM implant template based on CBCT data for dental implants preparation. Methods: Jaws from 6 patients were scanned using cone beam computed tomography (CBCT). The CT data was used to fabricated CAD/CAM implant templates. Twenty-four implants were inserted in edentulous patients using CAD/CAM implant templates. All patients had CBCT scanning again after operation. Preoperative and postoperative computed tomographic images were compared, and the deviations between actual and planned implants were measured. Results: With the help of CAD/CAM implant templates, the neck deviation of the implant was 1.03±0.55mm, apical deviation was 1.19±0.56mm, and axis deviation was 3.12±2.64°. Conclusion: The CAD/CAM implant template can accurately transfer the preoperative design, reduce the risk of operation, and is worth for clinical use.
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    Detection and Negotiation the Middle Mesial Canals in Mandibular Molars by Microscope
    YANG Li-li, ZHANG Yan, ZHAO Shi-jun, HU Shu-e, XU Jie, Xu Zhi-yuan
    2016, 32(11): 1200-1203.  DOI: 10.13701/j.cnki.kqyxyj.2016.11.022
    Abstract ( 238 )   PDF (872KB) ( 367 )  
    Objective: To evaluate the clinical effect of using dental operating microscope to detect and negotiate the middle mesial canals in mandibular molars. Methods: One hundred and thirty-nine mandibular molars from 126 patients were recruited. The detection and negotiation of middle mesial canals in mandibular molars were performed by three senior endodontists by naked eye or with the aid of dental operating microscope. The location of middle mesial canal orifices was recorded and the pathway of the canals was characterized by periapical angulated projection of X ray. Results: With the aid of microscope, the total number of detected and negotiated middle mesial canals increased by 11.5% and 10.1% respectively compared with naked eye. The number of negotiated middle mesial canals increased from 5.0% to15.0% in the first molars, and from 3.4% to 13.6% in the second molars. The number of detected middle mesial canals in the first and second molars increased by 12.5% and 10.2% under microscope. All of the detected middle mesial canal orifices were located in the mesial subpulpal groove, closer to the mesiolingual canal (46.2%), in the middle of the groove (30.8%), or closer to the mesiobuccal canal (23.1%). There was 45% of the negotiated confluenting with the mesiolingual canal and there was no middle mesial canals with an independent apical foramen. Conclusion: With the aid of dental operating microscope, the percentages of detected and negotiated middle mesial canals in mandibular molars were increased significantly.
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    Evaluation of Butorphanol Combined with Tetracaine Hydrochloride Jelly for the Prevention of Catheter-related Bladder Discomfort
    WANG Li, PENG Wei, ZHANG Tie-jun, LIU Ke-bin, HUANG Li-li, GONG Ping
    2016, 32(11): 1204-1207.  DOI: 10.13701/j.cnki.kqyxyj.2016.11.023
    Abstract ( 237 )   PDF (795KB) ( 271 )  
    Objective: To investigate the effects of butorphanol combined with Tetracaine Hydrochloride Jelly on catheter-related bladder discomfort. Methods: Ninety male patients undergoing selective maxillofacial plastic surgery were selected and divided into three groups. In group A, paraffin oil was used to daub the front end of catheter. In group B and group C, Tetracaine Hydrochloride Jelly was used to daub the front end of catheter. Meanwhile, in group C, butorphanol (20 μg/kg) was intravenously injected ten minutes before the end of operation. MAP, HR and SpO2 were recorded at 0, 1, 2, 3, 6 hours after tracheal estuation. Agitation and urinary tract irritation in awakening period of patients were also recorded. Results: The incidence and scores of CRBD at 0h and 1h after tracheal extubation were significantly lower in group B than group A (P<0.05), and those indexes in group C was the lowest (P<0.05). Conclusion: Combination of butorphanol and Tetracaine Hydrochloride Jelly injected into the urethral of male patients during general anesthesia can reduce the CRBD at tracheal extubation immediately and 1 hour after tracheal extubation.
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    Clinical Study of Ozone in Treatment of Temporomandibular Arthritis
    DENG Li, ZHANG Qing-Bin, ZHANG Ying, HE Xia
    2016, 32(11): 1208-1210.  DOI: 10.13701/j.cnki.kqyxyj.2016.11.024
    Abstract ( 227 )   PDF (773KB) ( 313 )  
    Objective: To investigate the clinical effect of injection of ozone into temporomandibular joint cavity in the treatment of temporomandibular arthritis. Methods: Sixty patients diagnosed as temporomandibular arthritis in our hospital were included from June 2014 to June 2015. The patients were randomly divided into two groups, 30 patients in the treatment group and 30 patients in the control group. Patients in the treatment group were treated with injection of ozone into the temporomandibular joint cavity while patients in the control group were treated with the injection of triamcinolone acetonide into the temporomandibular joint cavity. The curative effects in the two groups were compared. Results: The VAS score and maximum mouth opening showed insignificant difference between two groups. Conclusion: The injection of ozone into the temporomandibular joint cavity is clinically as effective as that of triamcinolone acetonide, meanwhile it showed fewer complications and higher security.
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    Primary Gingival B Cell Lymphoma: 1 Case Report
    CUI Qing-yin, FU Shuai, ZHANG Chang-bin, MA Kai-yu, LI Ming
    2016, 32(11): 1211-1212.  DOI: 10.13701/j.cnki.kqyxyj.2016.11.025
    Abstract ( 249 )   PDF (867KB) ( 245 )  
    A cases of primary gingival B cell lymphoma is reported in this paper. The patientwas male, 28-years-old with the left maxillary gingival painless gradually proliferated for 1 month. Special examination showed a 2cm×1.5cm×1cm tumor located in 23, 24 and 25 of the buccal gingiva. Image tests revealed a broadened periodontal membrane. The buccal bone was low density image with not clear boundary. Histopathologically,abnormallymphocyteswere seen arranged in disperse. Immunohistochemicalexamination demonstrated: CD20(-),CD2(-),CD10(+),CD138(+),CD79a(+),LCA(+), and Ki-67 around 80%(+).The diagnosis was primary gingival B cell lymphoma.
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    Treat-to-target in Oral Lichen Planus: Perspective and Prospective
    LU Rui
    2016, 32(11): 1213-1216.  DOI: 10.13701/j.cnki.kqyxyj.2016.11.026
    Abstract ( 758 )   PDF (1148KB) ( 425 )  
    Hitherto, oral lichen planus (OLP) is still an uncurable chronic disease. How to control the disease relapse and maintain long-term remission have not been well resolved. Treat-to-target is a novel treatment concept for the chronic diseases, which highlights treatment toward a therapeutic target of clinical remission by regular assessing disease activity and adjusting therapy accordingly. In recent years, this strategy has been widely applied in many areas of medicine and markedly improves the patients' condition and outcome. According to the concept of treat-to-target and current situation of OLP treatment, this article proposes an treat-to-target strategy in OLP and discusses its feasibility and key issues, aiming to provide an advisory opinion for the improvement of OLP treatment mode.
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    Important Role of Periodontal Biotype in Orthodontic Treatment
    ZHANG Rui, XUE Fei,HUANG Xiao-feng
    2016, 32(11): 1217-1220.  DOI: 10.13701/j.cnki.kqyxyj.2016.11.027
    Abstract ( 366 )   PDF (829KB) ( 624 )  
    The morphology of periodontal tissues varies from person to person. Forms of periodontal tissues have different responses to the related oral treatments. The concept of periodontal biotype has important significance for the clinical effect, prognosis and aesthetics. This paper will briefly outline the classification and influencing factors of periodontal biotype, and discuss the important role it plays on the clinical application of orthodontic treatments.
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