Synergistic Anti-tumor Activity of Scutellarein and ABT-737 on Head and Neck Squamous Cell Carcinoma

doi:10.13701/j.cnki.kqyxyj.2016.11.019

Abstract

Abstract: Objective: To investigate the synergistic anti-tumor activity of Scutellarein and ABT-737 on head and neck squamous cell carcinoma HN30 cell line in vitro and to discuss the mechanisms. Methods: Inhibitory effects of ABT-737 and Scutellarein on HN30 cells were detected by MTT assay. Cell apoptosis was analyzed by flow cytometry. Mitochondrial transmembrane potential (ΔΨm) was analyzed by JC-1 staining. The expression of the protein related to apoptosis was quantified by western blot. Genes silencing was conducted by SiRNA. Results: Scutellarein and ABT-737 could synergistically inhibit the proliferation of HN30 cells in vitro in a dose-dependent manner. Scutellarein and ABT-737 could synergistically induce the apoptosis of HN30 cells, induce the Mitochondrial membrane potential depolarization, down-regulate the expression of Bcl-2 and Bcl-XL, and up-regulate the expression Bax. Inhibition of the expression of Mcl-1 induced by SiRNA could significantly enhance the synergistic effect of scutellarein and ABT-737 on HN30 cells apoptosis. Conclusion: Scutellarein and ABT-737 could synergistically induce the apoptosis of HN30 cells in vitro. The expression of Mcl-1 was down regulated by scutellarein and was correlated with the drug resistance for ABT-737.

Key words: Scutellarein, ABT-737, Head and neck squamous cell carcinoma, Synergistic

CLC Number:

R739.8

JING Guang-ping, LV Ke-wen, ZHANG Han, LV Xing. Synergistic Anti-tumor Activity of Scutellarein and ABT-737 on Head and Neck Squamous Cell Carcinoma[J]. Journal of Oral Science Research, 2016, 32(11): 1188-1192.

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