Effect and Mechanism of Enamel Matrix Protein Derivatives on Osteogenic Differentiation of Periodontal Ligament Stem Cells Infected with Porphyromonas Gingivalis

doi:10.13701/j.cnki.kqyxyj.2020.06.008

Abstract

Abstract: Objective: To investigate the effect and mechanism of enamel matrix protein derivatives on osteogenic differentiation of porphyromonas gingivalis-infected periodontal ligament stem cells (PDLSCs). Methods: PDLSCs were digested and passaged after primary culture. The cells of the 3rd-4th generation were divided into groups. The control group was treated routinely and osteoblast differentiated. The infected group was osteoblast differentiated after 24 hours of porphyromonas gingivalis infection. The enamel matrix protein derivative group was osteoblast differentiated after 24 hours of porphyromonas gingivalis infection and added with 25 mg/L, 50 mg/L, and 100 mg/L enamel matrix protein derivatives during differentiation. 100 mg/L enamel matrix protein derivatives+TNF-α group was osteoblast differentiated after 24 hours of porphyromonas gingivalis infection and added with 100 mg/L enamel matrix protein and 10 ng/mL TNF-α derivatives during differentiation. The number of mineralized nodules, ALP activity, and expression of osteogenic marker genes Runx2, OCN, COL-I and signal pathway molecules p65 NF-κB, p-I κB and I κB were detected. Results: The number of mineralized nodules, ALP activity, and the expression of Runx2, OCN, COL-I, and I-κB in 25 mg/L, 50 mg/L and 100 mg/L enamel matrix protein derivatives groups were significantly higher than those in the infected group, while the expression of p65 NF-κB and p-I κB were significantly lower than those in the infected group. The number of mineralized nodules, ALP activity, and the expression of Runx2, OCN, COL-I and I-κB in 100 mg/L enamel matrix protein derivatives+TNF-α group were significantly lower than those in the 100 mg/L enamel matrix protein derivatives group, while the expression of p65 NF-κB and p-I κB were significantly higher than those in the 100 mg/L enamel matrix protein derivatives group. Conclusion: Enamel matrix protein derivatives can promote the osteogenic differentiation of porphyromonas gingivalis infected PDLSCs by inhibiting the NF-κB pathway.

Key words: periodontal ligament stem cells, porphyromonas gingivalis, enamel matrix protein derivatives, osteogenic differentiation

WU Yangxuan, XIANG Li, HUANG Zhiqiang, CHU Jinhai. Effect and Mechanism of Enamel Matrix Protein Derivatives on Osteogenic Differentiation of Periodontal Ligament Stem Cells Infected with Porphyromonas Gingivalis[J]. Journal of Oral Science Research, 2020, 36(6): 534-538.

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