Effect of SDF-1 on CXCR4 Expression of Human Periodontal Stem Cell Chemokine Receptor

doi:10.13701/j.cnki.kqyxyj.2020.01.013

Abstract

Abstract: Objective: To confirm the effect of human periodontal ligament stem cells (hPDLSCs) on expression of chemokine receptor CXCR4, and the effect of chemokine SDF-1 and blocker AMD3100 on expression of CXCR4. Methods: hPDLSCs were isolated and purified by digestible tissue block method combined with limited dilution method. The third generation of hPDLSCs were randomly divided into three groups: negative control group, SDF-1 group (200μg/L SDF-1), and SDF-1+AMD3100 group (200μg/L SDF-1+10 mg/L AMD3100). Western blotting was used to detect the expression of CXCR4 on hPDLSCs and the expression of CXCR4 under the action of SDF-1 and AMD3100. Results: The selected hPDLSCs could be induced to differentiate into osteoblasts and adipocytes. The cell phenotype was detected by flow cytometry to identify the immunophenotype consistent with periodontal ligament stem cells. Western blotting analysis showed that CXCR4 was expressed on hPDLSCs, and the expression of CXCR4 was up-regulated after SDF-1. There was no significant change in SDF-1+AMD3100 group. Conclusion: hPDLSCs could be obtained from the periodontal ligament of fresh isolated teeth and purified as a mesenchymal source after purification by limiting dilution method. The CXCR4 receptor was expressed on hPDLSCs. SDF-1 up-regulated CXCR4 receptor on hPDLSCs, and AMD3100 blocked the binding of SDF-1 to CXCR4 receptor.

Key words: SDF-1, CXCR4, human periodontal ligament stem cells, orthodontic tooth movement, AMD3100

LIU Pingping, TANG Xiaoying, YUAN Xiaoping. Effect of SDF-1 on CXCR4 Expression of Human Periodontal Stem Cell Chemokine Receptor[J]. Journal of Oral Science Research, 2020, 36(1): 51-55.

References

[1] 张华菁,李盛楠,徐晓南.力与炎症因子对牙周膜细胞破骨调控因子表达的影响[J].中国医学科学院学报,2017,39(5):602-610.
[2] Lim HC, Cha BY, Song SU, et al. Harmine promotes periodontal ligament cell-induced tissue regeneration [J]. Oral Dis, 2018, 24(3):456-464.
[3] Chen Z, Liu HL. Restoration of miR-1305 relieves the inhibitory effect of nicotine on periodontal ligament-derived stem cell proliferation, migration, and osteogenic differentiation [J]. J Oral Pathol Med, 2017, 46(4):313-320.
[4] Chen L, Mo S, Hua Y. Compressive force-induced autophagy in periodontal ligament cells downregulates osteoclastogenesis during tooth movement[J]. J Periodontol, 2019, 90(10):1170-1181.
[5] 唐伟,刘建国,梁文红,等.茶多酚对脂多糖下牙周膜细胞作用的研究进展[J].临床口腔医学杂志,2018,34(3):180-183.
[6] Yu Y, Mu J, Fan Z, et al. Insulin-like growth factor 1 enhances the proliferation and osteogenic differentiation of human periodontal ligament stem cells via ERK and JNK MAPK pathways [J]. Histochem Cell Biol, 2012, 137(4): 513-525.
[7] 路红艳,李健,龙星.间充质干细胞表面标记在颞下颌关节疾病患者滑膜间充质细胞表达的比较[J].口腔医学研究,2018,34(11):1249-1252.
[8] 刘悦明,钟良军. 牙周膜干细胞的研究进展[J].临床口腔医学杂志,2010 26(9): 570-572.
[9] 姜杨杨,姜竹玲,刘明月,等.牙源性干细胞及其在再生医学中的应用进展[J].医学综述,2018,24(12):2334-2338.
[10] 周静. 牙髓、牙周组织中骨髓来源细胞特性的实验研究[D].第四军医大学,2010.
[11] Bae YK, Kim GH, Lee JC, et al. The significance of SDF-1α-CXCR4 axis in in vivo angiogenic ability of human periodontal ligament stem cells [J]. Mol Cells, 2017, 40(6):386-392.
[12] 周瑞明,曾荣.SDF-1/CXCR4拮抗剂AMD3100应用方面的研究进展[J].广东医学院学报,2010,28(2):205-207.
[13] Du L, Yang P, Ge S. Stromal cell-derived factor-1 significantly induces proliferation, migration, and collagen type I expression in a human periodontal ligament stem cell subpopulation [J]. J Periodontol, 2012, 83(3): 379-388.
[14] 张劼,项杰,秦映芬. SDF-1/CXCR4轴促进慢性创伤愈合作用机制的研究进展[J]. 中国临床医学, 2017,10(6):596-599.
[15] 张罡瑜,杭秋琦.趋化因子SDF-1 研究进展[J].中国民族民间医药,2016 ,25(20):51-55.