Expression of Chemokine Receptor CXCR7 in Periodontitis and Its Mechanism

doi:10.13701/j.cnki.kqyxyj.2020.01.012

Abstract

Abstract: Objective: To investigate the expression and distribution of CXCR7 in periodontitis and the role of SDF-1/CXCR7 biological axis in periodontitis. Methods: Immunohistochemical method and PCR were used to detect the expression of SDF-1, CXCR4, and CXCR7 in 15 normal gingival tissues and 15 periodontitis tissues. Results: The expressions of SDF-1, CXCR4, and CXCR7 in periodontitis tissues were higher than those in normal gingival tissues (P<0.05). There was a positive correlation between SDF-1 and CXCR7 (γ=0.533, P=0.041), and between CXCR4 and CXCR7 (γ=0.533, P=0.033,P<0.01). CXCR7 was expressed in inflammatory cells of periodontitis and vascular endothelial cells associated with inflammation. Conclusion: The overexpression of CXCR7 in the microenvironment of periodontitis may play a role in the pathological process of periodontitis, which may be a new target for the treatment of periodontitis.

Key words: SDF-1, CXCR4, CXCR7, periodontitis

ZHANG Yan, XIN Qi, MA Zhe, WEI Rong, LI Xiaodong, SU Rui, ZHANG Yuan. Expression of Chemokine Receptor CXCR7 in Periodontitis and Its Mechanism[J]. Journal of Oral Science Research, 2020, 36(1): 46-50.

References

[1] 范华南,黄辉,姜庆坤,等.中度和重度牙周炎患者龈下细菌多样性和群落结构分析[J].口腔医学研究,2018,34(8):846-851.
[2] Zlotnik A, Yoshie O. The chemokine superfamily revisited [J]. Immunity, 2012, 36(5):705-716.
[3] Gojska-Grymajlo A, Zielinski M, Wardowska A, et al. CXCR7⁺ and CXCR4⁺ stem cells and neuron specific enolase in acute ischemic stroke patients [J]. Neurochem Int, 2018, 120:134-139.
[4] Hosokawa Y, Hosokawa I, Ozaki K, et al. CXCL12 and CXCR4 expression by human gingival fibroblasts in periodontal disease [J]. Clin Exp Immunol, 2005, 141(3):467-474.
[5] Sun J, Nemoto E, Hong G, et al. Modulation of stromal cell-derived factor 1 alpha (SDF-1alpha) and its receptor CXCR4 in Porphyromonas gingivalis-induced periodontal inflammation [J]. BMC Oral Health, 2016, 17(1):26.
[6] Grdovic N, Rajic J, Petrovic SM, et al. Association of CXCL12 gene promoter methylation with periodontitis in patients with diabetes mellitus type 2 [J]. Arch Oral Biol, 2016, 72:124-133.
[7] Gu HQ, Zhang ZB, Zhang JW, et al. The role of the SDF-1/ CXCR7 axis on the growth and invasion ability of endometrial cancer cells [J]. Arch Gynecol Obstet, 2017, 295(4):987-995.
[8] Guan S, Zhou J. CXCR7 attenuates the TGF-beta-induced endothelial-to-mesenchymal transition and pulmonary fibrosis [J]. Mol Biosyst, 2017, 13(10):2116-2124.
[9] Kallifatidis G, Munoz D, Singh RK, et al. beta-Arrestin-2 counters CXCR7-mediated EGFR transactivation and proliferation [J]. Mol Cancer Res, 2016, 14(5):493-503.
[10] Pluchino N, Mamillapalli R, Moridi I, et al. G-protein-coupled receptor CXCR7 is overexpressed in human and murine endometriosis [J]. Reprod Sci, 2018, 25(8):1168-1174.
[11] Yashiro Y, Nomura Y, Kanazashi M, et al. Function of chemokine (CXC motif) ligand 12 in periodontal ligament fibroblasts [J]. PLoS One, 2014, 9(5):e95676.
[12] Kaku M, Kitami M, Rosales Rocabado JM, et al. Recruitment of bone marrow-derived cells to the periodontal ligament via the stromal cell-derived factor-1/C-X-C chemokine receptor type 4 axis [J]. J Periodontal Res, 2017, 52(4):686-694.
[13] Zhang M, Qiu L, Zhang Y, et al. CXCL12 enhances angiogenesis through CXCR7 activation in human umbilical vein endothelial cells [J]. Sci Rep, 2017, 7(1):8289.
[14] Werner TA, Forster CM, Dizdar L, et al. CXCR4/CXCR7/CXCL12 axis promotes an invasive phenotype in medullary thyroid carcinoma [J]. Br J Cancer, 2017, 117(12):1837-1845.
[15] Yuan CL, Liu ZH, Zou N, et al. Relationship between expression of CXCR7 and NF-kappaB in breast cancer tissue and occurrence of breast cancer and lymphatic metastasis [J]. Saudi J Biol Sci, 2017, 24(8):1767-1770.
[16] Zhou Y, Cao HB, Li WJ, et al. The CXCL12 (SDF-1)/CXCR4 chemokine axis: Oncogenic properties, molecular targeting, and synthetic and natural product CXCR4 inhibitors for cancer therapy [J]. Chin J Nat Med, 2018, 16(11):801-810.
[17] Ando N, Furuichi Y, Kasai S, et al. Chemosensitivity is differentially regulated by the SDF-1/CXCR4 and SDF-1/CXCR7 axes in acute lymphoblastic leukemia with MLL gene rearrangements [J]. Leuk Res, 2018, 75:36-44.
[18] Song ZY, Wang F, Cui SX, et al. CXCR7/CXCR4 heterodimer-induced histone demethylation: a new mechanism of colorectal tumorigenesis [J]. Oncogene, 2019, 38(9):1560-1575.