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28 January 2020, Volume 36 Issue 1 Previous Issue    Next Issue

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Dental Caries Revisited. CHEN Zhi, CHEN Ruitian. 2020, 36(1): 1-6.  DOI: 10.13701/j.cnki.kqyxyj.2020.01.001 Abstract ( 742 )   PDF (996KB) ( 931 )   With the constant renewal and development of dental caries, the decision making of dental caries treatment has changed. Based on caries risk assessment of patients, by controlling the risk factors of dental caries and implementing non-invasive, minimally invasive and invasive caries prevention and treatment technology, making a comprehensive and personalized caries management has become a trend in modern oral health. References | Related Articles | Metrics

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Pathogenic Mechanism and Clearance Strategy of Bacterial Endotoxin in Infected Root Canal HOU Yajing, HUANG Dingming 2020, 36(1): 7-9.  DOI: 10.13701/j.cnki.kqyxyj.2020.01.002 Abstract ( 551 )   PDF (751KB) ( 594 )   Bacterial endotoxin plays an important role in the development of pulpitis and periapical periodontitis. The exploration of the pathogenesis of endotoxin and the elimination of endotoxin from the root canal system could contribute the success of root canal treatment and the long-term postoperative effect. In this review, the research progress on the pathogenic mechanism and the removing of bacterial endotoxin in pulpitis and periapical periodontitis was mainly introduced. References | Related Articles | Metrics

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Application Progress of Fiber Grating Sensing Technology in Oral Research ZHAO Zhen 2020, 36(1): 10-12.  DOI: 10.13701/j.cnki.kqyxyj.2020.01.003 Abstract ( 394 )   PDF (796KB) ( 372 )   Fiber Bragg Grating sensing technology has the advantages of small size, high sensitivity, good biocompatibility, and flexibility. Its non-radioactive and non-invasive measurement methods may change the way of oral research, and can be used as a substitute for traditional oral research and detection methods. This technology has attracted the attention of dentists and scholars in recent years. This paper reviews the fiber grating sensing method in the field of stomatology, including oral biomechanics, oral materials, oral laser therapy, and other research areas, in order to provide references for its further application References | Related Articles | Metrics

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Application and Progress of Murine in the Study of Osteogenesis Induced by Tissue Engineered Bone. WU Jia, LIU Zhihui 2020, 36(1): 13-15.  DOI: 10.13701/j.cnki.kqyxyj.2020.01.004 Abstract ( 352 )   PDF (750KB) ( 244 )   As a modern and excellent way, tissue engineered bone has been a research focus to repaire bone defects in recent decades. Because of its low cost, stable effect, and stimulation of human diseases, murine has become the widely used animal models for bone osteogenesis tests. In this paper, several factors including the species, sex, age, and immunity of murine, the transplantion methods of tissue engineered bone and the cites of bone defects are summarized based on the review of the relevant literature about bone osteogenesis experiments using tissue engineered bone within murine in recent years to provide references for the future researchers. References | Related Articles | Metrics

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Research Status of Cysteine Cathepsinsin Dentine. WEI Yu, WANG Jun 2020, 36(1): 16-19.  DOI: 10.13701/j.cnki.kqyxyj.2020.01.005 Abstract ( 288 )   PDF (776KB) ( 245 )   The degradation of dentin matrix collagen is an important factor leading to the destruction of the hybrid layer. Studies have confirmed that the degradation of dentin collagen is closely related to endogenous proteases, such as matrix metalloproteinases (MMPs). In 2010, cysteine cathepsins(CCs, CTs)were firstly detected in dentine, which could degrade collagen fibers. There are more and more studies on cysteine cathepsins at present, this paper will review the research progress on cysteine cathepsins in dentin. References | Related Articles | Metrics

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Effects of Gelatin Microspheres Sustained Release CGRP and SP on Osteogensis of Tooth Socket of Mice. SONG Zijian, DING Yuxiang, ZHOU Qin 2020, 36(1): 20-23.  DOI: 10.13701/j.cnki.kqyxyj.2020.01.006 Abstract ( 250 )   PDF (2446KB) ( 326 )   Objective: To explore whether the gelatin microspheres sustained release CGRP and SP can promote bone formation in tooth socket of mice. Methods: The gelatin microspheres containing 10-6 mol/L SP and CGRP were prepared and filled into the sockets of maxillary first molar on one side of 27 adult KM mice. The mice were divided into three groups, including SP, CGRP, and SP/CGRP mix groups randomly. 4 weeks later, the mice were sacrificed and the maxillae bone was harvested. The samples were embedded in methylmethacrylate for histological morphology examination. Results: The average BV/TV values were (30.25±0.92)% in experimental socket and (10.66±0.83)% in control socket (P＜0.05)in SP group;(19.46±1.46)% in experimental socket and (8.78±1.03)% in control socket(P＜0.05)in CGRP group:(59.45±3.07)% in experimental socket and (10.06±3.25)% in control socket(P＜0.05)in SP/CGRP mix group. Bone volume/tissue volume (BV/TV) was (59.453±3.071)% in the mixed experimental group and (10.062±3.254)% in the mixed blank group (P<0.05). Trabecular thicknesses (Tb.Th) were (0.076±0.009) % in the mixed blank group and 0.132±0.043 % in the mixed treatment group (P<0.05). Trabecular number (Tb.N) were (0.841±0.212)% in the mixed blank group and (4.497±0.413)% in the mixed treatment group (P<0.05). Bone mineral density (BMD) was (0.184±0.072)% in mixed blank group was and (0.615± 0.010)% in mixed experimental group (P<0.05). Conclusion: CGRP and SP could promote osteogenesis individually and promote osteogenesis synergistically. References | Related Articles | Metrics

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Clinical Study on Nasal Airflow in Patients with Unilateral Complete Cleft Lip and Palate SONG Juan 2020, 36(1): 24-27.  DOI: 10.13701/j.cnki.kqyxyj.2020.01.007 Abstract ( 241 )   PDF (1217KB) ( 190 )   Objective: To study the correlation between nasal airflow and internal nasal malformation in patients with unilateral complete cleft lip and palate. Methods: 49 patients with unilateral cleft lip and palate at the age of 9-11 years old were selected as the study group. 32 children with noncleft at the corresponding age were selected as the control group. The nasal resistance tester was used to measure the ventilation. The resistance was correlated with the corresponding measurement data of the internal nasal malformation. Results: Of the 49 patients, the intranasal malformation was measured by nasal septum deviation. The distance of deviation of the nasal septum was 9.12±3.76mm, and the angle of deviation was (20.8±2.7)°. The distance of the nasal septum deviation and the deviation angle of the patients with unilateral cleft lip and palate were larger than those of the control group (P<0.05). The mean value of nasal resistance values in the cleft lip and palate group was (0.832±0.254) Pa/(s·cm3), and the mean nasal resistance of the control group was (0.627±0.167) Pa/(s·cm3), (P<0.05). The cleft lip and palate group had a larger nasal resistance value than the control group. The size of nasal resistance was positively correlated with the distance between the nasal septum and the angle of deflection (r=0.754, P<0.001, r=0.652, P<0.001). Conclusion: Intranasal malformation in patients with unilateral complete cleft lip and palate was severe, which might lead to increased nasal ventilation resistance. References | Related Articles | Metrics

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Effect of β2-adrenergic Signal Transduction on Subchondral Bone Loss of Rat Mandibular Condyles Induced by Abnormal Molar Occlusion. LIU Geng, LI Le, XING Lu, FAN Xiangfei 2020, 36(1): 28-32.  DOI: 10.13701/j.cnki.kqyxyj.2020.01.008 Abstract ( 215 )   PDF (3224KB) ( 161 )   Objective: To investigate the effects of β2-adrenergic signal transduction on the subchondral bone loss of rat mandibular condyles induced by abnormal molar occlusion. Methods: Abnormal molar occlusion (experimental group) and their sham control (control group) were established on rats by orthodontic methods. The experimental and control rats were intraperitoneally injected by normal saline (vehicle injection group) or β-adrenergic receptor blocker propranolol (propranolol injection group), respectively. Micro-CT was used to detect the changes of bone mineral density and microstructures of mandibular condylar subchondral bone in each group. The concentration of norepinephrine in subchondral bone was determined by enzyme-linked immunosorbent assay, while the gene expressions of adrenergic receptors were detected by real-time quantitative polymerase chain reaction and immunohistochemistry. Results: Bone mineral density (BMN), bone volume fraction (BVF), and trabecular thickness (Tb.Th) of the condylar subchondral bone decreased, while trabecular space (Tb.Sp) and norepinephrine content increased in 4- and 8-week experimental groups than those of the age-matched controls, respectively (P<0.05). The gene and protein expression of Adrb2 in the subchondral bone of the experimental group was significantly higher than those of the controls (P<0.05), while the gene expression of Adrb1 and Adrb3 were not significantly different between control and experimental groups (P>0.05). Propranolol injection significantly reversed the loss of condylar subchondral bone induced by abnormal occlusion, and the parameters of BMD and trabecular microstructures of condylar subchondral bone were significantly improved compared with the vehicle injection group (P<0.05). Conclusion: Activation of β2-adrenergic signal transduction promotes the subchondral bone loss of rat mandibular condyles induced by abnormal molar occlusion. References | Related Articles | Metrics

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Effect of CBCT on Selection of Extraction Method for Mandibular Third Molar WANG Xuetao, LI Cong, LI Xiangchun, LI Fangning 2020, 36(1): 33-36.  DOI: 10.13701/j.cnki.kqyxyj.2020.01.009 Abstract ( 331 )   PDF (1746KB) ( 261 )   Objective: To evaluate the effect of CBCT on the selection of extraction method for mandibular third molar. Methods: 130 cases of mandibular third molar were selected, and two radiographic methods were used for preoperative examination.(1) Panoramic radiograph examination ; (2) CBCT examination. After radiological examination, the surgical procedure was determined: surgical removal or coronectomy. The first treatment plan was formulated according to the panoramic image, and the second was determined according to CBCT. After the CBCT was taken, the factors for the selection of coronectomy were recorded. Results: The operative plan was performed according to the second-step treatment plan. The first treatment plan of 113 (86.9%) samples was consistent with the second treatment plan, while the treatment plan of 17 (13.1%) samples after CBCT was changed. When the mandibular third molar was in direct contact with the inferior alveolar neural tube, the final choose was to use the coronectomy. However, direct contact was not an adequate influencing factor. In addition, the number of roots, the presence of cortical bone between the roots and the neural tube, the location of the neural tube in the hollow of the root, and the length of the contact between the root and the neural tube were also important factors influencing the selection of the coronal amputation. Conclusion: CBCT affected 13.1% of treatment plans. The direct contact between mandibular third molars and neural tube cavities, the position of the neural tube bending or depression near the apical complex, the presence of cortical bone between the root and neural tube, and the length of the root and neural tube contact observed in CBCT images were important factors influencing the decision of coronal amputation. References | Related Articles | Metrics

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Effects of Estrogen and Progesterone Sustained Release Gel on Alveolar Bone Regeneration of Ovariectomized Periodontitis Rats YUAN Gongjie, DU Xinjie, SHEN Lanhua 2020, 36(1): 37-40.  DOI: 10.13701/j.cnki.kqyxyj.2020.01.010 Abstract ( 269 )   PDF (3497KB) ( 172 )   Objective: To study the effect of estrogen-progesterone sustained-release gel on the regeneration of alveolar bone in OVX periodontitis rats. Methods: Female rats aged 6 months were randomly divided into two groups: the ovariectomized group (extracted rat bilateral ovaries) and the sham operation group (bilateral ovarian adipose tissue was removed by the same method). Serum calcium, phosphorus, alkaline phosphatase, and E2 concentrations were detected 12 weeks after ovariectomized. After 12 weeks, a 0.2 mm ligature was ligated to the unilateral first molar. After ligation for 4 weeks, a rat model of periodontitis was established and bone loss was observed by Micro-CT. After removing the ligation, the rats were divided into sham saline group, sham treatment group, OVX saline group, and OVX treatment group. Estrogen-progesterone sustained-release gel and saline were injected into the periodontal pocket of rats, respectively, and Micro-CT analysis and HE histological staining were performed after 8 weeks of treatment. Results: After ovariectomized, the bone mineral density and calcium, phosphorus, and E2 concentrations of serum were reduced, however, ALP activity was increased. After periodontal ligation, alveolar bone was absorbed in horizontal direction in sham group. However, alveolar bone was absorbed not only in horizontal but also in vertical direction in OVX group, and the root bifurcation was exposed. After application of estrogen and progesterone sustained-release gel, alveolar bone density (BMD) was increased, trabecular bone number (Tb.N) was increased, trabecular bone separation degree (TbTh) was increased, trabecular bone connection number (Tb.P) was increased, and alveolar bone formation was increased. Conclusion: Estrogen and progesterone sustained-release gel promotes the alveolar bone regeneration of OVX periodontitis rats. References | Related Articles | Metrics

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Effects of Platelet-rich Fibrin Extract on Periodontal Ligament Cells HUANG Yizhi, GUO Jun, YANG Jian 2020, 36(1): 41-45.  DOI: 10.13701/j.cnki.kqyxyj.2020.01.011 Abstract ( 224 )   PDF (3499KB) ( 175 )   Objective: To investigate the effect of platelet-rich fibrin extract (PRF) on the proliferation, migration, and osteogenesis of human periodontal ligament cells (hPDLCs). Methods: The hPDLCs were primarily cultured. After identification, the effect of PRF on the proliferation of hPDLCs was detected by MTT. The cells was divided into control group and PRF group (concentration 50%). The ALP activity of hPDLCs cultured for 7d and 14d was detected by alkaline phosphatase assay. The migration of hPDLCs was detected by transwell. The mineralization function of cells was observed by alizarin red staining. The expression of BMP2 and Runx2 was detected by western blotting. Results: MTT results showed that OD value of each group increased gradually with time, and the fastest increase was in PFR2 group. Compared with the control group, ALP activity in PRF group increased significantly on day 7 and 14 (P<0.05). Transwell results showed that the number of migrating cells in PFR group was significantly higher than that in control group (P<0.05). Alizarin red staining showed that the optical density of mineralized nodules in PRF group was higher than that in control group at all time points (P<0.05). Western blotting results showed that the expressions of Runx2 and BMP2 in PFR group were higher than those in control group (P<0.05). Conclusion: PRF can promote the proliferation, migration, and osteogenic differentiation of hPDLCs, and its mechanism of promoting bone formation needs further study References | Related Articles | Metrics

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Expression of Chemokine Receptor CXCR7 in Periodontitis and Its Mechanism ZHANG Yan, XIN Qi, MA Zhe, WEI Rong, LI Xiaodong, SU Rui, ZHANG Yuan 2020, 36(1): 46-50.  DOI: 10.13701/j.cnki.kqyxyj.2020.01.012 Abstract ( 202 )   PDF (1789KB) ( 132 )   Objective: To investigate the expression and distribution of CXCR7 in periodontitis and the role of SDF-1/CXCR7 biological axis in periodontitis. Methods: Immunohistochemical method and PCR were used to detect the expression of SDF-1, CXCR4, and CXCR7 in 15 normal gingival tissues and 15 periodontitis tissues. Results: The expressions of SDF-1, CXCR4, and CXCR7 in periodontitis tissues were higher than those in normal gingival tissues (P<0.05). There was a positive correlation between SDF-1 and CXCR7 (γ=0.533, P=0.041), and between CXCR4 and CXCR7 (γ=0.533, P=0.033,P<0.01). CXCR7 was expressed in inflammatory cells of periodontitis and vascular endothelial cells associated with inflammation. Conclusion: The overexpression of CXCR7 in the microenvironment of periodontitis may play a role in the pathological process of periodontitis, which may be a new target for the treatment of periodontitis. References | Related Articles | Metrics

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Effect of SDF-1 on CXCR4 Expression of Human Periodontal Stem Cell Chemokine Receptor LIU Pingping, TANG Xiaoying, YUAN Xiaoping 2020, 36(1): 51-55.  DOI: 10.13701/j.cnki.kqyxyj.2020.01.013 Abstract ( 210 )   PDF (4943KB) ( 195 )   Objective: To confirm the effect of human periodontal ligament stem cells (hPDLSCs) on expression of chemokine receptor CXCR4, and the effect of chemokine SDF-1 and blocker AMD3100 on expression of CXCR4. Methods: hPDLSCs were isolated and purified by digestible tissue block method combined with limited dilution method. The third generation of hPDLSCs were randomly divided into three groups: negative control group, SDF-1 group (200μg/L SDF-1), and SDF-1+AMD3100 group (200μg/L SDF-1+10 mg/L AMD3100). Western blotting was used to detect the expression of CXCR4 on hPDLSCs and the expression of CXCR4 under the action of SDF-1 and AMD3100. Results: The selected hPDLSCs could be induced to differentiate into osteoblasts and adipocytes. The cell phenotype was detected by flow cytometry to identify the immunophenotype consistent with periodontal ligament stem cells. Western blotting analysis showed that CXCR4 was expressed on hPDLSCs, and the expression of CXCR4 was up-regulated after SDF-1. There was no significant change in SDF-1+AMD3100 group. Conclusion: hPDLSCs could be obtained from the periodontal ligament of fresh isolated teeth and purified as a mesenchymal source after purification by limiting dilution method. The CXCR4 receptor was expressed on hPDLSCs. SDF-1 up-regulated CXCR4 receptor on hPDLSCs, and AMD3100 blocked the binding of SDF-1 to CXCR4 receptor. References | Related Articles | Metrics

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Clinical Evaluation of Collaborative Management in Treating Combined Periodontal-endodontic Lesions with Palatal Radicular Grooves. ZHANG Rui, ZHENG Dan, MENG Liuyan 2020, 36(1): 56-60.  DOI: 10.13701/j.cnki.kqyxyj.2020.01.014 Abstract ( 301 )   PDF (3314KB) ( 295 )   Objective: To evaluate the effect of combined endodontic microsurgery and periodontic management in treating palatal radicular groove. Methods: 11 teeth diagnosed as combined periodontal-endodontic lesion with palatal radicular grooves were collected. They were treated with root canal treatment under microscope and then combined endodontic microsurgery and periodontal management. Before surgery, 6 months, and one year follow-up after surgery, periodontal probing depth (PD), sulcus bleeding index(SBI), tooth mobility(TM) were measured. The data were statistically analyzed.The results of periapical lesion healing were evaluated by clinical and X-ray examination. Results: PD, SBI, and TM were significantly lowered 6 months and 1 year after surgery than before (P<0.05). The success rate at 6 months follow-up after surgery was 81.82%.The success rate at 1 year after operation was 90.91%. Conclusion: The combination of endodontic microsurgery and periodontal treatment is practical treatment modality in treating combined endodontic-periodontal lesions caused by palatal radicular groove. References | Related Articles | Metrics

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Effect of HtrA on Expression of gbpC of Streptococcus Mutans Isolated from Children with High Cario-susceptibility. HAN Linxiu, WANG Wang, WANG Ling, YANG Weihong,LIU Yao, LIU Xingrong 2020, 36(1): 61-64.  DOI: 10.13701/j.cnki.kqyxyj.2020.01.015 Abstract ( 196 )   PDF (1931KB) ( 190 )   Objective: To compare the expression of glucan-binding protein C (gbpC) of streptococcus mutans under normal outside environment between high temperature requirement serine proteinase A (HtrA) gene-deficient strains and high-virulent strains of streptococcus mutans (S.mutans) isolated from children with high cario-susceptibility. Methods: The HtrA-deficient strains and high virulent strains of S.mutaus were obtained by preliminary study. The strains were reanimated and incubated in BHI medium to exponential phase at 12th hour. The biochemical identification and Gram stain identification were used to identify them. The expression of gbpC gene and protein in S.mutans strains were detected by qPCR and Western Blot, respectively. Results: HtrA highly virulent strains had higher gbpC gene and protein expression levels than that of HtrA gene-deficient strains. Conclusion: The expression of gbpC in streptococcus mutans was affected by HtrA References | Related Articles | Metrics

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Influence of CSE on Remineralization of Early Enamel Caries WANG Jinfeng, CHE Jingyi, LIU Huimei, ZHU Xingguo, SUN Wenzhang, PAN Shuang 2020, 36(1): 65-69.  DOI: 10.13701/j.cnki.kqyxyj.2020.01.016 Abstract ( 204 )   PDF (2319KB) ( 156 )   Objective: To study the effects of ethanol extract of cucumis sativus seeds (CSE) on remineralization of early enamel caries. Methods: Bovine incisors were put into the demineralization solution (pH=5) to form the artifical enamel decay models. 52 bovine teeth with hardness of about 280 HV were selected and divided into 5 groups in randomly: 2, 10, 20 g/L ethanol extract of cucumis sativus seeds groups, 20 g/L NaF (positive control) group, and deionized water (negative control) group. Microhardness was tested and electron-probe was used to test the element of enamel surface before and after treating with different solutions. Results: After the remineralization treatment by three different concentrations of CSE and NaF, the enamel hardness in these groups was significantly increased than that before treatment (P<0.05), while the deionized water group showed no significant difference compared as before (P>0.05). Microhardness in different CSE groups was increased with the concentration of CSE increased, among which, the difference was statistically significant (P<0.05). Electron probe showed there was compact spherical homogeneous precipitation deposited on the surface of enamel in NaF group. While in deionized water group, the typical honeycomb structure was observed in the surface of enamel. In CSE gruops, irregular sediments were observed to deposit on the surface of decalcified enamel. The higher the concentration of CSE was, the more sediment was deposited on the surface. Conclusion: NaF had the best effect on early enamel recalculation. Suitable concentrations of ethanol extract of CSE have remineralization effects on artificial demineralized enamel. The best effect was obtained by 20 g/L ethanol extract of CSE, followed by 10 g/L and 2 g/L. References | Related Articles | Metrics

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Effects of Hypoxia on Autophagy and Myogenin Expression in Different Stages of Differentiation of C2C12 Cells. MAO Jiaqi, HUANG Wei, YU Liming, ZHAO Liru, YANG Dongru, LU Yun 2020, 36(1): 70-74.  DOI: 10.13701/j.cnki.kqyxyj.2020.01.017 Abstract ( 261 )   PDF (3292KB) ( 256 )   Objective: To study the effects of hypoxia on the expression of Beclin1, LC3B and myogenin in different stages of differentiation of C2C12 cells. Methods: C2C12 cells were induced to differentiate for 1, 2, 3 and 4 days. The differentiation of C2C12 cells was observed under normoxia and hypoxia. Immunocytochemistry was used to detect the expression of autophagy-related proteins LC3B and Beclin1. Western blotting was used to detect the protein expression levels of Beclin1, LC3B and myogenin. SPSS22.0 software package was used to analyze the data. Results: The number of myotubes in hypoxia group was less than that in normoxia group, and the morphology of cells was smaller. Immunocytochemistry showed that the fluorescence intensity of LC3B and Beclin1 of hypoxia group at 1, 2, 3 and 4 days of differentiation was lower than that in normoxia group. Western blotting showed that the expression levels of Beclin1, LC3B and myogenin in the early and middle stages of differentiation in hypoxic group were significantly lower than the normoxia group (P<0.05). With the increase of differentiation time, the expression level of LC3B decreased further, while the expression level of myogenin increased. Conclusions: Hypoxia inhibits the differentiation of C2C12 cells; hypoxia can reduce the expression levels of Beclin1, LC3B and myogenin in the early and middle stages of cell differentiation. References | Related Articles | Metrics

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Effect of Porphyromonas Gingivalis Lipopolysaccharide on Expression of Cav-1 in HUVECs ZUO Yuan,LI Weishan 2020, 36(1): 75-78.  DOI: 10.13701/j.cnki.kqyxyj.2020.01.018 Abstract ( 230 )   PDF (2310KB) ( 133 )   Objective: To study the effect of P. gingivalis lipopolysaccharide (P. g-LPS) on the expression of caveolin-1 (Cav-1) in human umbilical vein endothelial cells (HUVECs), and to explore the relationship between P. g-LPS and atherosclerosis (AS). Methods: HUVECs were cultured in vitro, and different concentrations of P.g-LPS (0.5, 1.0, 5.0, 10.0 mg/L) were selected to induce for 6, 18, 24 and 36 hours. The effects of P.g-LPS on the proliferation of HUVECs were detected by EdU method, the expression of Cav-1 in HUVECs was detected by ELISA, and the expression of Cav-1 was detected by RT-PCR. Results: After co-culture of P.g-LPS and HUVECs, the proliferation activity of HUVECs decreased compared with the control group. With the increase of P.g-LPS concentration, the proliferation activity of HUVECs decreased significantly (P<0.05). Compared with the control group, the expression of Cav-1 protein in HUVECs induced by P.g-LPS increased for 18 hours, sustained high expression for 24 and 36 hours, with the highest expression level at 36 hours (P<0.05); the expression of Cav-1 gene in HUVECs induced by P.g-LPS increased for 18 hours, and the expression of Cav-1 gene increased with the increase of concentration at 24 and 36 hours (P<0.001). Conclusion: P.g-LPS can significantly inhibit the proliferation activity of HUVECs and up-regulate the protein and molecular level of Cav-1 cells. It is suggested that P.g-LPS infection may cause endothelial cell disfunction, thereby accelerating the occurrence and development of AS References | Related Articles | Metrics

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Effects of Resveratrol on Proliferation and Differentiation of Osteoblasts Induced by High Glucose through SIRT1/FOXO1 Pathway TANG Xiaoyue, ZHU Hao, CAO Can 2020, 36(1): 79-84.  DOI: 10.13701/j.cnki.kqyxyj.2020.01.019 Abstract ( 264 )   PDF (2315KB) ( 182 )   Objective: To investigate the effects of resveratrol (Res) on the proliferation and differentiation of osteoblasts induced by high glucose and on the silence information regulator (SIRT1)/fork transcription factor (FOXO1) pathway. Methods: Rat mandibular cells were isolated and cultured, and the experiment was divided into control group, high-glucose group, high-glucose + low-dose Res group, high-glucose + medium-dose Res group, and high-glucose + high-dose Res group. CKK-8 assay was used to detect cell proliferation, ALP activity was determined by alkaline phosphatase (ALP) staining, Alizarin red S staining was used to detect the formation of calcified nodules and, Western blotting was used to detect the expressions of type I collagen (COLI) and RUNX2, osteopontin (OPN), osteocalcin (OCN), and SIRT1/FOXO1 pathway protein. Results: Compared with control group, inhibition in high-glucose group increased significantly, ALP activity, calcium nodule deposition, COLI, RUNX2, OPN, and OCN protein levels in high-glucose group decreased significantly (P<0.05). Compared with the high-glucose group, the OD value, ALP activity, calcium nodule deposition, COLI, RUNX2, OPN, and OCN protein levels of osteoblasts in resve- ratrol supplemented groups increased significantly (P<0.05), and increased with the increase of concentration. Compared with control group, the levels of SIRT1 and P27 protein of osteoblasts in high-glucose group decreased significantly, while the levels of FOXO1 and Bim increased significantly (P<0.05). Compared with the high-glucose group, the levels of SIRT1 and P27 protein increased significantly and FOXO1 and Bim protein decreased significantly after adding resveratrol (P<0.05). Conclusion: Resveratrol may improve the inhibitory effect of high glucose on osteoblasts proliferation and differentiation by adjusting SIRT1/FOXO1 pathway. References | Related Articles | Metrics

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Early Implant Restoration Guided by Digital Surgical Guide Plate after Anterior Alveolar Fracture HAN Chunyu, WANG Chuyang, CAI Qing, LI Baosheng, ZHANG Huiyan, LI Zuntai, QI Xiaoshuang, MENG Weiyan 2020, 36(1): 85-86.  DOI: 10.13701/j.cnki.kqyxyj.2020.01.020 Abstract ( 324 )   PDF (1850KB) ( 303 )   For implant prostheses in the aesthetic area, there are stricter requirements about accuracy and long-term stability of implants. In this case, digital guide plate combined with GBR treatment obtained satisfactory prosthesis effect in the anterior area References | Related Articles | Metrics

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Acute Apical Periodontitis of Primary Molar with Odontogenic Maxillary Sinusitis and Infection of Infraorbital Space: A Case Report ZHANG Lijuan, JIANG Ming, LI Shanshan, LI Fan, ZHANG Ying, TAN Kaixuan, LU Jie, YANG Fang 2020, 36(1): 87-88.  DOI: 10.13701/j.cnki.kqyxyj.2020.01.021 Abstract ( 339 )   PDF (2678KB) ( 350 )   Odontogenic maxillary sinusitis and infection of infraorbital space which serve as complications of acute apical periodontitis of primary molar in children are rare. If untreated, they may cause orbital cellulitis, cavernous sinus thrombosis, or even death. References | Related Articles | Metrics